Title:
PLANTS TRANSFORMED WITH THIOREDOXIN
Document Type and Number:
WIPO Patent Application WO/2000/058453
Kind Code:
A2
Abstract:
Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.
Inventors:
CHO MYEONG-JE (US)
LEMAUX PEGGY G (US)
BUCHANAN BOB B (US)
WONG JOSHUA (US)
MARX CORINA (US)
LEMAUX PEGGY G (US)
BUCHANAN BOB B (US)
WONG JOSHUA (US)
MARX CORINA (US)
Application Number:
PCT/US2000/008315
Publication Date:
October 05, 2000
Filing Date:
March 29, 2000
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
CHO MYEONG JE (US)
LEMAUX PEGGY G (US)
BUCHANAN BOB B (US)
WONG JOSHUA (US)
MARX CORINA (US)
CHO MYEONG JE (US)
LEMAUX PEGGY G (US)
BUCHANAN BOB B (US)
WONG JOSHUA (US)
MARX CORINA (US)
International Classes:
A01H1/00; A01H5/00; A01H4/00; A01H5/10; A21D13/00; A21D13/06; A21D13/08; A23G3/00; A23G3/34; A23J1/12; A23K1/00; A23K1/14; A23K1/16; A23L1/00; A23L1/30; A23L7/109; A23L29/00; A61K36/899; A61P37/08; C07K14/415; C12C1/18; C12N5/10; C12N9/02; C12N15/09; C12N15/53; C12N15/82; C12P7/06; (IPC1-7): C12N15/00
Domestic Patent References:
| WO1996003505A1 | 1996-02-08 |
Foreign References:
| US5792506A | 1998-08-11 |
Other References:
CHO ET AL: "Subcellular targeting of barley hordein promoter-uidA fusions in transgenic barley seed" IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL,US,TISSUE CULTURE ASSOCIATION, COLUMBIA, MD, vol. 34, no. 3, PART 02, 1998, page 48A XP002096845 ISSN: 1071-2690
BUCHANAN B B ET AL: "Thioredoxin: A multifunctional regulatory protein with a bright future in technology and medicine" ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,US,NEW YORK, US, vol. 314, no. 2, 1 November 1994 (1994-11-01), pages 257-260, XP002089742 ISSN: 0003-9861
BESSE ET AL.: "Thiocalsin: A thioredoxin-linked, substrate-specific protease dependent on calcium" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, vol. 93, April 1996 (1996-04), pages 3169-3175, XP002154263 cited in the application
BUCHANAN B B ET AL: "THIOREDOXIN AS A REDUCTANT OF STORAGE PROTEINS AND AN IMPROVER OF PRODUCTS FROM DIFFERENT CEREAL FLOURS" CEREAL SCIENCE TODAY,US,AMERICAN ASSOCIATION OF CEREAL CHEMISTS,, vol. 38, no. 8, 1993, page 612 XP000886094 ISSN: 0009-0360
DEL VAL G ET AL: "Disulfide bond reduction by thioredoxin alleviates the allergenicity of ragweed pollen" JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 26 February 1999 (1999-02-26), XP000907177
BUCHANAN B B ET AL: "Thioredoxin: A multifunctional regulatory protein with a bright future in technology and medicine" ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,US,NEW YORK, US, vol. 314, no. 2, 1 November 1994 (1994-11-01), pages 257-260, XP002089742 ISSN: 0003-9861
BESSE ET AL.: "Thiocalsin: A thioredoxin-linked, substrate-specific protease dependent on calcium" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, vol. 93, April 1996 (1996-04), pages 3169-3175, XP002154263 cited in the application
BUCHANAN B B ET AL: "THIOREDOXIN AS A REDUCTANT OF STORAGE PROTEINS AND AN IMPROVER OF PRODUCTS FROM DIFFERENT CEREAL FLOURS" CEREAL SCIENCE TODAY,US,AMERICAN ASSOCIATION OF CEREAL CHEMISTS,, vol. 38, no. 8, 1993, page 612 XP000886094 ISSN: 0009-0360
DEL VAL G ET AL: "Disulfide bond reduction by thioredoxin alleviates the allergenicity of ragweed pollen" JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 26 February 1999 (1999-02-26), XP000907177
Attorney, Agent or Firm:
Dehlinger, Peter J. (CA, US)
Download PDF:
Claims:
We claim:
| 1. | A transgenic plant, wherein at least a part of said plant has an increased specific activity of thioredoxin in comparison to the same part of a nontransgenic plant of the same species. |
| 2. | The transgenic plant of claim 1, wherein said thioredoxin specific activity is at least twice the specific activity of thioredoxin in said nontransgenic plant of the same species. |
