Title:
POLYNUCLEOTIDE FOR CELL SURFACE LAYER EXPRESSION
Document Type and Number:
WIPO Patent Application WO/2014/157141
Kind Code:
A1
Abstract:
The present invention discloses a polynucleotide for cell surface layer expression. This polynucleotide comprises a promoter, a secretion signal sequence, a sequence for encoding a target protein, and a sequence for encoding the protein localized to the cell surface layer or the cell membrane binding region thereof, and the promoter is a promoter of a gene encoding the protein localized to the cell surface layer. Provided is a polynucleotide for cell surface layer expression that enables production of yeast containing a highly active enzyme that is present in the cell surface layer.
Inventors:
KONDO AKIHIKO (JP)
HASUNUMA TOMOHISA (JP)
INOKUMA KENTARO (JP)
HASUNUMA TOMOHISA (JP)
INOKUMA KENTARO (JP)
Application Number:
PCT/JP2014/058189
Publication Date:
October 02, 2014
Filing Date:
March 25, 2014
Export Citation:
Assignee:
NAT UNIV CORP UNIV KOBE (JP)
International Classes:
C12N15/09; C12N1/19; C12P7/06
Foreign References:
| JP2008086310A | 2008-04-17 | |||
| JP2005245335A | 2005-09-15 |
Other References:
RAM, A. F. J. ET AL.: "Green fluorescent protein- cell wall fusion proteins are covalently incorporated into the cell wall of Saccharomyces cerevisiae", FEMS MICROBIOLOGY LETTERS, vol. 162, 1998, pages 249 - 255
KOTAKA, A. ET AL.: "Enhancement of beta- glucosidase activity on the cell -surface of sake yeast by disruption of SED1", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 109, no. 5, 2010, pages 442 - 446
WHEELER, R. T. ET AL.: "A Saccharomyces cerevisiae mutant with increased virulence", PROC. NATL. ACAD. SCI. USA, vol. 100, no. 5, 2003, pages 2766 - 2770
SU , G.-D. ET AL.: "Surface display of active lipase in Pichia pastoris using Sed1 as an anchor protein", BIOTECHNOLOGY LETTERS, vol. 32, 2010, pages 1131 - 1136
INOKUMA, K. ET AL.: "Efficient yeast cell - surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter", BIOTECHNOLOGY FOR BIOFUELS, vol. 7, 14 January 2014 (2014-01-14), pages 8, Retrieved from the Internet [retrieved on 20140612]
KOTAKA, A. ET AL.: "Enhancement of beta- glucosidase activity on the cell -surface of sake yeast by disruption of SED1", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 109, no. 5, 2010, pages 442 - 446
WHEELER, R. T. ET AL.: "A Saccharomyces cerevisiae mutant with increased virulence", PROC. NATL. ACAD. SCI. USA, vol. 100, no. 5, 2003, pages 2766 - 2770
SU , G.-D. ET AL.: "Surface display of active lipase in Pichia pastoris using Sed1 as an anchor protein", BIOTECHNOLOGY LETTERS, vol. 32, 2010, pages 1131 - 1136
INOKUMA, K. ET AL.: "Efficient yeast cell - surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter", BIOTECHNOLOGY FOR BIOFUELS, vol. 7, 14 January 2014 (2014-01-14), pages 8, Retrieved from the Internet
Attorney, Agent or Firm:
SHINDO, Takuya et al. (JP)
Takuya Shindo (JP)
Takuya Shindo (JP)
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