Title:
POLYVINYL ALCOHOL-DEGRADING ENZYME AND PRODUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2018/159497
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a novel PVA-degrading enzyme, the identity of which is revealed at the amino acid sequence level; a production method therefor; DNA coding for the enzyme; and recombinant DNA and a transformant which contain the DNA. The problem is solved by providing a polyvinyl alcohol-degrading enzyme having features (1) to (3), a production method therefor, DNA coding for the enzyme, and recombinant DNA and a transformant which contain the DNA having: (1) an activity of oxidizing polyvinyl alcohol and producing hydrogen peroxide; (2) an activity of hydrolyzing a β-diketone; and (3) a molecular weight of 100,000 ± 20,000 as determined through SDS-polyacrylamide gel electrophoresis.
Inventors:
YAMANAKA AKIHIRO (JP)
MATSUO NAOKI (JP)
MORI TETSUYA (JP)
NISHIMOTO TOMOYUKI (JP)
MATSUO NAOKI (JP)
MORI TETSUYA (JP)
NISHIMOTO TOMOYUKI (JP)
Application Number:
PCT/JP2018/006767
Publication Date:
September 07, 2018
Filing Date:
February 23, 2018
Export Citation:
Assignee:
HAYASHIBARA CO (JP)
International Classes:
C12N9/04; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/14; C12N15/09; C12P21/02
Foreign References:
| JPH09206079A | 1997-08-12 | |||
| JPH11103861A | 1999-04-20 | |||
| JP2004000259A | 2004-01-08 | |||
| JP2005278639A | 2005-10-13 | |||
| JP2006042611A | 2006-02-16 | |||
| JP2006042612A | 2006-02-16 | |||
| JP2006180706A | 2006-07-13 | |||
| JP5891478B2 | 2016-03-23 | |||
| JPH09206079A | 1997-08-12 |
Other References:
KAWAI, F. ET AL.: "Biochemistry of microbial polyvinyl alcohol degradation", APPL MICROBIOL BIOTECHNOL, vol. 84, 2009, pages 227 - 237, XP019737779
SHIMAO, M. ET AL.: "The gene pvaB encodes oxidized polyvinylalcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA", MICROBIOLOGY, vol. 146, 2000, pages 649 - 657, XP055561061
KLOMKLANG, W. ET AL.: "Biochemical and molecular characterization of a periplasmic hydro lase for oxidized polyvinyl alcohol from Sphingomonas sp. strain 113P3", MICROBIOLOGY, vol. 151, 2005, pages 1255 - 1262, XP055561107
SHIMAO, M. ET AL.: "Properties and Roles of Bacterial Symbionts of Polyvinyl Alcohol-Utilizing Mixed Cultures", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 46, no. 3, 1983, pages 605 - 610, XP055561137
MATSUMURA ET AL., MACROMOLECULES, vol. 32, 1999, pages 7753 - 7761
MORITA ET AL., AGRIC. BIOL. CHEM., vol. 43, 1979, pages 1225 - 1235
SAKAI ET AL., AGRIC. BIOL. CHEM., vol. 45, 1981, pages 63 - 71
SAKAI ET AL., AGRIC. BIOL. CHEM., vol. 47, 1983, pages 153 - 155
SHIMAO ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 60, 1996, pages 1056 - 1062
YANG ET AL., CHEMBIOCHEM., vol. 15, 2014, pages 1882 - 1886
See also references of EP 3581647A4
SHIMAO, M. ET AL.: "The gene pvaB encodes oxidized polyvinylalcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA", MICROBIOLOGY, vol. 146, 2000, pages 649 - 657, XP055561061
KLOMKLANG, W. ET AL.: "Biochemical and molecular characterization of a periplasmic hydro lase for oxidized polyvinyl alcohol from Sphingomonas sp. strain 113P3", MICROBIOLOGY, vol. 151, 2005, pages 1255 - 1262, XP055561107
SHIMAO, M. ET AL.: "Properties and Roles of Bacterial Symbionts of Polyvinyl Alcohol-Utilizing Mixed Cultures", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 46, no. 3, 1983, pages 605 - 610, XP055561137
MATSUMURA ET AL., MACROMOLECULES, vol. 32, 1999, pages 7753 - 7761
MORITA ET AL., AGRIC. BIOL. CHEM., vol. 43, 1979, pages 1225 - 1235
SAKAI ET AL., AGRIC. BIOL. CHEM., vol. 45, 1981, pages 63 - 71
SAKAI ET AL., AGRIC. BIOL. CHEM., vol. 47, 1983, pages 153 - 155
SHIMAO ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 60, 1996, pages 1056 - 1062
YANG ET AL., CHEMBIOCHEM., vol. 15, 2014, pages 1882 - 1886
See also references of EP 3581647A4
Attorney, Agent or Firm:
SUMA Mitsuo (JP)
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