| 3. | The transgenic plant of claim 1, wherein said thioredoxin specific activity is at least five times the specific activity of thioredoxin in said nontransgenic plant of the same species. |
| 4. | The transgenic plant of claim 1, wherein said thioredoxin specific activity is at least ten times the specific activity of thioredoxin in said nontransgenic plant of the same species. |
| 5. | The transgenic plant of claim 1, wherein said thioredoxin is thioredoxin h. |
| 6. | The transgenic plant of claim 6, wherein said thioredoxin h is is barley, wheat, Arabidopsis, tobacco, rice, Brassica, Picea, or soy bean thioredoxin h. |
| 7. | The transgenic plant of claim 1, wherein said specific activity of thioredoxin is at least 0.128 Aonm/min/mg protein. |
| 8. | A transgenic plant, wherein at least a part of said plant has increased thioredoxin protein content in comparison to the thioredoxin protein in the same part of a nontransgenic plant of the same species. |
| 9. | The transgenic plant of claim 8, wherein said thioredoxin protein is thioredoxin h protein. |
| 10. | The transgenic plant of claim 8, wherein said thioredoxin h protein is barley, wheat, Arabidopsis, tobacco, rice, Brassica, Picea, or soy bean thioredoxin h. |
| 11. | The transgenic plant of claim 8, wherein said thioredoxin protein content is at least 10, ug/mg soluble protein. |
| 12. | A transgenic plant, wherein at least part of said plant has an increased SH: SS ratio in comparison to the same part of a nontransgenic plant of the same species. |
| 13. | The transgenic plant of claim 12 wherein said SH: SS ratio is at least 5: 1. |
| 14. | The transgenic plant of claims 1,8, or 12 wherein said plant is selected from the group consisting of rice, barley, maize, wheat, oat, rye, sorghum, millet, triticale, and forage and turf grass. |
| 15. | A transgenic plant, wherein at least part of said plant has reduced allergenicity in comparison to the same part of a nontransgenic plant of the same species. |
| 16. | The transgenic plant of claim 15 wherein said allergenicity is hypersensitivity, wherein said hypersensitivity is reduced by at least 5%. |
| 17. | A transgenic plant, wherein at least part of said plant has an increased digestibility in comparison to the same part of a nontransgenic plant of the same species. |
| 18. | The transgenic plant of claim 17 wherein said digestibility is increased by at least 5%. |
| 19. | The transgenic plant of claim 15 or 17, wherein said transgenic plant is selected from the group consisting of rice, barley, maize, wheat, oat, rye, sorghum, millet, triticale, and forage and turf grass. |
| 20. | A transgenic plant, wherein at least part of said plant has an earlier onset and/or an increased expression of a gibberellic acid inducible enzyme in comparison to the same part of a non transgenic plant of the same species. |
| 21. | The transgenic plant of claim 20 wherein said enzyme is pullulanase. |
| 22. | The transgenic plant of claim 21 wherein said pullulanase has a specific activity of at least 12 absorbance units at 534 nm/30 min/mg protein. |
| 23. | The transgenic plant of claim 20 wherein said enzyme is alphaamylase. |
| 24. | The transgenic plant of claim 23, wherein said alphaamylase is expressed in said plant is at least 8 hours before expression in said nontransgenic plant of the same species. |
| 25. | The transgenic plant of claim 23 wherein said alphaamylase is alphaamylase A. |
| 26. | The transgenic plant of claim 23 wherein said alphaamylase is alphaamylase B. |
| 27. | The transgenic plant of claim 20, wherein said transgenic plant is barley. |
| 28. | The transgenic plant of claims 17, or 20 wherein said part is an edible part. |
| 29. | The transgenic plant of claim 28, wherein said edible part is a grain. |
