Title:
PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
Document Type and Number:
WIPO Patent Application WO2002095003
Kind Code:
A3
Abstract:
The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E.coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
Inventors:
SHORT JAY M (US)
KRETZ KEITH (US)
GRAY KEVIN A (US)
BARTON NELSON R (US)
GARRETT JAMES B (US)
O'DONOGHUE EILEEN (US)
MATHUR ERIC J (US)
KRETZ KEITH (US)
GRAY KEVIN A (US)
BARTON NELSON R (US)
GARRETT JAMES B (US)
O'DONOGHUE EILEEN (US)
MATHUR ERIC J (US)
Application Number:
PCT/US2002/016482
Publication Date:
September 23, 2004
Filing Date:
May 24, 2002
Export Citation:
Assignee:
DIVERSA CORP (US)
SHORT JAY M (US)
KRETZ KEITH (US)
GRAY KEVIN A (US)
BARTON NELSON R (US)
GARRETT JAMES B (US)
O'DONOGHUE EILEEN (US)
MATHUR ERIC J (US)
SHORT JAY M (US)
KRETZ KEITH (US)
GRAY KEVIN A (US)
BARTON NELSON R (US)
GARRETT JAMES B (US)
O'DONOGHUE EILEEN (US)
MATHUR ERIC J (US)
International Classes:
A01H5/00; A01K67/027; A21D8/04; A23J3/14; A23K1/14; A23K1/16; A23K1/165; A23L1/30; A23L5/20; A23L7/104; A23L11/00; A23L11/30; A23L29/00; C07K16/40; C12M1/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/09; C12P21/08; C12Q1/42; G01N33/53; G01N33/566; A61K38/00; (IPC1-7): C12Q1/34; C12P21/06; C12N9/16; C12N15/00; C12N5/00; C12N1/20; C07H21/04
Domestic Patent References:
| WO2000064247A1 | 2000-11-02 |
Other References:
OSTANIN ET AL: "Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase", J. BIOL. CHEM., vol. 267, no. 32, November 1992 (1992-11-01), pages 22830 - 22836, XP002129674
DATABASE GENBANK [online] 1992, OSTANIN ET AL, XP002979313, Database accession no. (L03371)
DATABASE GENBANK [online] 2 November 2000 (2000-11-02), FORSBERG ET AL, XP002979314, Database accession no. (AAC68299)
DATABASE GENBANK [online] 2 November 2000 (2000-11-02), FORSBERG ET AL, XP002979315, Database accession no. (AAC68296)
DATABASE GENBANK [online] 15 December 1999 (1999-12-15), BAE ET AL, XP002979316, Database accession no. (ABK12514)
DATABASE GENBANK [online] 1992, OSTANIN ET AL, XP002979313, Database accession no. (L03371)
DATABASE GENBANK [online] 2 November 2000 (2000-11-02), FORSBERG ET AL, XP002979314, Database accession no. (AAC68299)
DATABASE GENBANK [online] 2 November 2000 (2000-11-02), FORSBERG ET AL, XP002979315, Database accession no. (AAC68296)
DATABASE GENBANK [online] 15 December 1999 (1999-12-15), BAE ET AL, XP002979316, Database accession no. (ABK12514)
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