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Title:
PLANT CELL HAVING MUTATION INTRODUCED INTO TARGET DNA, AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2015/129686
Kind Code:
A1
Abstract:
 It was discovered that, in a plant cell, a marker gene inserted into the genomic DNA by homologous recombination and having nuclease recognition sites added to both ends thereof can be removed by the corresponding nuclease, and the recognition sites can be removed without leaving a trace by matching the sequence of at least 30 nucleotides adjacent to the nuclease recognition sites. Thus, it is possible, in a method for introducing a mutation into target DNA on a plant cell genome by homologous recombination, to select, in a stable manner and as an indicator of expression of the marker gene, plant cells into which this mutation has been introduced, and it is possible to remove unnecessary sequences such as marker genes and to introduce only the necessary mutation into the target DNA in the selected cells.

Inventors:
IWAKAMI MASAKI (JP)
TOKI SEIICHI (JP)
Application Number:
PCT/JP2015/055230
Publication Date:
September 03, 2015
Filing Date:
February 24, 2015
Export Citation:
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Assignee:
NAT INST OF AGROBIO SCIENCES (JP)
International Classes:
C12N15/09; A01H1/00; A01H5/00; C12N5/10
Other References:
AYAKO YOKOI ET AL.: "The development of targeted genome engineering in plants", REGULATION OF PLANT GROWTH & DEVELOPMENT, vol. 48, no. 2, 20 December 2013 (2013-12-20), pages 117 - 124
MASAKI ENDO ET AL.: "Shokubutsu Genome no Hyoteki Idenshi Kaihen ni Okeru Jinko Seigen Koso no Riyo", BIOTECHNOLOGY, vol. 91, no. 8, 25 August 2013 (2013-08-25), pages 348 - 351
AYAKO YOKOI ET AL.: "Ine no Hyoteki Idenshi Kaihen Gijutsu no Tenkai", DAI 31 KAI JAPANESE SOCIETY FOR PLANT CELL AND MOLECULAR BIOLOGY (SAPPORO) TAIKAI SYMPOSIUM KOEN YOSHISHU, 20 August 2013 (2013-08-20), pages 56
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
Patent business corporation cent Crest international patent firm (JP)
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