| 30. | The transgenic plant of claim 28, wherein said edible part is a seed. |
| 31. | The transgenic plant of claims 1,8,12,15,17, or 20 wherein said part of said transgenic plant comprises a recombinant nucleic acid expressing a thioredoxin polypeptide. |
| 32. | A transgenic plant wherein at least part of said plant comprises a recombinant nucleic acid comprising a promoter active in said part operably linked to a nucleic acid molecule encoding a thioredoxin polypeptide. |
| 33. | The transgenic plant of claim 32 wherein said part is a seed. |
| 34. | The transgenic plant of claim 32 wherein said part is a grain. |
| 35. | The transgenic plant of claim 32 wherein said promoter is a seed or grain maturationspecific promoter. |
| 36. | The transgenic plant of claim 35 wherein said promoter is selected from the group consisting of rice glutelins, rice oryzins, rice prolamines, barley hordeins, wheat gliadins, wheat glutelins, maize zeins, maize glutelins, oat glutelins, sorghum kasirins, millet pennisetins, rye secalins, and a maize embryospecific globulin promoter. |
| 37. | The transgenic plant of claim 36 wherein said barley hordein promoter is selected from the group consisting of B1 hordein and D hordein promoters. |
| 38. | The transgenic plant of claim 32 wherein said plant is selected from the group consisting of rice, barley, maize, wheat, oat, rye, sorghum, millet, triticale, turf grass and forage grass. |
| 39. | The transgenic plant of claim 32 wherein said thioredoxin is thioredoxin h. |
| 40. | The transgenic plant of claim 39 wherein said thioredoxin h is barley, wheat, tobacco, rice, Brassica, Arabidopsis, Picea, or soy bean thioredoxin h. |
| 41. | The transgenic plant of claim 32 wherein said recombinant nucleic acid further comprises a nucleic acid molecule encoding a signal peptide operably linked to said promoter and said nucleic acid molecule encoding a thioredoxin protein. |
| 42. | The transgenic plant of claim 41 wherein said signal peptide targets expression of the thioredoxin polypeptide to an intracellular body. |
| 43. | The transgenic plant of claim 42 wherein said signal peptide is selected from the group consisting of barley B1 hordein and D hordein signal peptides. |
| 44. | A transgenic seed or grain comprising an increased specific activity of thioredoxin in comparison to a nontransgenic seed or grain of the same species. |
| 45. | The transgenic seed or grain of claim 44, wherein said specific activity of thioredoxin is at least twice the specific activity of thioredoxin in said nontransgenic seed or grain of the same species. |
| 46. | The transgenic seed or grain of claim 44, wherein said specific activity of thioredoxin is at least five times the specific activity of thioredoxin in said nontransgenic seed or grain of the same species. |
| 47. | The transgenic seed or grain of claim 44, wherein said specific activity of thioredoxin is at least ten times the activity of thioredoxin in said nontransgenic seed or grain of the same species. |
| 48. | The transgenic seed or grain of claim 44, wherein said thioredoxin is thioredoxin h. |
| 49. | The transgenic seed or grain of claim 48, wherein said thioredoxin h is barley, wheat, tobacco, soy bean, Arabidopsis, Picea, or Brassica thioredoxin h. |
| 50. | The transgenic seed or grain of claim 44, wherein said specific activity of thioredoxin is at least 0.128 A340nm/min/mg protein. |
| 51. | A transgenic seed or grain comprising increased content of thioredoxin polypeptide in comparison to a nontransgenic seed or grain of the same species. |
| 52. | The transgenic seed or grain of claim 51 wherein said content of thioredoxin polypeptide is at least 10 ug/mg soluble protein.. |
| 53. | The transgenic seed or grain of claim 51, wherein said thioredoxin polypeptide is thioredoxin h polypeptide. |
