Title:
PHOTORESPONSIVE NUCLEOSIDES AND PHOSPHORAMIDITES
Document Type and Number:
WIPO Patent Application WO/2000/014102
Kind Code:
A1
Abstract:
Photoresponsive substances for introducing photoresponsive nucleosides into strands of DNA without structural freedom and easily. Specifically, nucleic acids bearing photoresponsive groups linked thereto by triple bonds or reactive derivatives of the same, preferably nucleic acids substituted with groups represented by general formula (I), (wherein R is hydrogen or cyano) or reactive derivatives of the same; nucleic acid oligomers containing the above nucleic acids; phosphoramidite reagents comprising phosphoramidite derivatives of the above nucleic acids; and a process for the preparation of nucleic acid oligomers by using the phosphoramidite reagents.
Inventors:
Saito, Isao (1-21, Kanshuji Shibayama Yamashina-ku Kyoto-shi Kyoto-fu, 607-8242, JP)
Dohno, Chikara (779-7, Shin-machi Yamatokohriyama-shi Nara-ken, 639-1054, JP)
Fujisawa, Kazuhiko (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
Nakatani, Kazuhiko (8-10, Todoh Tanisagari Uji-shi Kyoto-fu, 611-0013, JP)
Nakamura, Takashi (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
Dohno, Chikara (779-7, Shin-machi Yamatokohriyama-shi Nara-ken, 639-1054, JP)
Fujisawa, Kazuhiko (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
Nakatani, Kazuhiko (8-10, Todoh Tanisagari Uji-shi Kyoto-fu, 611-0013, JP)
Nakamura, Takashi (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
Application Number:
PCT/JP1999/004898
Publication Date:
March 16, 2000
Filing Date:
September 09, 1999
Export Citation:
Assignee:
JAPAN SCIENCE AND TECHNOLOGY CORPORATION (1-8 Honcho 4-chome Kawaguchi-shi Saitama-ken, 332-0012, JP)
Saito, Isao (1-21, Kanshuji Shibayama Yamashina-ku Kyoto-shi Kyoto-fu, 607-8242, JP)
Dohno, Chikara (779-7, Shin-machi Yamatokohriyama-shi Nara-ken, 639-1054, JP)
Fujisawa, Kazuhiko (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
Nakatani, Kazuhiko (8-10, Todoh Tanisagari Uji-shi Kyoto-fu, 611-0013, JP)
Nakamura, Takashi (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
Saito, Isao (1-21, Kanshuji Shibayama Yamashina-ku Kyoto-shi Kyoto-fu, 607-8242, JP)
Dohno, Chikara (779-7, Shin-machi Yamatokohriyama-shi Nara-ken, 639-1054, JP)
Fujisawa, Kazuhiko (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
Nakatani, Kazuhiko (8-10, Todoh Tanisagari Uji-shi Kyoto-fu, 611-0013, JP)
Nakamura, Takashi (Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering Kyoto University Yoshida Hon-machi Sakyo-ku Kyoto-shi, Kyoto-fu, 606-8501, JP)
International Classes:
C12N15/09; C07H19/04; C07H19/06; C07H21/00; C07H21/04; (IPC1-7): C07H21/00; C07H19/06
Attorney, Agent or Firm:
Saeki, Norio (9th floor, Taka-ai Building 15-2, Nihonbashi 3-chome Chuo-ku Tokyo, 103-0027, JP)