Title:
POLYPEPTIDE IMPROVED IN PROTEIN PURITY AND AFFINITY FOR ANTIGEN, CONJUGATE THEREOF WITH ANTIBODY OR ANTIGEN-BINDING FRAGMENT, AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2018/194376
Kind Code:
A1
Abstract:
The present invention relates to a polypeptide improved in protein purity and in affinity for a target antigen, a conjugate thereof with an antibody or antigen-binding fragment, and a preparation method of the polypeptide and the conjugate. The polypeptide or the conjugate thereof according to the present invention does not undergo glycosylation even when produced in a eukaryotic cell and thus has high protein purity and affinity for a target antigen, showing a very high value as a reagent for diagnosis or treatment of a disease.
Inventors:
LEE JONG-HO (KR)
LEE HYUN-JONG (KR)
KO BONG-KOOK (KR)
KIM KYU-TAE (KR)
LEE JONG-SEO (KR)
LEE HYUN-JONG (KR)
KO BONG-KOOK (KR)
KIM KYU-TAE (KR)
LEE JONG-SEO (KR)
Application Number:
PCT/KR2018/004516
Publication Date:
October 25, 2018
Filing Date:
April 18, 2018
Export Citation:
Assignee:
ABCLON INC (KR)
International Classes:
C07K16/24; C12N15/11
Domestic Patent References:
| WO2009080811A1 | 2009-07-02 | |||
| WO1995019374A1 | 1995-07-20 | |||
| WO2009080811A1 | 2009-07-02 |
Foreign References:
| KR20170018888A | 2017-02-20 | |||
| KR20170049732A | 2017-05-11 |
Other References:
LINDBORG, MALIN: "High-affinity binding to staphylococcal protein A by an engineered dimeric Affibody molecule", PROTEIN ENGINEERING, DESIGN & SELECT ION, vol. 26, no. 10, 2013, pages 635 - 644, XP055505107, DOI: 10.1093/protein/gzt038
LENDEL, CHRISTOFER: "Structural Basis for Molecular Recognition in an Aff ibody:Affibody Complex", JOURNAL OF MOLECULAR BIOLOGY, vol. 359, no. 5, 23 June 2006 (2006-06-23), pages 1293 - 1304, XP024951135, ISSN: 0022-2836 , DOI: 10.1016/j.jmb.2006.04.043
TASHIRO, MITSURU: "Structures of bacterial immunoglobulin-binding domains and their complexes with immunoglobulins", CURRENT OPINION IN STRUCTURAL BI OLOGY, vol. 5, no. 4, 1995, pages 471 - 481, XP008133979, DOI: 10.1016/0959-440X(95)80031-X
YU , FEIFAN: "An affibody-adalimumab hybrid blocks combined IL -6 and TNF-t riggered serum amyloid A secretion in vivo", MABS, vol. 6, no. 6, 2014, pages 15 98 - 1607, XP055457021, DOI: 10.4161/mabs.36089
ORLOVA ET AL., CANCER RES., 2006
GRONWALL ET AL., J. BIOTECHNOL, 2007
GOLDSTEIN R ET AL., EXPERT REV ANTICANCER THER, 2013
YU F ET AL., MABS, 2014
WRIGHT A ET AL., EMBO, 1991
MERRIFIELD, J. AMER. CHEM. SOC., vol. 85, 1963, pages 2149 - 54
YANOFSKY, C., J. BACTERIOL., vol. 158, 1984, pages 1018 - 1024
"Remington's Pharmaceutical Sciences", 1995
HERSKOWITZ, I.HAGEN, D., ANN. REV. GENET., vol. 14, 1980, pages 399 - 445
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 584
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
NEEDLEMANWUNSCH, J. MOL. BIO., vol. 48, 1970, pages 443
LIPMAN, METHODS IN MOL. BIOL., vol. 24, 1988, pages 307 - 31
HIGGINSSHARP, GENE, vol. 73, 1988, pages 237 - 44
HIGGINSSHARP, CABIOS, vol. 5, 1989, pages 151 - 3
CORPET ET AL., NUC. ACIDS RES., vol. 16, 1988, pages 10881 - 90
HUANG ET AL., COMP. APPL. BIOSCI., vol. 8, 1992, pages 155 - 65