| 54. | The transgenic seed or grain of claim 53, wherein said thioredoxin h polypeptide is barley, wheat, tobacco, rice, Brassica, Picea, soy bean, or Arabidopsis thioredoxin h protein. |
| 55. | A transgenic seed or grain comprising an increased SH: SS ratio in comparison to a non transgenic seed or grain of the same species. |
| 56. | The transgenic seed or grain of claim 55, wherein said SH: SS ratio is at least 5: 1. |
| 57. | The transgenic seed or grain of claims 44,51, or 55 wherein said seed or grain is selected from the group consisting of rice, barley, maize, wheat, oat, rye, sorghum, millet, triticale, forage grass and turf grass. |
| 58. | A transgenic seed or grain comprising reduced allergenicity in comparison to a nontransgenic seed or grain of the same species. |
| 59. | The transgenic seed or grain of claim 58 wherein said allergenicity is hypersensitivity, wherein said hypersensitivity is reduced by at least. 5%. |
| 60. | A transgenic seed or grain comprising increased digestibility in comparison to a nontransgenic seed or grain of the same species. |
| 61. | The transgenic seed or grain of claim 60 wherein said digestibility is increased by at least 5%. |
| 62. | The transgenic seed or grain of claim 58 or 60, wherein said transgenic seed or grain is wheat, barley, rice, maize, oat, rye sorghum, millet, triticale, forage grass or turf grass seed or grain. |
| 63. | A transgenic seed or grain overexpressing a thioredoxin protein comprising an earlier onset and/or an increased expression of a gibberellic inducible enzyme in comparison to a non transgenic seed of the same species. |
| 64. | The transgenic seed or grain of claim 63, wherein said seed or grain is germinated and wherein said enzyme is pullulanase. |
| 65. | The transgenic seed or grain of claim 64 wherein said pullulanase has a specific activity of at least 12 absorbance units at 534 nm/30 min/mg protein. |
| 66. | The transgenic seed or grain of claim 63 wherein said enzyme is alphaamylase. |
| 67. | The transgenic seed or grain of claim 66 wherein said alphaamylase is expressed in said seed or grain at least 8 hours before expression in said nontransgenic plant of the same species. |
| 68. | The transgenic seed or grain of claim 66 wherein said alphaamylase is alphaamylase A. |
| 69. | The transgenic seed or grain of claim 66 wherein said alphaamylase is alphaamylase B. |
| 70. | A transgenic seed or grain having an increased germination rate in comparison to a non transgenic seed or grain of the same species. |
| 71. | A transgenic seed or grain having an onset of germination that occurs at least 8 hours prior to the onset of germination of a nontransgenic seed or grain of the same species. |
| 72. | A transgenic seed or grain, wherein said seed or grain germinates 5% more efficiently than a nontransgenic seed or grain of the same species. |
| 73. | The transgenic seed or grain of claim 63,70,71, or 72 wherein said transgenic seed or grain is a barley seed. |
| 74. | A transgenic seed or grain overexpressing a thioredoxin, wherein protein of said seed or grain are redistributed at least 5% greater to the soluble fraction in comparison to a nontransgenic seed or grain of the same species. |
| 75. | The transgenic seed or grain of claim 74, wherein said transgenic seed or grain is a wheat, barley, rice, maize, oat, rye, sorghum, millet, triticale, forage grass or turf grass seed or grain. |
| 76. | The transgenic seed or grain of claim 44,51,55,58,60,63,70,71,27, or 74 wherein said transgenic seed or grain comprises a recombinant nucleic acid expressing a thioredoxin polypeptide. |
| 77. | A transgenic seed or grain comprising a recombinant nucleic acid comprising a promoter active in said seed or grain operably linked to a nucleic acid molecule encoding a thioredoxin polypeptide. |
