Title:
POLYMERASE CHAIN TRANSCRIPTION (PCT): EXPONENTIAL SYNTHESIS OF RNA AND MODIFIED RNA
Document Type and Number:
WIPO Patent Application WO/2019/014359
Kind Code:
A3
Abstract:
Provided herein are methods of generating a RNA, comprising: providing a reaction mixture comprising a DNA template, a forward primer, a reverse primer, a SFM4-3 polymerase, and ribonucleotides; and generating the RNA by thermocycling the reaction mixture. Also provided herein are methods for generating a RNA or modified RNA at an elevated temperature, the method comprising: contacting a reaction mixture comprising a DNA template, a primer, a SFM4-3 polymerase, and ribonucleoside triphosphates; and generating the RNA by incubation at the elevated temperature or putting the reaction mixture through repeated cycles of heating and cooling. Further provided herein are kits for transcribing, synthesizing, and/or amplifying a RNA sequence comprising: a polymerase; and ribonucleoside triphosphates.
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Inventors:
ROMESBERG FLOYD (US)
CHEN TINGJIAN (US)
CHEN TINGJIAN (US)
Application Number:
PCT/US2018/041660
Publication Date:
February 21, 2019
Filing Date:
July 11, 2018
Export Citation:
Assignee:
SCRIPPS RESEARCH INST (US)
International Classes:
C07H21/02; C12N15/66; C12P19/34; C12Q1/68
Domestic Patent References:
| WO2007025195A1 | 2007-03-01 | |||
| WO2006039656A2 | 2006-04-13 |
Foreign References:
| US20110098461A1 | 2011-04-28 | |||
| US20060003353A1 | 2006-01-05 | |||
| US20070219148A1 | 2007-09-20 | |||
| US20060141467A1 | 2006-06-29 | |||
| US20140093938A1 | 2014-04-03 | |||
| US5916777A | 1999-06-29 | |||
| US5939292A | 1999-08-17 |
Other References:
CHEN ET AL.: "Evolution of Thermophilic DNA Polymerases for the Recognition and Amplification of C2'-Modified DNA", NATURE CHEMISTRY, vol. 8, no. 6, June 2016 (2016-06-01), pages 1 - 18, XP055571086
CHEN ET AL.: "Polymerase Chain Transcription: Exponential Synthesis of RNA and Modified RNA", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 139, no. 29, 26 July 2017 (2017-07-26), pages 9949 - 9954, XP055571087
CHEN ET AL.: "Polymerase Chain Transcription: Exponential Synthesis of RNA and Modified RNA", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 139, no. 29, 26 July 2017 (2017-07-26), pages 9949 - 9954, XP055571087
Attorney, Agent or Firm:
PHUKAN, Priti (US)
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