Precision Fluid Dispensing System

BACKGROUND

Field of Invention

The invention relates generally to the field of precision fluid dispensing for Bioscience applications and more particularly to a two-piece pump with a multiple diameter cylinder dual piston and multiple inlet and outlet ports that can be controlled by a micro-controlled precision drive system capable of closed loop control.

Description of the Problem Solved

Syringe pumps that use glass syringes and pistons with seals are routinely used for fluid dispensing in the Biosciences. Independent valves are usually used to control fluid inlet and outlet functions. Currently, a syringe pump made by Cavro, Kloehn & Hamilton provides various syringe sizes for dispensing in the range of 1 microliter to 50 milliliter. Valve functions provide for multiple inlet and outlet ports. Although the syringe barrel plugs directly into the valve body, using seals, the valve can be essentially separate from the syringe. The syringe area and the piston linear displacement define the dispensed syringe fluid volume.

In most cases, a stepper motor that is coupled to a lead screw to translate the rotary to linear motion controls the syringe piston displacement. The stepper motors in high-end models have shaft encoders so as to provide for drive overload detection for motor step loss.

The Cavro XL 3000, for example, with 8-port distribution valve, provides for a linear resolution of either 3000 or 24000 steps or increments in its 60 mm available piston travel. An optical encoded stepper motor also controls the valve stack port positioning. The valve stack can be directly or indirectly coupled to a second stepper motor shaft, and the syringe output end can be inserted into the bottom of the valve stack utilizing a seal.

The Hamilton Microlab 500 fluid diluters and dispensers are also precision fluid measuring instruments based on syringe technology. The Hamilton systems often use two syringe pumps to accomplish diluter functions . Sample dilutions are made by first filling one of the syringes with a programmed amount of diluent from a reservoir followed by aspirating a programmed amount of sample into the end of the dispensing tube using the second syringe. The last step to accomplish the dilution is to dispense the sample and diluent into a vial . Dispensing functions using a two syringe pump Hamilton unit are accomplished by filling one syringe with reagent 1 and the other with reagent 2. The two syringe pumps output the desired ratio into a common tube for vial filling.

The syringe pumps are not known to provide reliability for long run cycles due to failure of the piston and cylinder seal and the seals that make up the valve stack. Also, cleaning of the system often requires the operator to completely disassemble the syringe cylinder and piston along with the rotary valve stack. This disables the entire dispensing system. In many applications, individuals completely flush out the dispenser with cleaning solutions rather than

dismantle the system.

A simple two-piece pump is known in the art and is usually provided in either stainless steel or ceramic materials. This type of pump consists of a piston and cylinder in which the piston can also provide the valving functions. SPC France, NeoCeram and others manufacture two-piece pumps for the pharmaceutical industry, and recently two diameter pumps providing smaller volume dispensing capability have also appeared on the market.

NeoCeram and others have also built pumps that have multiple ports. The pump does not require moving seals between the piston and cylinder as close tolerances and a fluid provide the sealing function. The piston with a valve slot can be rotated between predetermined positions to select either inlet or outlet ports. When the correct inlet or outlet port has been selected, the linear motion provides for fluid aspiration or dispensing. In special cases, to recover pump fluid at the end of dispensing or for using cleaning fluids, inlet and outlet ports can be aligned. In nearly all cases the two-piece pumps have been designed and developed for high-speed fluid filling manufacturing lines. The drive hardware is expensive requiring precision ground ball screws along with motor encoders . The motor encoders can only detect the motion of the motor and not that of other elements in the drive train to the pump piston.

Syringe type positive displacement pumps are capable of dispensing very small fluid quantities but when the volumes drop below 3 microliters, getting the drop off the tube or

nozzle requires contact or very near contact to the dispensing surface. Cartesian Technologies and others have provided active nozzles to simplify small volume delivery for the micro-array market. Cartesian Technologies uses a solenoid valve that is fluid coupled and synchronized to a syringe pump. Other systems use aerosol jet or piezoelectric devices coupled to syringe pumps to assist in small volume dispensing.