| 78. | The transgenic seed or grain of claim 77 wherein said promoter is a seed or grain maturation specific promoter. |
| 79. | The transgenic seed or grain of claim 78 wherein said promoter is selected from the group consisting of rice glutelins, rice oryzins, rice prolamines, barley hordeins, wheat gliadins, wheat glutelins, maize zeins, maize glutelins, oat glutelins, sorghum kasirins, millet pennisetins, rye secalins, and a maize embryospecific globulin. |
| 80. | The transgenic seed or grain of claim 79 wherein said barley hordein promoter is selected from the group consisting of B1 hordein and D hordein promoters. |
| 81. | The transgenic seed or grain of claim 80 wherein said seed or grain is selected from the group consisting of rice, barley, maize, wheat, oat, rye, sorghum, millet, and triticale seed or grain. |
| 82. | The transgenic seed or grain of claim 77 wherein said thioredoxin polypeptide is thioredoxin h. |
| 83. | The transgenic seed or grain of claim 82 wherein said thioredoxin h is barley, wheat, tobacco, rice, soy bean, Brassica, Picea, or Arabidopsis thioredoxin h. |
| 84. | The transgenic seed or grain of claim 77 wherein said recombinant nucleic acid further comprises a nucleic acid molecule encoding a signal peptide operably linked to said promoter and said nucleic acid molecule encoding a thioredoxin protein. |
| 85. | The transgenic seed or grain of claim 84 wherein said signal peptide targets expression of the thioredoxin polypeptide to an intracellular body. |
| 86. | The transgenic seed or grain of claim 85 wherein said signal peptide is selected from the group consisting of barley B1 hordein and D hordein signal peptides. |
| 87. | A food, feed or beverage product made from the transgenic seed or grain according to claim 44, 71,77, or 84. |
| 88. | The food, feed, or beverage product of claim 87 wherein said product is flour, dough, bread, pasta, cookies, cake, thickener, beer, malted beverage, or a food additive. |
| 89. | The food, feed, or beer product of claim 87 wherein said product has reduced allergenicity. |
| 90. | The food, feed, or beverage product of claim 89 wherein said product has increased digestibility. |
| 91. | The food product of claim 88 wherein said dough has increased strength and volume in comparison to a dough made from a nontransgenic seed or grain of the same species. |
| 92. | The food product of claim 91 wherein said dough has an increased volume of at least 3% in comparison to a dough made from a nontransgenic seed or grain of the same species. |
| 93. | A transgenic plant produced from the seed or grain according to claim 44,51,55,58,60,63,70, 71,77, or 84. |
| 94. | A method of making a transgenic plant comprising cultivating the transgenic seed or grain according to claim 44,51,55,58,60,63,70,71,77, or 84. |
| 95. | A method of making a transgenic plant, wherein at least a part of said plant has an increased thioredoxin specific activity in comparison to a nontransgenic plant of the same species, comprising: expressing in said part a recombinant nucleic acid comprising a nucleic acid molecule encoding a thioredoxin polypeptide operably linked to transcription regulatory elements active in said part. |
| 96. | A method of making a transgenic plant, wherein at least a part of said plant has increased thioredoxin protein content in comparison to a nontransgenic plant of the same species, comprising: expressing in said part a recombinant nucleic acid comprising a nucleic acid molecule encoding a thioredoxin polypeptide operably linked to transcription regulatory elements active in said part. |
