Title:
POLYMER MICROSPHERES AS ADJUVANTS IN THE PRODUCTION OF VACCINES AGAINST SCUTICOCILIATE FISH PARASITES
Document Type and Number:
WIPO Patent Application WO/2012/156564
Kind Code:
A3
Abstract:
The invention relates to the use of microspheres as adjuvants in the production of a vaccine against scuticociliatosis. The invention provides a vaccine containing spherical chitosan microspheres and a copolymer of methyl vinyl ether and maleic anhydride, that act as an adjuvant, the microspheres being covered with an antigen of a membrane of a pathogenic turbot parasite, the scutiociliate Philasterides dicentrarchi (synonym of Miamiensis avidus). The vaccine can induce powerful immune responses and an increased protection in the turbot against scuticociliatosis, when it is administered parenterally, improving the protection induced by vaccines using oleaginous adjuvants containing metabolisable oils, but without generating unwanted side effects in the fish.
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Inventors:
LEIRO VIDAL JOSE MANUEL (ES)
LUZARDO ALVAREZ ASTERIA (ES)
LAMAS FERNANDEZ JESUS (ES)
LEON RODRIGUEZ LAURA (ES)
BLANCO MENDEZ JOSE (ES)
LUZARDO ALVAREZ ASTERIA (ES)
LAMAS FERNANDEZ JESUS (ES)
LEON RODRIGUEZ LAURA (ES)
BLANCO MENDEZ JOSE (ES)
Application Number:
PCT/ES2012/070345
Publication Date:
April 11, 2013
Filing Date:
May 15, 2012
Export Citation:
Assignee:
UNIV SANTIAGO COMPOSTELA (ES)
LEIRO VIDAL JOSE MANUEL (ES)
LUZARDO ALVAREZ ASTERIA (ES)
LAMAS FERNANDEZ JESUS (ES)
LEON RODRIGUEZ LAURA (ES)
BLANCO MENDEZ JOSE (ES)
LEIRO VIDAL JOSE MANUEL (ES)
LUZARDO ALVAREZ ASTERIA (ES)
LAMAS FERNANDEZ JESUS (ES)
LEON RODRIGUEZ LAURA (ES)
BLANCO MENDEZ JOSE (ES)
International Classes:
A61K39/39; A61P33/00
Foreign References:
| EP1752141A1 | 2007-02-14 |
Other References:
CERCHIARA, T. ET AL.: "Chitosan and poly(methyl vinyl ether-comaleic anhydride) microparticles as nasal sustained delivery systems.", EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS., vol. 61, 2005, pages 196 - 198, XP027805092
LEON-RODRIGUEZ, L. ET AL.: "Incorporation of PVMMA to PLGA MS enhances lectin grafting and their in vitro activity in macrophages.", INTERNATIONAL JOURNALS OF PHARMACEUTICS., vol. 402, 8 October 2010 (2010-10-08), pages 165 - 174, XP055036180
PARAMA, A. ET AL.: "In vitro efficacy of glutaraldehyde crosslinked chitosan microspheres against fish-phatogenic ciliate Philasterides dicentrarchi", DISEASE OF AQUATIC ORGANISMS., vol. 64, 18 April 2005 (2005-04-18), pages 151 - 158, XP055140013
OLIVEIRA, B.F. ET AL.: "Spray-dried chitosan microspheres cross-linked with d,l-glyceraldehyde as a potential drug delivery system: preparation and characterization", BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, vol. 22, no. 3., September 2005 (2005-09-01), pages 353 - 360, XP055140014
FISCHER, S. ET AL.: "Chitosan Coated Plga-Microspheres-A modular System for Targeted Drug Delivery", EUROPEAN CELLS AND MATERIALS., vol. 7, 2004, pages 11 - 12, XP055140015
LAMAS, J. ET AL.: "Optimization of an inactivated Vaccine against a scuticociliate parasite of turbot: Effect of antigen, formalin and adjuvant concentration on antibody response and protection against the pathogen.", AQUACULTURE., vol. 278, no. 1-4, 10 March 2008 (2008-03-10), pages 22 - 26, XP022669394
See also references of EP 2711022A4
LEON-RODRIGUEZ, L. ET AL.: "Incorporation of PVMMA to PLGA MS enhances lectin grafting and their in vitro activity in macrophages.", INTERNATIONAL JOURNALS OF PHARMACEUTICS., vol. 402, 8 October 2010 (2010-10-08), pages 165 - 174, XP055036180
PARAMA, A. ET AL.: "In vitro efficacy of glutaraldehyde crosslinked chitosan microspheres against fish-phatogenic ciliate Philasterides dicentrarchi", DISEASE OF AQUATIC ORGANISMS., vol. 64, 18 April 2005 (2005-04-18), pages 151 - 158, XP055140013
OLIVEIRA, B.F. ET AL.: "Spray-dried chitosan microspheres cross-linked with d,l-glyceraldehyde as a potential drug delivery system: preparation and characterization", BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, vol. 22, no. 3., September 2005 (2005-09-01), pages 353 - 360, XP055140014
FISCHER, S. ET AL.: "Chitosan Coated Plga-Microspheres-A modular System for Targeted Drug Delivery", EUROPEAN CELLS AND MATERIALS., vol. 7, 2004, pages 11 - 12, XP055140015
LAMAS, J. ET AL.: "Optimization of an inactivated Vaccine against a scuticociliate parasite of turbot: Effect of antigen, formalin and adjuvant concentration on antibody response and protection against the pathogen.", AQUACULTURE., vol. 278, no. 1-4, 10 March 2008 (2008-03-10), pages 22 - 26, XP022669394
See also references of EP 2711022A4
Attorney, Agent or Firm:
ACUÑA ÁLVAREZ, Laura (Transferencia y EmprendimientoEdificio EMPRENDI, Campus Vida Santiago de Compostela, ES)
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