What is badly needed is a cost effective, small volume, easily cleanable, precision dispensing system for the Biosciences including a dual piston/cylinder multiple port pump that utilizes two pistons and two cylinders that can be coupled together making a single pump along with multiple inlet and outlet ports, and a precision pump drive system with cost effective electronics to meet these requirements. The pump drive needs to provide accurate dispensing with the position controlled by a linear measurement means. A controller can also provide capability for synchronization with active nozzles along with A/D capability to provide for external sensors to be read, such as a pressure transducer.

SUMMARY OF THE INVENTION

The present invention relates to a two-piece pump and a precision closed loop controller drive system to address the small volume precision dispensing requirements of the Bioscience market. The two-piece pump can contain a cylinder and piston with two different diameters to create a seal-less pump with integrated valving. The pump cylinder and piston should have more than two diameters or the diameters can be

tapered or curved. In a multiple diameter pump the amount of fluid dispensed is related to the difference of the diameter areas times the linear displacement of the piston.

The present invention, combines a multiple diameter pump with a pump having multiple inlet and outlet ports and with a precision control system. The configuration allows for precision multiple outlet dispenses in a single pump that can be used, for example, with microtiter plate pipetting. A positive displacement pump option for microtiter plate dispensing is the use of a pump with a multiple inlet and outlet ports. The preferred position of inlet ports on the multi-diameter cylinder is on the smaller diameter part of the cylinder, while the preferred position of outlet ports is on the larger diameter of the cylinder. However, it should be noted that the ports could be located anywhere on the cylinder and still be within the scope of the present invention. The smaller diameter part of the cylinder is usually located at the lower portion of the cylinder relative to the larger diameter portion. The piston can have a groove on the smaller diameter part connected to a groove on the larger diameter part. The number of inlet and outlet ports are limited by the piston/cylinder diameter and the spacing between adjacent ports. If 5 mm were used as a minimum spacing between ports, and the pump has (10) 1 mm ports, where 8 ports are outlet and 2 ports are inlets, the necessary pump diameter would be just over 19 mm in diameter. For 19 mm diameter pump to dispense in the microliter range, the difference in the diameters should be small and the linear drive capable of very small displacements .

One of the preferred pump configurations of the present invention can use two pistons and two cylinders, multiple ports with 2 inlet ports and 8 outlet ports. The pump is capable of mixing because it can aspirate fluid into the pump from port 1, and then from port 2, followed by rotating the piston to accomplish annular mixing. A piston groove can assist in the mixing, but the pump can have other features to assist in mixing as long as none of these features trap air during operation. For recovery of dispensing fluid in the pump the system could use (9) outlet ports where the 9 ^th port is aligned with the inlet port. The inlet port can be connected to the fluid supply or any other container for recovery. In this configuration the aligned outlet port can be connected to an air source which would force remaining fluid out the aligned inlet port. In another configuration, the aligned inlet and outlet port could be connected to a cleaning or flush solution. The pump piston groove could be cleaned by fluid pressure at the inlet port and the piston can be rotated to clean the fluid boundary layer between the piston and cylinder.

The two pistons can be connected so that the movement of the larger piston is directly coupled to the smaller piston. There is a small fluid gap between the two pistons. This same motion can be accomplished by moving the two cylinders while holding the pistons fixed relative to each other.

The smaller piston can be connected to the movement of the upper piston resulting in dispensing volumes related to the difference of the two piston areas times the piston stroke. The small piston can be fixed in position relative to

the lower cylinder resulting in dispensing volumes related to the larger piston area times the piston stroke.

