Title:
PHOTORESPONSIVE CELL FIXING AGENT
Document Type and Number:
WIPO Patent Application WO/2019/230441
Kind Code:
A1
Abstract:
[Problem] The present invention addresses the problem of developing a photoresponsive cell fixing agent that can be used repeatedly and continuously, and can selectively and reversibly control the fixing and releasing of cells by photostimulation, regardless of the targeted cell type. [Solution] A photoresponsive cell fixing agent for fixing prescribed target cells on a substrate, said photoresponsive cell fixing agent being characterized by having a hydrophobic chain that can interact with the target cells, a hydrophilic chain that can be arranged in a monomolecular film shape on a surface of the substrate, and a branched linker chain that links the hydrophobic chain and the hydrophilic chain, wherein the photoresponsive cell fixing agent has, on a side chain of the branched linker chain, a photoisomerization site that is isomerized by light irradiation and can change from hydrophobic to hydrophilic.
Inventors:
YAMAGUCHI SATOSHI (JP)
IZUTA SHIN (JP)
OKAMOTO AKIMITSU (JP)
IZUTA SHIN (JP)
OKAMOTO AKIMITSU (JP)
Application Number:
PCT/JP2019/019632
Publication Date:
December 05, 2019
Filing Date:
May 17, 2019
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C12N5/00; C07K5/02; C07K7/02
Domestic Patent References:
| WO2016158327A1 | 2016-10-06 |
Other References:
HE, DI ET AL.: "A photoresponsive soft interface reversibly controls wettability and cell adhesion by conformational changes in a spiropyran- conjugated amphiphilic block copolymer", ACTA BIOMATERIALIA, vol. 51, 2017, pages 101 - 111, XP029943185, DOI: 10.1016/j.actbio.2017.01.049
TADA, YUICHI ET AL.: "Development of a photoresponsive cell culture surface: regional enhancement of living- cell adhesion induced by local light irradiation", JOURNAL OF APPLIED POLYMER SCIENCE, vol. 100, no. 1, 26 January 2006 (2006-01-26) - 5 April 2006 (2006-04-05), pages 495 - 499, XP055662472
BRIEKE, C. ET AL.: "Light-controlled tools", ANGEW. CHEM. INT. ED., vol. 51, no. 34, 2012, pages 8446 - 8476, XP055160738, DOI: 10.1002/anie.201202134
TADA, YUICHI ET AL.: "Development of a photoresponsive cell culture surface: regional enhancement of living- cell adhesion induced by local light irradiation", JOURNAL OF APPLIED POLYMER SCIENCE, vol. 100, no. 1, 26 January 2006 (2006-01-26) - 5 April 2006 (2006-04-05), pages 495 - 499, XP055662472
BRIEKE, C. ET AL.: "Light-controlled tools", ANGEW. CHEM. INT. ED., vol. 51, no. 34, 2012, pages 8446 - 8476, XP055160738, DOI: 10.1002/anie.201202134
Attorney, Agent or Firm:
ONO Makoto et al. (JP)
Download PDF: