Title:
POLYMERS, AND THEIR USE AS GELS FOR ELECTROPHORESIS
Document Type and Number:
WIPO Patent Application WO1988009981
Kind Code:
A3
Abstract:
PCT No. PCT/EP88/00515 Sec. 371 Date Jan. 31, 1989 Sec. 102(e) Date Jan. 31, 1989 PCT Filed Jun. 10, 1988 PCT Pub. No. WO88/09981 PCT Pub. Date Dec. 15, 1988.A novel synthesis of N-acryloyl-tris-(hydroxymethyl)aminomethane (NAT) is described. The polymerization kinetics and transparency of the poly(NAT) gels crosslinked by a crosslinker (CL) e.g. N,N'-methylenebisacrylamide (Bis), i.e. poly(NAT+Bis) polymer, are shown. Poly(NAT+Bis) gradient (4-24%) gels were prepared and found to resolve native proteins according to their size. The exclusion limit of these gels is over 3x106 Da. This is more than threefold higher than the exclusion limit of the polyacrylamide gradient gel of the same concentration. Therefore poly(NAT+CL) gels are better than polyacrylamide gels for the resolution of large proteins. It was demonstrated that poly(NAT+CL)-polyacrylamide composite gels could be prepared. The poly(NAT+CL) gels were found to be advantageous also for isoelectric focussing in carrier ampholytes or immobilized pH gradients. Poly(NAT+CL) gels are also very good for separation of nucleic acids. The resolution is particularly good in the range between several hundred to 4-5000 base pairs. Under the optimized conditions, DNA molecules of more than 20 kbases can be perfectly resolved.
Inventors:
KOZULIC BRANKO (CH)
MOSBACH KLAUS (CH)
MOSBACH KLAUS (CH)
Application Number:
PCT/EP1988/000515
Publication Date:
February 09, 1989
Filing Date:
June 10, 1988
Export Citation:
Assignee:
KOZULIC BRANKO (CH)
MOSBACH KLAUS (CH)
MOSBACH KLAUS (CH)
International Classes:
B01D57/02; C07C233/16; C08F20/52; C08F20/58; C08F20/60; C08F220/58; C08L33/04; C08L33/14; G01N27/26; G01N27/447; (IPC1-7): C07K3/14; G01N27/26; C07C103/60
Foreign References:
| US0419784A | 1890-01-21 | |||
| EP0040124A1 | 1981-11-18 | |||
| DE2159501A1 | 1972-06-22 |
Other References:
Analytical Biochemistry, volume 163, 1987, Academic Press, Inc., M. Kozulic et al.: "Poly-N-acryloyl-tris gels as anticonvection media for electrophoresis and isoelectric focusing", pages 506-512
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