Title:
POLYPEPTIDE MODIFICATION METHOD FOR PURIFYING POLYPEPTIDE MULTIMERS
Document Type and Number:
WIPO Patent Application WO/2011/078332
Kind Code:
A1
Abstract:
Disclosed is a method for purifying or producing, by modifying the binding strength to Protein A, a multispecific antibody having binding activity for at least two kinds of antigens, wherein said multispecific antibody is purified or produced at a high purity and efficiency with only a Protein A purification step. The disclosed method for purifying or producing a multispecific antibody is characterized by modifying the amino acid residue of the antibody heavy chain constant regions and/or the antibody heavy chain variable regions. By introducing such amino acid modifications into the antibody, a multispecific antibody which has a modified binding strength to Protein A and has blood plasma retentivity greater than or equal to that of human IgG1 can be acquired at a high purity and efficiency.
Inventors:
IGAWA TOMOYUKI (JP)
SAMPEI ZENJIRO (JP)
WAKABAYASHI TETSUYA (JP)
ITO ERIKO (JP)
SAMPEI ZENJIRO (JP)
WAKABAYASHI TETSUYA (JP)
ITO ERIKO (JP)
Application Number:
PCT/JP2010/073361
Publication Date:
June 30, 2011
Filing Date:
December 24, 2010
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
IGAWA TOMOYUKI (JP)
SAMPEI ZENJIRO (JP)
WAKABAYASHI TETSUYA (JP)
ITO ERIKO (JP)
IGAWA TOMOYUKI (JP)
SAMPEI ZENJIRO (JP)
WAKABAYASHI TETSUYA (JP)
ITO ERIKO (JP)
International Classes:
C12N15/00; A61K39/395; C07K1/22; C07K16/46; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09
Domestic Patent References:
| WO2008090960A1 | 2008-07-31 | |||
| WO2007114325A1 | 2007-10-11 | |||
| WO2006106905A1 | 2006-10-12 |
Foreign References:
| JPH09506001A | 1997-06-17 |
Other References:
LINDHOFER, HORST ET AL.: "Preferential species-restricted heavy/light chain pairing in rat/mouse quadromas. Implications for a single- step purification of bispecific antibodies.", J. IMMUNOL., vol. 155, no. 1, 1995, pages 219 - 225, XP002190775
KIM, JIN-KYOO ET AL.: "Mapping the site on human IgG for binding of the MHC class I-related receptor, FcRn", EUR. J. IMMUNOL., vol. 29, 1999, pages 2819 - 2825, XP002300286
See also references of EP 2522724A4
KIM, JIN-KYOO ET AL.: "Mapping the site on human IgG for binding of the MHC class I-related receptor, FcRn", EUR. J. IMMUNOL., vol. 29, 1999, pages 2819 - 2825, XP002300286
See also references of EP 2522724A4
Attorney, Agent or Firm:
SHIMIZU, Hatsushi et al. (JP)
Spring water Original aim (JP)
Spring water Original aim (JP)
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