Title:
PHOTORESPONSIVE NUCLEIC ACID APTAMER, PHOTOACTIVABLE DNA POLYMERASE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/031801
Kind Code:
A1
Abstract:
Provided are a photoresponsive nucleic acid aptamer, a photoactivable DNA polymerase, a kit, a method for preparing the photoactivable DNA polymerase, a method for activating the photoactivable DNA polymerase, and use of the photoactivable DNA polymerase. The photoresponsive nucleic acid aptamer comprises an aptamer nucleotide sequence and a photocleavable group introduced into the aptamer nucleotide sequence. The photocleavable group is configured for being cleaved upon ultraviolet irradiation to cleave the aptamer nucleotide sequence into two nucleotide sequences. The aptamer nucleotide sequence comprises a G-quadruplex nucleotide sequence capable of forming a G-quadruplex and a side nucleotide sequence capable of forming a hairpin structure. The G-quadruplex nucleotide sequence is configured for binding to a G-quadruplex-binding peptide fused with a DNA polymerase. The side nucleotide sequence is configured for binding to an active site of the DNA polymerase. The use of the photoactivable DNA polymerase provided herein can reduce the difference in amplification time between samples and avoid non-specific amplification, thereby improving the accuracy of nucleic acid detection.
Inventors:
ZHENG KEWEI (CN)
CAI TINGTING (CN)
WANG CHENGLIN (CN)
CHEN YAOQING (CN)
WANG JIAQI (CN)
ZHONG CAILING (CN)
CAI TINGTING (CN)
WANG CHENGLIN (CN)
CHEN YAOQING (CN)
WANG JIAQI (CN)
ZHONG CAILING (CN)
Application Number:
PCT/CN2022/121959
Publication Date:
February 15, 2024
Filing Date:
September 28, 2022
Export Citation:
Assignee:
UNIV SUN YAT SEN (CN)
SHENZHEN QIYU BIOTECHNOLOGY CO LTD (CN)
SHENZHEN QIYU BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/115; C12N9/12; C12Q1/6806; C12Q1/6848
Domestic Patent References:
| WO2021231687A1 | 2021-11-18 |
Foreign References:
| CN114540324A | 2022-05-27 | |||
| US20200123547A1 | 2020-04-23 | |||
| US20200181618A1 | 2020-06-11 |
Other References:
ANDREA MILELLI: "Naphthalene diimide-polyamine hybrids as antiprolifera- tive agents: Focus on the architecture of the polyamine chains", EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, vol. 128, 10 March 2017 (2017-03-10), ISSN: 0223-5324
15 July 2012 (2012-07-15), ISSN: 1674-022X
15 July 2012 (2012-07-15), ISSN: 1674-022X
Attorney, Agent or Firm:
SHENZHEN DISI-ELITE INTELLECTUAL PROPERTY AGENCY (CN)
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