| 97. | A method of making a transgenic plant, wherein at least a part of said plant has an increased SH: SS ratio in comparison to a nontransgenic plant of the same species, comprising: expressing in said part a recombinant nucleic acid comprising a nucleic acid molecule encoding a thioredoxin polypeptide operably linked to transcription regulatory elements active in said part. |
| 98. | A method of making a transgenic plant, wherein at least a part of said plant is less allergenic in comparison to a nontransgenic plant of the same species, comprising: expressing in said part a recombinant nucleic acid comprising a nucleic acid molecule encoding a thioredoxin polypeptide operably linked to transcription regulatory elements active in said part. |
| 99. | A method of making a transgenic plant, wherein at least a part of said plant has an increased digestibility in comparison to a nontransgenic plant of the same species, comprising: expressing in said part a recombinant nucleic acid comprising a nucleic acid molecule encoding a thioredoxin polypeptide operably linked to transcription regulatory elements active in said part. |
| 100. | A method of making a transgenic plant, wherein at least a part of said plant has an altered expression of a gibberellic acid inducible enzyme in comparison to a nontransgenic plant of the same species, comprising: expressing in said part a recombinant nucleic acid comprising a nucleic acid molecule encoding a thioredoxin polypeptide operably linked to transcription regulatory elements active in said part. |
| 101. | The method according to any one of claims 95100, wherein said part is a seed or grain. |
| 102. | A method of altering the germination characteristics of a seed or grain comprising: germinating a seed comprising a recombinant nucleic acid comprising a nucleic acid molecule encoding a thioredoxin polypeptide operably linked to transcription regulatory elements active in said seed or grain. |
| 103. | A method of alleviating or preventing a malabsorption syndrome or an allergy comprising feeding a patient having said syndrome or allergy a food product made from the transgenic seed or grain according to claim or 84. |
| 104. | A method of producing alcohol comprising: fermenting the transgenic seed or grain according to claim 44,51,55,58,60,63,70,71,77, or 84. |
| 105. | A method of producing malt comprising: germinating the transgenic seed or grain according to claim 44,51,55,58,60,63,70,71,77, or 84. under conditions whereby malt is produced. |
| 106. | A method of purifying thioredoxin comprising: heating an extract of a transgenic plant, wherein said plant has increased thioredoxin protein in comparison to a nontransgenic plant of the same species, whereby NADPH oxidation of said extract is substantially decreased and said thioredoxin is purified. |
| 107. | The method of claim 106 wherein said thioredoxin protein is active thioredoxin protein. |
| 108. | A food, feed, or beverage comprising a part of a transgenic plant or an extract of said part wherein said part comprises a recombinant nucleic acid comprising a sequence encoding a thioredoxin operably linked to transcription regulatory sequence operable in said part. |
| 109. | The food, feed, or beverage of claim 108 wherein said food, feed, or beverage has hyperdigestible protein. |
| 110. | The food, feed, or beverage of claim 108 wherein said food, feed, or beverage has hyperdigestible starch. |
| 111. | The food, feed, or beverage of claim 108 wherein said food, feed, or beverage is hypoallergenic. |
Description:
INTERNATIONALSEARCHREPORT i-------------------) Interrna)ApplicationNo PCT/US00/08315 C.(Continuation)DOCUMENTSCONSIDEREDTOBERELEVANT Category ° Citationofdocument,withindication,whereappropriate,oftherele
