Title:
PRAMYXOVIRUS VECTORS ENCODING ANTIBODY AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2003/102183
Kind Code:
A1
Abstract:
It is intended to provide a paramyxovirus vector expressing a polypeptide containing antibody variable regions. This vector, which encodes antibody H chain and L chain variable regions, expresses these antibody chains at the same time to form Fab. Also, a single-stranded antibody is successfully expressed at a high level. The above vector is appropriately usable as a gene therapeutic vector to be administered to a living body either in vivo or ex vivo. In particular, a vector expressing an antibody fragment against nerve elongation inhibitor is useful in treating nerve injury. The above vector expressing an antibody which inhibits immunopotentiation signal transfer enables the prolonged expression of a gene from the vector.
Inventors:
INOUE MAKOTO (JP)
HASEGAWA MAMORU (JP)
HIRONAKA TAKASHI (JP)
HASEGAWA MAMORU (JP)
HIRONAKA TAKASHI (JP)
Application Number:
PCT/JP2003/007005
Publication Date:
December 11, 2003
Filing Date:
June 03, 2003
Export Citation:
Assignee:
DNAVEC RESEARCH INC (JP)
INOUE MAKOTO (JP)
HASEGAWA MAMORU (JP)
HIRONAKA TAKASHI (JP)
INOUE MAKOTO (JP)
HASEGAWA MAMORU (JP)
HIRONAKA TAKASHI (JP)
International Classes:
A61K48/00; A61P19/08; A61P25/00; A61P37/06; A61P43/00; C07K16/00; C07K16/22; C07K16/28; C12N15/86; (IPC1-7): C12N15/09; C12N7/00; A61K35/76; A61K39/395; A61K48/00; A61P19/08; A61P25/00; A61P37/06; A61P43/00; C07K16/18; C07K16/28; C12P21/02
Other References:
LI HO ET AL.: "A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression", J. VIROL., vol. 74, no. 14, July 2000 (2000-07-01), pages 6564 - 6569, XP002931595
MASAKI I. ET AL.: "Angiogenic gene therapy for experimental critical limb ischemia: acceleration of limb loss by overexpression of vascular endothelial growth factor 165 but not of fiboblast growth factor-2", CIRC. RES., vol. 90, no. 9, 17 May 2002 (2002-05-17), pages 966 - 973, XP002972374
INOUYE R.T. ET AL.: "Potent inhibition of human immunodeficiency virus type 1 in primary T cells and alveolar macrophages by a combination anti-Rev strategy delivered in an adeno-associated virus vector", J. VIROL., vol. 71, no. 5, May 1997 (1997-05-01), pages 4071 - 4078, XP002972375
LIANG X.H. ET AL.: "Expression of a biologically active antiviral antibody using a sindbis virus vector system", MOL. IMMUNOL., vol. 34, no. 12-13, August 1997 (1997-08-01) - September 1997 (1997-09-01), pages 907 - 917, XP002972376
MORIMOTO K. ET AL.: "High level expression of a human rabies virus-neutralizing monoclonal antibody by a rhabdovirus-based vector", J. IMMUNOL. METHODS, vol. 252, no. 1-2, 1 June 2001 (2001-06-01), pages 199 - 206, XP002201100
BROSAMLE C. ET AL.: "Regeneration of lesioned corticospinal tract fibers in the adult rat induced by a recombinant, humanized IN-1 antibody fragment", J. NEUROSCI., vol. 20, no. 21, 1 November 2000 (2000-11-01), pages 8061 - 8068, XP002238449
YU X.Z. ET AL.: "CD28-specific antibody prevents graft-versus-host disease in mice", J. IMMUNOL., vol. 164, no. 9, 1 May 2000 (2000-05-01), pages 4564 - 4568, XP002972377
MASAKI I. ET AL.: "Angiogenic gene therapy for experimental critical limb ischemia: acceleration of limb loss by overexpression of vascular endothelial growth factor 165 but not of fiboblast growth factor-2", CIRC. RES., vol. 90, no. 9, 17 May 2002 (2002-05-17), pages 966 - 973, XP002972374
INOUYE R.T. ET AL.: "Potent inhibition of human immunodeficiency virus type 1 in primary T cells and alveolar macrophages by a combination anti-Rev strategy delivered in an adeno-associated virus vector", J. VIROL., vol. 71, no. 5, May 1997 (1997-05-01), pages 4071 - 4078, XP002972375
LIANG X.H. ET AL.: "Expression of a biologically active antiviral antibody using a sindbis virus vector system", MOL. IMMUNOL., vol. 34, no. 12-13, August 1997 (1997-08-01) - September 1997 (1997-09-01), pages 907 - 917, XP002972376
MORIMOTO K. ET AL.: "High level expression of a human rabies virus-neutralizing monoclonal antibody by a rhabdovirus-based vector", J. IMMUNOL. METHODS, vol. 252, no. 1-2, 1 June 2001 (2001-06-01), pages 199 - 206, XP002201100
BROSAMLE C. ET AL.: "Regeneration of lesioned corticospinal tract fibers in the adult rat induced by a recombinant, humanized IN-1 antibody fragment", J. NEUROSCI., vol. 20, no. 21, 1 November 2000 (2000-11-01), pages 8061 - 8068, XP002238449
YU X.Z. ET AL.: "CD28-specific antibody prevents graft-versus-host disease in mice", J. IMMUNOL., vol. 164, no. 9, 1 May 2000 (2000-05-01), pages 4564 - 4568, XP002972377
Attorney, Agent or Firm:
Shimizu, Hatsushi (1-1-1 Oroshi-mach, Tsuchiura-shi Ibaraki, JP)
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