A precision pump drive can contain at least one stepper motor or DC motor to control the linear motion of the pump piston, and usually another stepper motor or DC motor to control the rotation of the piston, with the exception for the special recovery and cleaning cases described earlier. This allows one of the pump's inlet or outlet ports to be aligned with the piston groove. The linear motion of the piston is generally created by the first stepper motor turning a ball screw. The ball screw nut, if held from rotating will move in a linearly fashion creating the necessary linear motion for the piston. A linear displacement sensor can monitor the position of the piston very accurately, and the entire system can be driven by a closed loop by a micro- controller. The preferred linear sensor for this application is a Renishaw 0.5 micron optical scale or similar scale, including magnetic linear scales and linear voltage differential transformers (LVDT' s) . The preferred stepper motors are 5 phase Oriental Nanostepper for the linear motion and 5 phase half step motors for the rotary motion. The Nanostepper motor, as supplied, has (16) discrete resolution ranges from 500 steps per revolution ^x to 125000. These ranges are operator selectable. The use of a nanostepper allows the drive to have an adequate number of steps between the 0.5 -micron Renishaw lines. For a THK 4 mm pitch ball screw it would require over 15 steps for the advance of the 0.5 pitch. The resolution can be selectable between inlet and outlet functions . It should be noted that other suitable stepper or DC motors can be used.

As an example, the pump can aspirate fluid into an inlet port at 10,000 steps per revolution and then dispense through an outlet port at 125,000 steps per revolution. Because of the stopped motion stability, simplicity to control and maintain accuracy, the preferred system contains stepping motors. It is also within the scope of the present invention for the linear drive to be a linear motor such as the stepper or dc Baldor Electric Company motor or nanomotion motor from Nanomotion, Ltd.

The pump system can be run orientated in various positions including horizontal and vertical as long as the position allows for air free dispensing. A microcontroller or digital signal processor is preferred to control the rotary and linear positioning. By entering information into the controller as to the desired amount of fluid to dispense, very precise dispensing can be accomplished because the entire resolution of the system is derived from the linear encoder. The movement of the piston can be controlled by several motion velocity profiles including the use of a Gaussian profile for smoothness of motion. To effectively dispense very small volumes, the controller can optionally interface with active nozzles. This interface, when used, can provide for synchronization of the piston functions with that of the active nozzle. The addition of optional analog to digital conversion (A/D) capability lets the system interface with external sources, such as a pressure transducer or other source .

DESCRIPTION OF THE DRAWINGS

Figure 1 shows a multiple diameter multiple port

two-piece pump.

Figure 2 shows a cross section of a multiple diameter multiple port two-piece pump.

Figure 3 shows an embodiment of a precision pump drive frame and electrical components .

Figure 4 shows slide and optical encoder components.

Figure 5 shows a possible controller system architecture.

Figure 6 shows an interface between an active nozzle and a controller.

Figure 7 shows a supervisory control sequence.

Figure 8 shows a single pulse dispensing cycle.

Figure 9 is a flowchart of a dispensing cycle.

Figure 10 shows a Gaussian motion algorithm.

Figure 11 shows a dual piston/cylinder pump cross section and port locations on upper piston.

Figure 12 shows a dual piston/cylinder pump cross section and ports located on upper and lower pistons.

Figure 13 shows a dual piston/cylinder pump cross section

with piston/cylinder motion.

DETAILED DESCRIPTION

Figure 1 shows a two diameter multiple port two-piece pump. It consists of a piston 1 and a cylinder 2. The piston is connected to a drive system using a keyed connector and a piston key, shown as 7. The lower connector 6, can also be keyed and fixed to the base of the drive assembly. A controller and position sensing sensors determine the piston rotary and linear positioning, relative to the fixed cylinder.

The piston outside diameter, and the cylinder internal diameter, have a very small clearance creating a fluid boundary layer seal. At a certain position along the cylinder are located inlet ports 3 and outlet ports 4. There are various tube fittings 5 available that simply screw into the inlet and outlet fitting rings.

Figure 2 shows how the fittings 10 are used to seal to the cylinder inlet/outlet ports. The inlet outlet ports 11 are shown as rectangular slots on the internal diameter of the cylinder and circular on the outside diameter where the fittings create seals. The port slots can also be circular holes . The piston can contain a groove on the larger diameter 8 and on the smaller diameter 9. Between the two diameters, an undercut can assist in pump manufacturing and act as the means to connect 8 and 9. In Figure 2, the groove is shown aligned on the two diameters, but the groove orientation can be rotated to each other as long as the undercut provides a continuous fluid path between 6 and 9. The grooves may also be different sizes.