vantpassagesRelevanttoclaimNo. 57-60, 62-64, 66-70, 73, 76-91, 93-105, 108-111 page8,line7-page9,line9 page9,line21-line30 page10,line10-line18 claims6-8,18 YCHOETAL:"Subcellular targetingof1,5,6, barleyhordeinpromoter-uidAfusionsin8-10,12, transgenicbarleyseed"14,15, INVITROCELLULAR&DEVELOPMENTALBIOLOGY.17, ANIMAL,US,TISSUECULTUREASSOCIATION,19-21, COLUMBIA,MD,23, vol.34,no.3,PART02,1998,page48A25-44, XP00209684548,49, ISSN:1071-269051, 53-55, 57-60, 62-64, 66-70, 73, 76-91, 93-105, 108-111 thewholedocument ABUCHANANBBETAL:"Thioredoxin:A66-69, multifunctionalregulatoryproteinwitha91, brightfuture intechnologyandmedicine"108-111 ARCHIVESOFBIOCHEMISTRYAND BIOPHYSICS,US,NEWYORK,US, vol.314,no.2, 1November1994(1994-11-01),pages 257-260,XP002089742 ISSN:0003-9861 page259-page260 ABESSE ET AL. :"Thiocalsin:A63-69, thioredoxin-linked,substrate-specific87-105, proteasedependentoncalcium"108-111 PROCEEDINGSOFTHENATIONALACADEMYOF SCIENCE, vol.93,April1996(1996-04),pages 3169-3175,XP002154263 citedintheapplication thewholedocument AUS5792506A(LOZANOROSAETAL)87-105, 11August1998(1998-08-11)108-111 thewholedocument 1-/- INTERNATIONALSEARCHREPORT InternialApplicationNo PCT/US00/08315 C.(Continuation)DOCUMENTSCONSIDEREDTOBERELEVANT CategoryCitationofdocument,withindication,whereappropriate,o
ftherelevantpassagesRelevanttoclaimNo. ABUCHANANBBETAL:"THIOREDOXINASA REDUCTANTOFSTORAGEPROTEINSANDAN IMPROVEROFPRODUCTSFROMDIFFERENTCEREAL FLOURS" CEREALSCIENCETODAY,US,AMERICAN ASSOCIATIONOFCEREALCHEMISTS,, vol.38,no.8,1993,page612XP000886094 ISSN:0009-0360 ADELVALGETAL:"Disulfidebond reductionbythioredoxinalleviatesthe allergenicityofragweedpollen" JOURNALOFALLERGYANDCLINICAL IMMUNOLOGY, 26February1999(1999-02-26), XP000907177 1 InternationalApplication No. PCTtS 00 t8315 FURTHERINFORMATIONCONTINUEFROMPCT/ISA/210 ThisInternationalSearchingAuthorityfoundmultiple(groupsof) inventionsinthisinternationalapplication,asfollows: 1.Claims:1-14,28-31(partially),32-57,63-69, 73(partially),74-75,76(partially),77-86, 87-94(partially)95-102,103-105(partially), 106-111 Atransgenicplantand/orseedwithanincreasedspecific activityand/orcontentofthioredoxinandtheiruses. 2.Claims:15-16,28-31(partially),58-59,76(partially), 87-94(partially),103-105(partially) Atransgenicplantand/orseedwithreducedallergenicity andtheiruses. 3.Claims:17-19,28-31(partially),60-62,76(partially), 87-94(partially),103-105(partially) Atransgenicplantand/orseedwithincreaseddigestability andtheiruses. 4.Claims:20-27,28-31(partially) Atransgenicplantand/orseedwithanearlieronsetand/or anincreasedexpressionofgibberellicacidinducibleenzyme. 5.Claims:70-72,73(partially),76(partially), 87-94(partially),103-105(partially) Atransgenicseedand/orgrainwithincreasedgermination rateandtheiruses. InternationalApplication No. PCTSS 00 t8315 FURTHERINFORMATIONCONTINUEFROMPCT/ISA/210 ContinuationofBoxI.1 Althoughclaim103isdirectedtoamethodoftreatmentofthe human/animalbody,thesearchhasbeencarriedoutandbasedonthe allegedeffectsofthecompound/composition. ContinuationofBoxI.1 Rule39.1(iv)PCT-Methodfortreatmentofthehumanoranimalbodyby therapy INTERNATIONALSEARCHREPORT !------------------- Interr nal Application No ....ormation on patent tamilymembersPCT/US 00/08315 PatentdocumentPublication Patent family Publication citedinsearchreportdate member(s)date WO9603505A08-02-1996FR 2723097 A 02-02-1996 AU 3082795 A 22-02-1996 EP 0802977 A 29-10-1997 US5792506A11-08-1998US 6113951 A 05-09-2000 AU 3956395 A 15-05-1996 EP 0784676 A 23-07-1997 JP 10510244 T 06-10-1998 WO 9612799 A 02-05-1996 US 5952034 A 14-09-1999 AT 175312 T 15-01-1999 AU 677771 B 08-05-1997 AU 2861792 A 21-05-1993 CA 2121137 A 29-04-1993 CZ 9400832 A 16-08-1995 DE 69228130 D 18-02-1999 EP 0672127 A 20-09-1995 HU 69780 A 28-09-1995 JP 7502887 T 30-03-1995 NZ 244695 A 26-03-1996 SK 41894 A 11-07-1995 WO 9308274 A 29-04-1993 US 6114504 A 05-09-2000 EP 0863154 A 09-09-1998 ZA 9207831 A 27-04-1993
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