Title:
POLYKETIDE SYNTHASE ENZYMES AND RECOMBINANT DNA CONSTRUCTS THEREFOR
Document Type and Number:
WIPO Patent Application WO2000020601
Kind Code:
A3
Abstract:
Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.
Inventors:
REEVES CHRISTOPHER (US)
CHU DANIEL (US)
KHOSLA CHAITAN (US)
SANTI DANIEL (US)
WU KAI (US)
CHU DANIEL (US)
KHOSLA CHAITAN (US)
SANTI DANIEL (US)
WU KAI (US)
Application Number:
PCT/US1999/022886
Publication Date:
October 26, 2000
Filing Date:
October 01, 1999
Export Citation:
Assignee:
KOSAN BIOSCIENCES INC (US)
REEVES CHRISTOPHER (US)
CHU DANIEL (US)
KHOSLA CHAITAN (US)
SANTI DANIEL (US)
WU KAI (US)
REEVES CHRISTOPHER (US)
CHU DANIEL (US)
KHOSLA CHAITAN (US)
SANTI DANIEL (US)
WU KAI (US)
International Classes:
A61K31/436; A61P25/00; A61P37/06; C07D498/18; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N9/10; C12N9/12; C12N15/09; C12N15/52; C12P17/18; C12P19/32; C12P19/62; (IPC1-7): C12N15/52; C12N15/54; C12N15/62; C12N9/10; C12P17/18; C12P19/32; C07D498/18
Foreign References:
| EP0323042A1 | 1989-07-05 | |||
| EP0356399A2 | 1990-02-28 | |||
| EP0463690A1 | 1992-01-02 |
Other References:
MOTAMEDI H ET AL.: "The biosynthetic gene cluster for the macrolactone ring of the immunosuppressant FK506", EUR. J. BIOCHEM., vol. 256, no. 3, 15 September 1998 (1998-09-15), pages 528 - 534, XP000906738
STASSI D L ET AL.: "Ethyl-substituted erythromycin derivatives produced by directed metabolic engineering", PROC. NATL. ACAD. SCI. USA, vol. 95, no. 13, 23 June 1998 (1998-06-23), pages 7305 - 7309, XP002143632
REYNOLDS K A ET AL.: "Rapamycin, FK506, and ascomycin -related compounds", DRUGS PHARM. SCI., vol. 82, 1997, pages 497 - 520, XP000906777
DUMONT F J ET AL.: "The immunosuppressive and toxic effects of FK-506 are mechanistically related: pharmacology of a novel antagonist of FK-506 and rapamycin", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 176, no. 3, 1 September 1992 (1992-09-01), pages 751 - 760, XP000906781
KAWAI M ET AL.: "Structure-activity profiles of macrolactam immunosuppressant FK-506 analogues", FEBS LETTERS, vol. 316, no. 2, January 1993 (1993-01-01), pages 107 - 113, XP002143633
MOTAMEDI H ET AL.: "Characterization of methyltransferase and hydroxylase genes involved in the biosynthesis of the immunosupressants FK506 and FK520", J. BACTERIOLOGY, vol. 178, no. 17, July 1996 (1996-07-01), pages 5243 - 5248, XP002137077
MOTAMEDI H ET AL.: "Structural organization of a multifunctional polyketide synthase involved in the biosynthesis of the macrolide immunosuppressant FK506", EUR. J. BIOCHEM., vol. 244, no. 1, 15 February 1997 (1997-02-15), pages 74 - 80, XP000906743
CHEN T S ET AL.: "Microbial transformation of immunosuppressive compounds. II. Specific desmethylation of 13-methoxy group of FK 506 and FR 9500520 by Actinomycete sp. ATCC 53828", J. ANTIBIOT., vol. 45, no. 4, April 1992 (1992-04-01), pages 577 - 580, XP002143634
SHAFIEE A ET AL.: "Enzymatic synthesis and immunosuppressive activity of novel desmethylated immunomycins (ascomycins)", J. ANTIBIOT., vol. 46, no. 9, September 1993 (1993-09-01), pages 1397 - 1405, XP002143635
KHOSLA C: "Harnessing the biosynthetic potential of modular polyketide synthases", CHEMICAL REVIEWS, vol. 97, no. 7, 1997, pages 2577 - 2590, XP002130646, ISSN: 0009-2665
STASSI D L ET AL.: "Ethyl-substituted erythromycin derivatives produced by directed metabolic engineering", PROC. NATL. ACAD. SCI. USA, vol. 95, no. 13, 23 June 1998 (1998-06-23), pages 7305 - 7309, XP002143632
REYNOLDS K A ET AL.: "Rapamycin, FK506, and ascomycin -related compounds", DRUGS PHARM. SCI., vol. 82, 1997, pages 497 - 520, XP000906777
DUMONT F J ET AL.: "The immunosuppressive and toxic effects of FK-506 are mechanistically related: pharmacology of a novel antagonist of FK-506 and rapamycin", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 176, no. 3, 1 September 1992 (1992-09-01), pages 751 - 760, XP000906781
KAWAI M ET AL.: "Structure-activity profiles of macrolactam immunosuppressant FK-506 analogues", FEBS LETTERS, vol. 316, no. 2, January 1993 (1993-01-01), pages 107 - 113, XP002143633
MOTAMEDI H ET AL.: "Characterization of methyltransferase and hydroxylase genes involved in the biosynthesis of the immunosupressants FK506 and FK520", J. BACTERIOLOGY, vol. 178, no. 17, July 1996 (1996-07-01), pages 5243 - 5248, XP002137077
MOTAMEDI H ET AL.: "Structural organization of a multifunctional polyketide synthase involved in the biosynthesis of the macrolide immunosuppressant FK506", EUR. J. BIOCHEM., vol. 244, no. 1, 15 February 1997 (1997-02-15), pages 74 - 80, XP000906743
CHEN T S ET AL.: "Microbial transformation of immunosuppressive compounds. II. Specific desmethylation of 13-methoxy group of FK 506 and FR 9500520 by Actinomycete sp. ATCC 53828", J. ANTIBIOT., vol. 45, no. 4, April 1992 (1992-04-01), pages 577 - 580, XP002143634
SHAFIEE A ET AL.: "Enzymatic synthesis and immunosuppressive activity of novel desmethylated immunomycins (ascomycins)", J. ANTIBIOT., vol. 46, no. 9, September 1993 (1993-09-01), pages 1397 - 1405, XP002143635
KHOSLA C: "Harnessing the biosynthetic potential of modular polyketide synthases", CHEMICAL REVIEWS, vol. 97, no. 7, 1997, pages 2577 - 2590, XP002130646, ISSN: 0009-2665
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