Figures 3 and 4 show the pump and drive system overall components. The pump piston 12 and the cylinder can be coupled to the drive with keyed connectors 13. There are numerous connection devices that could be used here and are within the scope of the invention. The connectors could be linked to universal joints 14 to keep the piston and cylinder aligned and free from any bending loads during use. The bottom universal joint can be connected to the base frame, while the upper, or piston universal joint can be connected to a rod held in place by two angular contact bearings 15. These preloaded bearings can provide for piston rotation, but not for linear motion. A pulley can be mounted at the top end of the bearing shaft. The pulley, its associated belt 32 and a motor pulley 31 can provide a means for coupling the rotary stepper motor 30 to the piston.

The pulley can have inlet and outlet alignment notches so that an optical switch can sense rotary position. On a lower pulley flange is usually at least one notch that represents a home position for the rotary drive. The movable upper support 29 can provide for the rotary bearing mounting, rotary drive components and a mounting surface for the linear ball screw nut 28. A movable upper support 29 can be coupled to the linear ball guide 35. The figures show the upper support shifted relative to the ball guide 35 so that the piston can be seen outside of the cylinder. Normally these two surfaces are aligned, and the upper support fastened to the ball slide carriage using mechanical fasteners . Shown attached to the carriage are upper and lower limit magnetic switches, a home magnetic switch and an optical scale. The Renishaw optical head 34 can be fixed to the frame where it can sense the

position of the ball guide carriage. A ball guide rail 33 is shown attached to the base frame. An upper support 29 can be moved up and down by sliding on a linear guide rail assembly 33,35 as a result of the linear ball screw 27 rotations. A ball screw nut 28, attached to the upper support 29, provides the conversion of ball screw rotary motion to linear movement up or down. Force support, and elimination of axial motion, can be provided by a second set of angular contact bearings 26. The ball screw can be coupled to a stepper motor 24 with a shaft coupling 25.

Figure 3 shows a possible position where the controller 18 can mount to the frame 17. A plate 23 is where rotary driver 22, nanostepper drive 21, and five and twenty four volt (or any other voltage) power supplies 19, 20 can be mounted.

Figures 5-12 show details of a particular embodiment of a microcontroller system. It should be remembered that many other embodiments are within the scope of the present invention. This preferred embodiment is illustrated and described to teach the techniques and methods used in the invention.

A controller executes control sequences by using ultra high precision closed loop control of the linear position of the piston relative to the cylinder. The piston has two types of motion relative to the cylinder: linear and rotational. The linear motion can be generated by commanding a nanostepper motor or other accurate motor with real time feedback from an ultra high precision position sensor. A preferred linear sensor is a Renishaw optical scale with a resolution of 0.5

micrometer. Commanding a second stepper motor with feedback from two binary sensors generates, or open loop, causes the rotational motion of the piston relative to the cylinder. The control system can monitor the binary sensors to confirm the engagement of the specific input and output ports. Precision alignment of the slot on the piston with the appropriate port on the cylinder is critical for efficient operation of the pump. Therefore, the rotational control must be accurate enough to achieve correct alignment.

The preferred controller uses an Intel 80C196 microcontroller. Figure 5 shows the block diagram of the architecture of the chip-based controller system. This system can contain a 16 bit microcontroller (or other sufficient bus width) with a 10 bit or more A/D converter. A PSD4135G2 flash memory or other memory can be used to store the program and data. A RAM memory can optionally be battery backed. A JTAG port can be used to load and modify the program.

The preferred system has two or more motor control outputs. One is to a nanostep driver 50RFK for linear motion and the other is to a SD5114 driver for rotary motion of the piston relative to the cylinder. To control multi-port nozzle, the controller has an 8 digital output (expandable to 12 port) . There can be four analog input channels, one of which can optionally be used to monitor the pressure of the fluid.

