Title:
PLURIPOTENT STEM CELL DERIVED FROM CARDIAC TISSUE
Document Type and Number:
WIPO Patent Application WO/2006/093276
Kind Code:
A1
Abstract:
Disclosed is a stem cell applicable to a regenerative therapy. Also disclosed is a technique for regenerative therapy using the cell. A piece of a cardiac tissue removed is treated with an enzyme to produce a cell suspension. The cells suspension is then subjected to any one of the following procedures (1) to (3) to produce pluripotent cells: (1) isolation of cells by density gradient method; (2) suspension culture in a medium supplemented with a fibroblast growth factor and an epithelial cell growth factor; and (3) selective isolation of cells forming floating spheres. The pluripotent cells can be used to perform a regenerative therapy.
Inventors:
OH HIDEMASA (JP)
TATEISHI KENTO (JP)
MATSUBARA HIROAKI (JP)
TATEISHI KENTO (JP)
MATSUBARA HIROAKI (JP)
Application Number:
PCT/JP2006/304111
Publication Date:
September 08, 2006
Filing Date:
March 03, 2006
Export Citation:
Assignee:
UNIV KYOTO (JP)
OH HIDEMASA (JP)
TATEISHI KENTO (JP)
MATSUBARA HIROAKI (JP)
OH HIDEMASA (JP)
TATEISHI KENTO (JP)
MATSUBARA HIROAKI (JP)
International Classes:
A61K35/34; A61L27/00; A61L27/38; A61L27/58; A61P9/00; A61P41/00; A61P43/00; C12N5/07; C12N5/071; C12N5/074; C12N5/077
Domestic Patent References:
| WO2005003334A2 | 2005-01-13 | |||
| WO2003027281A2 | 2003-04-03 | |||
| WO2004019767A2 | 2004-03-11 |
Other References:
OH H. ET AL.: "Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction", PROC. NATL. ACAD. SCI. USA, vol. 100, no. 21, 2003, pages 12313 - 12318, XP002984517
HIERLIHY A.M. ET AL.: "The post-natal heart contains a myocardial stem cell population", FEBS LETT., vol. 530, no. 1-3, 2002, pages 239 - 243, XP002366595
See also references of EP 1857544A4
OH H.: "Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction", PROC NATL ACAD SCI USA, vol. 100, 14 October 2003 (2003-10-14), pages 12313 - 12318, XP002984517, DOI: doi:10.1073/pnas.2132126100
MESSINA E., ISOLATION AND EXPANSION OF ADULT CARDIAC
HIERLIHY A.M. ET AL.: "The post-natal heart contains a myocardial stem cell population", FEBS LETT., vol. 530, no. 1-3, 2002, pages 239 - 243, XP002366595
See also references of EP 1857544A4
OH H.: "Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction", PROC NATL ACAD SCI USA, vol. 100, 14 October 2003 (2003-10-14), pages 12313 - 12318, XP002984517, DOI: doi:10.1073/pnas.2132126100
MESSINA E., ISOLATION AND EXPANSION OF ADULT CARDIAC
Attorney, Agent or Firm:
SAEGUSA, Eiji et al. (1-7-1, Doshomachi,, Chuo-ku, Osaka-sh, Osaka, JP)
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