Title:
POLYPEPTIDE HAVING ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME 2, AND GENE ENCODING SAID POLYPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2018/159635
Kind Code:
A1
Abstract:
Provided is an enzyme having no sugar chain and having the activity of soluble angiotensin converting enzyme 2. Also provided are: a gene capable of mass production by a prokaryotic microorganism; an expression plasmid which carries the gene; and a transformant which is transformed with the expression plasmid. Provided are: an amino acid sequence for an enzyme that is derived from a prokaryotic microorganism, has no sugar chain and has the activity of soluble angiotensin converting enzyme 2; a nucleotide sequence for a gene for the enzyme; an expression plasmid which carries the gene; and a transformant which is transformed with the expression plasmid.
Inventors:
NIRASAWA SATORU (JP)
TAKAHASHI SAORI (JP)
TAKAHASHI SAORI (JP)
Application Number:
PCT/JP2018/007338
Publication Date:
September 07, 2018
Filing Date:
February 27, 2018
Export Citation:
Assignee:
JAPAN INTERNATIONAL RES CENTER FOR AGRICULTURAL SCIENCES (JP)
AKITA PREFECTURE (JP)
AKITA PREFECTURE (JP)
International Classes:
C12N15/57; C12N1/21; C12N9/48; C12N15/63
Other References:
"Paenibacillus sp. BIHB4019, complete genome", DATABASE DDBJ, vol. 1524, no. 3571, 5 August 2016 (2016-08-05), pages 1, Retrieved from the Internet [retrieved on 20180509]
DONOGHUE, MARY ET AL.: "A novel angiotensin- converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9", CIRCULATION RESEARCH, vol. 87, 2000, XP002207553
VICKERS, CHAD ET AL.: "Hydrolysis of biological peptides by human angiotensin-converting enzyme- related carboxypeptidase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 17, 26 April 2002 (2002-04-26), pages 14838 - 14843, XP055554415, Retrieved from the Internet
NIEMIROWICZ, GABRIELA ET AL.: "Two metallocarboxypeptidases from the protozoan Trypanosoma cruzi belong to the M32 family, found so far only in prokaryotes", BIOCHEMICAL JOURNAL, vol. 401, no. 2, 21 December 2006 (2006-12-21), pages 399 - 410, XP055554416, Retrieved from the Internet
DONOGHUE, MARY ET AL.: "A novel angiotensin- converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9", CIRCULATION RESEARCH, vol. 87, 2000, XP002207553
VICKERS, CHAD ET AL.: "Hydrolysis of biological peptides by human angiotensin-converting enzyme- related carboxypeptidase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 17, 26 April 2002 (2002-04-26), pages 14838 - 14843, XP055554415, Retrieved from the Internet
NIEMIROWICZ, GABRIELA ET AL.: "Two metallocarboxypeptidases from the protozoan Trypanosoma cruzi belong to the M32 family, found so far only in prokaryotes", BIOCHEMICAL JOURNAL, vol. 401, no. 2, 21 December 2006 (2006-12-21), pages 399 - 410, XP055554416, Retrieved from the Internet
Attorney, Agent or Firm:
HIRAYAMA Kazuyuki (JP)
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