Title:
PHOTOREGULATED PEPTIDE, AND METHOD FOR REGULATION OF PEPTIDE-PROTEIN COMPLEX FORMATION USING THE PHOTOREGULATED PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2007/021016
Kind Code:
A1
Abstract:
A method for photoregulating the formation of a complex between a protein of interest
and a peptide capable of recognizing the protein, wherein a structural change
in the peptide that is used for the recognition of the protein is photoregulated;
a peptide having a cross-linkage in its molecule via a photocleavable cross-linking
group, and a method for production of the peptide; a peptide which has a cross-linkage
in its molecule via a photocleavable cross-linking group and having a cyclic
structure formed together with the cross-linking group and comprises a peptide
having an epitope corresponding to a functional protein or ligand at a region
that forms the cyclic structure, and a method for production of the peptide; and
a reaction regulation method comprising the steps of: irradiating the peptide
comprising the peptide having the epitope with light to cause the dissociation
of the photocleavable cross-liking group, thereby dissociating the cyclic
structure; and initiating the reaction between the peptide having the dissociated
cyclic structure with the functional protein or ligand to form a complex.
Inventors:
HIROTA SHUN (JP)
HAMAZAKI YUJI (JP)
HAMAZAKI YUJI (JP)
Application Number:
PCT/JP2006/316278
Publication Date:
February 22, 2007
Filing Date:
August 14, 2006
Export Citation:
Assignee:
SHIMADZU CORP (JP)
HIROTA SHUN (JP)
HAMAZAKI YUJI (JP)
HIROTA SHUN (JP)
HAMAZAKI YUJI (JP)
International Classes:
C07K1/10; C07K2/00; C07K7/04; C07K7/54; C07K14/00; C07K17/14; G01N33/483; G01N33/566; G01N33/58
Foreign References:
| US5100985A | 1992-03-31 |
Other References:
MARRIOTT G. ET AL.: "Synthesis and applications of heterobifunctional photo cleavage cross-linking reagents", METHODS IN ENZYMOLOGY, vol. 291, 1998, pages 155 - 175, XP008045118
HIROTA S.: "Hikari Kairisei Shushokuki o Mochiita Tanpakushitsu no Folding Han'no no Tsuiseki", BIOPHYSICS, vol. 45, no. 4, 25 July 2005 (2005-07-25), pages 207 - 210, XP003008769
HIROTA S.: "Hikari Kairisei O-Nitrobenzyl-ki Donyu ni yoru Cytochrome c no Oretatami Han'no no Kaimei", RIKEN SYMPOSIA - YUKI KAGAKU HAN'NO NO SHINTENKAI (DAI 2 KAI) - KOEN YOSHISHU (ESSENTIAL HAN'NO KENKYU HOKOKUSHO), 4 December 2000 (2000-12-04), pages 49 - 53, XP003008770
HIROTA S. ET AL.: "Hikari Kairisei Shushokuki Donyu ni yoru Plast Cyanine no Oritatami no Seigyo", DAI 1 KAI SEITAI KINO KANREN BIO TECHNOLOGY BUKAI GODO SYMPOSIUM KOEN YOSHISHU, 1 October 2003 (2003-10-01), pages 74 - 75, XP003008771
HIROTA S. ET AL.: "Hikari Kairisei o-nitrobenzyl Yudotai no Donyu ni yoru Cytochrome c no Kozo Henka no Kenkyu", BIOPHYSICS, vol. 39, no. 1, 2 September 1999 (1999-09-02), pages S160, XP003008772
AKIYAMA M. ET AL.: "Hikari Kairisei 4,5-dimethoxy-2-nitrobenzyl Yudotai no Donyu ni yoru Plast Cyanine no Kozo Henka no Kenkyu", CSJ: THE CHEMICAL SOCIETY OF JAPAN 81ST SPRING MEETING, no. 2F4-04, 11 March 2002 (2002-03-11), pages 903, XP003008773
HIROTA S.: "Hikari Kairisei Shushokuki o Mochiita Tanpakushitsu no Folding Han'no no Tsuiseki", BIOPHYSICS, vol. 45, no. 4, 25 July 2005 (2005-07-25), pages 207 - 210, XP003008769
HIROTA S.: "Hikari Kairisei O-Nitrobenzyl-ki Donyu ni yoru Cytochrome c no Oretatami Han'no no Kaimei", RIKEN SYMPOSIA - YUKI KAGAKU HAN'NO NO SHINTENKAI (DAI 2 KAI) - KOEN YOSHISHU (ESSENTIAL HAN'NO KENKYU HOKOKUSHO), 4 December 2000 (2000-12-04), pages 49 - 53, XP003008770
HIROTA S. ET AL.: "Hikari Kairisei Shushokuki Donyu ni yoru Plast Cyanine no Oritatami no Seigyo", DAI 1 KAI SEITAI KINO KANREN BIO TECHNOLOGY BUKAI GODO SYMPOSIUM KOEN YOSHISHU, 1 October 2003 (2003-10-01), pages 74 - 75, XP003008771
HIROTA S. ET AL.: "Hikari Kairisei o-nitrobenzyl Yudotai no Donyu ni yoru Cytochrome c no Kozo Henka no Kenkyu", BIOPHYSICS, vol. 39, no. 1, 2 September 1999 (1999-09-02), pages S160, XP003008772
AKIYAMA M. ET AL.: "Hikari Kairisei 4,5-dimethoxy-2-nitrobenzyl Yudotai no Donyu ni yoru Plast Cyanine no Kozo Henka no Kenkyu", CSJ: THE CHEMICAL SOCIETY OF JAPAN 81ST SPRING MEETING, no. 2F4-04, 11 March 2002 (2002-03-11), pages 903, XP003008773
Attorney, Agent or Firm:
OKADA, Masahiro (12th Shinko Bldg. 10F 1-6, Zaimoku-cho, Chuo-k, Osaka-shi Osaka, JP)
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