Title:
PHYTASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2020/168943
Kind Code:
A1
Abstract:
The present invention relates to the technical field of biology, in particular to a phytase mutant, a preparation method therefor and an application thereof, a DNA molecule encoding the phytase mutant, a vector, and a host cell. The mutant provided by the present invention contains the substituent of an amino acid at at least one position selected from the following group: 36, 69, 89, 91, 111, 202, 213, 225, 238, 243, 253, 258, and 266. The heat resistance of the mutant is significantly improved, thereby facilitating the wide application of the phytase in feed.
Inventors:
WU XIUXIU (CN)
HUANG YIJUN (CN)
LIU YANG (CN)
ZHANG XIA (CN)
CHENG SIDA (CN)
HUANG YIJUN (CN)
LIU YANG (CN)
ZHANG XIA (CN)
CHENG SIDA (CN)
Application Number:
PCT/CN2020/074735
Publication Date:
August 27, 2020
Filing Date:
February 11, 2020
Export Citation:
Assignee:
QINGDAO VLAND BIOTECH GROUP CO LTD (CN)
International Classes:
C12N9/16; C12N15/55; C12N15/63
Foreign References:
| CN102002487A | 2011-04-06 |
Other References:
WANG, XI ET AL.: "Improving Thermostability of Escherichia coli phytase AppA by Multipoint Mutation", HIGH TECHNOLOGY LETTERS, vol. 24, no. 12, 31 December 2014 (2014-12-31), pages 1306 - 1313, XP055849164
YAO MZ: "Improving the thermostability of Escherichia coli phytase, appA, by enhancement of glycosylation", BIOTECHNOL LETT, 31 October 2013 (2013-10-31), XP055474877
"Genbank", Database accession no. ABF60232
SAMBROOK: "MOLECULAR CLONING: A LABORATORY MANUAL", 2001
AUSUBEL: "CURRENT PROTOCOLS IN MOLECULAR BIOLOGY", 2003
YAO MZ: "Improving the thermostability of Escherichia coli phytase, appA, by enhancement of glycosylation", BIOTECHNOL LETT, 31 October 2013 (2013-10-31), XP055474877
"Genbank", Database accession no. ABF60232
SAMBROOK: "MOLECULAR CLONING: A LABORATORY MANUAL", 2001
AUSUBEL: "CURRENT PROTOCOLS IN MOLECULAR BIOLOGY", 2003
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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