Title:
POROUS STRUCTURE CONFINEMENT FOR CONVECTION SUPPRESSION
Document Type and Number:
WIPO Patent Application WO/2023/229982
Kind Code:
A3
Abstract:
Provided herein are technologies for reducing fluid convection during processing and analysis of a biological sample. The provided technologies can include the use of a porous insert to limit fluid convection adjacent to a biological sample. In particular, provided technologies can include providing a substrate comprising a capture area, a biological sample comprising a cell disposed on the capture area, a buffer disposed on the biological sample, and a gasket disposed on the substrate, wherein the gasket provides a chamber comprising the lateral dimension of the capture area and includes a height above the capture area and biological sample; and a porous insert positioned in the chamber and in contact with the buffer and/or the biological sample, wherein the porous insert limits free flow space in the chamber, thereby reducing fluid convection adjacent to the biological sample.
Inventors:
HUNG LUNG-HSIN (US)
NAGENDRAN MONICA (US)
WHEELER TOBIAS DANIEL (US)
NAGENDRAN MONICA (US)
WHEELER TOBIAS DANIEL (US)
Application Number:
PCT/US2023/023074
Publication Date:
January 04, 2024
Filing Date:
May 22, 2023
Export Citation:
Assignee:
10X GENOMICS INC (US)
International Classes:
B01L3/00; C12Q1/24
Foreign References:
| US20180085751A1 | 2018-03-29 | |||
| US20150017709A1 | 2015-01-15 |
Attorney, Agent or Firm:
YAN, Tianran et al. (US)
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