The microcontroller also has an RS232 and CAN bus interface. Through the RS232 serial interface, a user can control the pump with a personal computer (PC) . Another

communication interface can be a CAN bus with which several pumps can be controlled via a network. Other functions of the system include Reset, emergency stop, manual dispense triggering, etc. For future applications, the system also has 4 channel digital input and 8 channel digital output which can be used to expand nozzle control, LED display, etc.

To use present invention for precision low-volume array dispensing, use of active nozzle is required. Since the volume can be less than microliter, dispensing through traditional tubes connected to the output port of unit is difficult at best. With such small volumes, the gravitational forces become negligible while the surface tension becomes dominant. A unit with an integrated active nozzle is as shown in Figure 6. The active nozzle acts as a secondary actuator to squeeze the fluid out of the output tube. The microarray interface provided on the controller can interface with the active nozzle driver. A command to move the piston can be synchronized to activate the nozzle resulting in micro drops.

Figure 7 shows a possible supervisory control algorithm. When the unit is switched on, the user has the option of choosing one of nine functions. With such a system architecture, new functions can easily be added without changing the hardware .

Example functions for the embodiments shown in Figs. 1-10 will now be described:

Fill Cycle: When this function is evoked, the piston first

rotates to a predefined port followed by the linear motion of the piston to its home position (bottom most position of the piston relative to the cylinder) . The piston is now rotated to align the with the input port, begin moving the piston upward to a pre-selected distance or to its full stroke, and stops when the pump is completely filled with the pre-selected volume of fluid. Figure 8 shows the flow chart of a fill cycle .

Pump Cycle : This function normally begins after the fill cycle. When chosen, the piston rotates to align its slot with the appropriate output port if it is not already in that position, and then moves downward until it reaches its home position thereby dispensing the full capacity of the pump; it then stops .

Dispense Cycle: This function is different from the pump cycle. In this cycle, the user has the option to select any quantity of fluid that must be dispensed as long as it is less than its maximum capacity. The controller begins by rotating the piston to align its slot to the appropriate output port if it is not already there. The piston is then commanded to move downward in one of two modes: single Pulse or multiple pulse.

In single pulse, the piston moves down by one motor step dispensing the smallest volume possible with the system. In multiple pulses, the nanostep motor is commanded to move by a preselected number of pulses. The dispense cycle is shown in Figure 9.

Prime Cycle: In this function the pump is commanded to home position followed by fill cycle and pump cycle in succession.

The prime cycle can be either single or multiple depending upon the fluid properties that is being handled.

Load and Unload Pump: The user can invoke this function to change the pump. This requires first unloading the existing pump and then loading the new pump followed by a pump size algorithm. The unloading command usually initiates the piston to rotate to a predefined port, move to go to its home position, rotate the piston, and display a signal indicating it has reached its unloading position. Similarly, the loading the pump algorithm moves the pump to its loading position.

Calibration Cycle: The calibration cycle gives the feature of updating the calibration of the pump. This is usually required every time the pump is changed. The cycle begins with home position, fill cycle, and dispense cycle. The output from the port will be weighed or sized by optical means to update the calibration table.

Pump Size: This function is used when a new pump has to be installed on the units. A database of all available pumps will be available from which the user selects the pump of his/her choice. The program then calculates all the relationships between the stroke length and the volume and makes that as its current database.

Home : The home position is achieved by both the rotary and linear obtaining home signals. The home of the rotary motion can be found using the two binary sensors. These are optical sensors that detect when the piston rotates so that its slot aligns with the input port. The optional slots in the pulley can act as the means to align the slot of the piston to the

desired port. The linear motor home is achieved by monitoring a linear scale pulse that can be generated when the piston moves relative its bottom most position. The optical sensor output signal includes home pulse output.

Verify pump loaded: This function confirms the proper loading of the pump . A binary switch at the interface between the piston and the universal joint can be used to sense the presence of the pump. The controller forbids any motion of the piston until this becomes true.

Most of the controller's functions have a task of moving the piston relative to the spindle along their axis. The accuracy of this motion dictates the overall accuracy of the pump. One unique feature of this low-cost ultra high precision pump is that these linear motions are made precise by using a real time closed loop control of the piston relative to the cylinder. Furthermore, a Gaussian speed profile can be used to eliminate unwanted impact motion and avoid missed steps.

