Title:
POLYNUCLEOTIDE CAUSING RNA INTERFERE AND METHOD OF REGULATING GENE EXPRESSION WITH THE USE OF THE SAME
Document Type and Number:
WIPO Patent Application WO/2005/116204
Kind Code:
A1
Abstract:
It is intended to provide a polynucleotide which exerts a high RNA interfere effect on a target gene but has little risk of causing RNA interfere on genes not relating to the target gene. From the base sequence of a target gene of RNA interfere, a sequence part following the rules (a) to (d) is detected and, based on the detection results, a polynucleotide causing RNAi is designed, synthesized and so on. (a) The 3’-terminal base is adenine, thymine or uracil. (b) The 5’-terminal base is guanine or cytosine. (c) The 3’-terminal sequence consisting of seven bases is rich in one or more bases selected from the group consistingof adenine, thymine and uracil. (d) The base number is at such a level as causing RNA interfere without expressing cytotoxicity.
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Inventors:
NAITO YUKI (JP)
FUJINO MASATO (JP)
OGUCHI SHINOBU (JP)
NATORI YUKIKAZU (JP)
FUJINO MASATO (JP)
OGUCHI SHINOBU (JP)
NATORI YUKIKAZU (JP)
Application Number:
PCT/IB2005/001647
Publication Date:
December 08, 2005
Filing Date:
May 11, 2005
Export Citation:
Assignee:
RNAI CO LTD (JP)
NAITO YUKI (JP)
FUJINO MASATO (JP)
OGUCHI SHINOBU (JP)
NATORI YUKIKAZU (JP)
NAITO YUKI (JP)
FUJINO MASATO (JP)
OGUCHI SHINOBU (JP)
NATORI YUKIKAZU (JP)
International Classes:
A61K31/713; A61P35/00; C12N15/09; C12N15/11; A61K48/00; G06F17/30; G06F17/40; G06F17/50; (IPC1-7): C12N15/09; A61K31/713; A61K48/00; A61P35/00
Domestic Patent References:
| WO2004048566A1 | 2004-06-10 |
Other References:
UI-TEI K. ET AL.: "Guidelines for the selection og highly effective siRNA sequences for mammalian and chick RNA interference.", NUCLEIC ACIDS RES., vol. 32, no. 3, 9 February 2004 (2004-02-09), pages 936 - 948, XP002329955
UI-TEI K. ET AL.: "RNAi Koka no Takai siRNA Hairetsu Biology Society of Japan Nenkai Program Koen Yoshishu.", DAI 27 KAI THE MOLECULAR BIOLOGY SOCIETY OF JAPAN NENKAI PROGRAM KOEN YOSHISHU., 25 November 2004 (2004-11-25), pages 487, XP002997047
NAITO Y. ET AL.: "siDirect : highly effective target-specific siRNA design software for mammalian RNA interference.", NUCLEIC ACIDS RES, vol. 32, 1 July 2004 (2004-07-01), pages W124 - W129, XP002329956
NAITO Y. ET AL.: "dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.", NUCLEIC ACIDS RES., vol. 33, 1 July 2005 (2005-07-01), pages W589 - W591, XP002997048
DOI Z. ET AL.: "Short-interfering-RNA-mediated gene silencing in mammalian Dicer and eIF2C translation initiation factors.", CURR.BIOL., vol. 13, no. 1, 2003, pages 41 - 46, XP002997049
See also references of EP 1752536A4
UI-TEI K. ET AL.: "RNAi Koka no Takai siRNA Hairetsu Biology Society of Japan Nenkai Program Koen Yoshishu.", DAI 27 KAI THE MOLECULAR BIOLOGY SOCIETY OF JAPAN NENKAI PROGRAM KOEN YOSHISHU., 25 November 2004 (2004-11-25), pages 487, XP002997047
NAITO Y. ET AL.: "siDirect : highly effective target-specific siRNA design software for mammalian RNA interference.", NUCLEIC ACIDS RES, vol. 32, 1 July 2004 (2004-07-01), pages W124 - W129, XP002329956
NAITO Y. ET AL.: "dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.", NUCLEIC ACIDS RES., vol. 33, 1 July 2005 (2005-07-01), pages W589 - W591, XP002997048
DOI Z. ET AL.: "Short-interfering-RNA-mediated gene silencing in mammalian Dicer and eIF2C translation initiation factors.", CURR.BIOL., vol. 13, no. 1, 2003, pages 41 - 46, XP002997049
See also references of EP 1752536A4
Attorney, Agent or Firm:
Shamoto, Ichio (Section 206 New Ohtemachi Bldg., 2-1,
Ohtemachi 2-chom, Chiyoda-ku Tokyo, JP)
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