Title:
POLYPEPTIDE AND METHOD FOR PRODUCING AMINO ACID USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/127752
Kind Code:
A1
Abstract:
A polypeptide that has a sequence having a sequence identity of 90% or more with respect to a sequence obtained by modifying one amino acid residue in the amino acid sequence represented by SEQ ID NO: 1, and that has a catalytic activity for a reductive amination reaction between one or more of compounds A represented by formula (1) or a salt thereof and one or more of compounds B represented by formula (2) or a salt thereof, or for an intramolecular reductive amination reaction of one or more of the compounds B or a salt thereof, wherein the catalytic activity is higher than that of a polypeptide having the amino acid sequence represented by SEQ ID NO: 1 under at least one reaction condition.
Inventors:
SHINODA KIYOMICHI (JP)
OHTA ATSUSHI (JP)
SHIINA JUNICHI (JP)
MATSUO ATSUSHI (JP)
ISHINO SOU (SG)
EJIMA HIROTAKA (JP)
ISHIYAMA SHIORI (JP)
NAKANO KAZUHIKO (JP)
TSUKADA KEISUKE (JP)
OHTA ATSUSHI (JP)
SHIINA JUNICHI (JP)
MATSUO ATSUSHI (JP)
ISHINO SOU (SG)
EJIMA HIROTAKA (JP)
ISHIYAMA SHIORI (JP)
NAKANO KAZUHIKO (JP)
TSUKADA KEISUKE (JP)
Application Number:
PCT/JP2022/047741
Publication Date:
July 06, 2023
Filing Date:
December 23, 2022
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
International Classes:
C12N9/06; C07C227/28; C07C229/12; C07K1/00; C07K1/02; C12N15/31; C12N15/53; C12P13/04; C12P21/00
Domestic Patent References:
| WO2003072770A1 | 2003-09-04 |
Foreign References:
| JP2005095167A | 2005-04-14 |
Other References:
MINDT MELANIE, HANNIBAL SILVIN, HEUSER MARIA, RISSE JOE MAX, SASIKUMAR KEERTHI, NAMPOOTHIRI K. MADHAVAN, WENDISCH VOLKER F.: "Fermentative Production of N-Alkylated Glycine Derivatives by Recombinant Corynebacterium glutamicum Using a Mutant of Imine Reductase DpkA From Pseudomonas putida", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 7, XP093041951, DOI: 10.3389/fbioe.2019.00232
KERBS ANASTASIA, MINDT MELANIE, SCHWARDMANN LYNN, WENDISCH VOLKER F.: "Sustainable Production of N-methylphenylalanine by Reductive Methylamination of Phenylpyruvate Using Engineered Corynebacterium glutamicum", MICROORGANISMS, vol. 9, no. 4, pages 824, XP093041754, DOI: 10.3390/microorganisms9040824
KERBS ANASTASIA, MINDT MELANIE, SCHWARDMANN LYNN, WENDISCH VOLKER F.: "Sustainable Production of N-methylphenylalanine by Reductive Methylamination of Phenylpyruvate Using Engineered Corynebacterium glutamicum", MICROORGANISMS, vol. 9, no. 4, pages 824, XP093041754, DOI: 10.3390/microorganisms9040824
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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