PEARSON ET AL., METH. MOL. BIOL., vol. 24, 1994, pages 307 - 31
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
COHEN, S.N. ET AL., PROC. NATL. ACAC. SCI. USA, vol. 9, 1973, pages 2110 - 2114
HANAHAN, D., J. MOL. BIOL., vol. 166, 1983, pages 557 - 580
DOWER, W.J. ET AL., NUCLEIC. ACIDS RES., vol. 16, 1988, pages 6127 - 6145
CAPECCHI, M.R., CELL, vol. 22, 1980, pages 479
GRAHAM, F.L. ET AL., VIROLOGY, vol. 52, 1973, pages 456
NEUMANN, E. ET AL., EMBO J., vol. 1, 1982, pages 841
WONG, T.K. ET AL., GENE, vol. 10, 1980, pages 87
GOPAL, MOL. CELL BIOL., vol. 5, 1985, pages 1188 - 1190
YANG ET AL., PROC. NATL. ACAD. SCI., vol. 87, 1990, pages 9568 - 9572
EISENHABER BEISENHABER F, METHODS MOL BIOL., 2010
See also references of EP 3613766A4
LENDEL, CHRISTOFER: "Structural Basis for Molecular Recognition in an Aff ibody:Affibody Complex", JOURNAL OF MOLECULAR BIOLOGY, vol. 359, no. 5, 23 June 2006 (2006-06-23), pages 1293 - 1304, XP024951135, ISSN: 0022-2836 , DOI: 10.1016/j.jmb.2006.04.043
TASHIRO, MITSURU: "Structures of bacterial immunoglobulin-binding domains and their complexes with immunoglobulins", CURRENT OPINION IN STRUCTURAL BI OLOGY, vol. 5, no. 4, 1995, pages 471 - 481, XP008133979, DOI: 10.1016/0959-440X(95)80031-X
YU , FEIFAN: "An affibody-adalimumab hybrid blocks combined IL -6 and TNF-t riggered serum amyloid A secretion in vivo", MABS, vol. 6, no. 6, 2014, pages 15 98 - 1607, XP055457021, DOI: 10.4161/mabs.36089
ORLOVA ET AL., CANCER RES., 2006
GRONWALL ET AL., J. BIOTECHNOL, 2007
GOLDSTEIN R ET AL., EXPERT REV ANTICANCER THER, 2013
YU F ET AL., MABS, 2014
WRIGHT A ET AL., EMBO, 1991
MERRIFIELD, J. AMER. CHEM. SOC., vol. 85, 1963, pages 2149 - 54
YANOFSKY, C., J. BACTERIOL., vol. 158, 1984, pages 1018 - 1024
"Remington's Pharmaceutical Sciences", 1995
HERSKOWITZ, I.HAGEN, D., ANN. REV. GENET., vol. 14, 1980, pages 399 - 445
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 584
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
NEEDLEMANWUNSCH, J. MOL. BIO., vol. 48, 1970, pages 443
LIPMAN, METHODS IN MOL. BIOL., vol. 24, 1988, pages 307 - 31
HIGGINSSHARP, GENE, vol. 73, 1988, pages 237 - 44
HIGGINSSHARP, CABIOS, vol. 5, 1989, pages 151 - 3
CORPET ET AL., NUC. ACIDS RES., vol. 16, 1988, pages 10881 - 90
HUANG ET AL., COMP. APPL. BIOSCI., vol. 8, 1992, pages 155 - 65
PEARSON ET AL., METH. MOL. BIOL., vol. 24, 1994, pages 307 - 31
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
COHEN, S.N. ET AL., PROC. NATL. ACAC. SCI. USA, vol. 9, 1973, pages 2110 - 2114
HANAHAN, D., J. MOL. BIOL., vol. 166, 1983, pages 557 - 580
DOWER, W.J. ET AL., NUCLEIC. ACIDS RES., vol. 16, 1988, pages 6127 - 6145
CAPECCHI, M.R., CELL, vol. 22, 1980, pages 479
GRAHAM, F.L. ET AL., VIROLOGY, vol. 52, 1973, pages 456
NEUMANN, E. ET AL., EMBO J., vol. 1, 1982, pages 841
WONG, T.K. ET AL., GENE, vol. 10, 1980, pages 87
GOPAL, MOL. CELL BIOL., vol. 5, 1985, pages 1188 - 1190
YANG ET AL., PROC. NATL. ACAD. SCI., vol. 87, 1990, pages 9568 - 9572
EISENHABER BEISENHABER F, METHODS MOL BIOL., 2010
See also references of EP 3613766A4
Attorney, Agent or Firm:
YOON, Dae Woong (KR)
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