When moving the piston for filling, dispensing, priming, etc., it is desirable to have a speed profile so that jerks can be avoided during starting and stopping. Sudden motions of the piston relative the cylinder, in addition to creating undesirable jerks, have a tendency to increase the work load on error compensation Therefore to achieve a smooth motion, a Gaussian speed profile is chosen.

The linear motion of the piston relative to the cylinder used in all the functions discussed so far is

achieved by using a Gaussian profile for speed. Figure 10 shows the flowchart of the typical Gaussian algorithm used for the linear motion. Once the distance to be moved is input by the user, a Gaussian speed table is generated. A speed versus distance profile is created for the required distance to be moved. The speed of the nanostepper motor can be changed by changing the time delay, hence the pulse width. The time delay can be calculated by finding the inverse of the calculated speed and be tabulated for the respective step. Then the single or multiple dispense cycle can be called with the Gaussian profile incorporated. This is shown in Figure 10.

One unique feature of the present invention is the integration of a real-time closed loop position control of the linear motion of the piston relative to the cylinder. In operation, once the user selects the distance the piston must move, the controller first generates a speed table to fit a Gaussian profile as explained before. Following this table, the controller commands the nanostepper motor to raise or lower the piston and start monitoring the position of the piston. The position of the piston relative to the cylinder can be obtained by measuring the relative motion between the rail and carriage. The position sensor, an optical sensor in this embodiment, outputs digital quadrature signals that are fed to two high speed digital input (HSI) channels of the controller. The total number of transitions on two quadrature channels is proportional to the distance traversed by the piston relative to the cylinder.

There are at least two possible control algorithms, multiple pulse and single pulse, which are used in each of the

linear motion. First, a multiple pulse motion can be initiated using a multiple pulse motion algorithm. In this algorithm, the nanostepper is commanded through high-speed output (HSO) channel to go up to a predetermined distance (a large percentage f the stroke in this embodiment) following the Gaussian table for speed control. At the same time, the quadrature pulses output from the sensor are counted to keep track of the actual position moved.

Once the multiple pulse motion is complete, the controller can initiate the single pulse algorithm. First the error in position, if any, is calculated. Then the actual position can be calculated using the counter values stored and compared with the expected position of the piston relative the cylinder. If the motor missed any pulse commands due to overload, overspeed, or for any other reason, the error will be non-zero. Once the error is known, the controller will start sending out single pulse commands to the nanostepper and verify the motion for each pulse. In other words, the motion can be controlled by checking the motion associated with each step in real-time. This method can slow down the speed, but this is not too important because it occurs in the Gaussian region where the speed is very low in preparation to stopping the motion. Furthermore this region is very small (a small percentage of the stroke in this embodiment) compared to the total motion of the piston.

This two stage algorithm enabled optimum balance between the need for ultra high precision real time control and overall dispensing speed.

The rotary position can be determined using two binary- optical sensors and two circular disks with slots. The top and bottom side of the rotary pulley can serve as the two circular disks. The top portion of the pulley can have a single slot cut, while the bottom portion of the pulley can have ten slots (or other number) corresponding to ten ports in the cylinder, or vice versa. The number of slots depends on the number of input and output ports of the pump. The slots are cut in such a way that the bottom ten slots are spaced equally, and one of the slots matches with the top slot. In this embodiment, there are two optical sensors used to sense these slots. They are positioned in such a way that the top rotary sensor sees the slot in the top portion of the pulley while the bottom sensor sees the ten slots in the bottom portion of the pulley. The home and port positions can be also reversed.

When both the sensor outputs are reading a high (or low depending on the circuit configuration) , both top and bottom slots are aligned to form the home position. At all other times, the top sensor gives a low output while the bottom sensor alternates between low and high depending on whether the ports are in position or not .

To use invention in yet another scenario of custom dispensing fluid into a container, a hand held dispensing device is usually required. This device can be equipped with a trigger mechanism that will initiate the motion of the piston in units. The user selects the volume to be dispensed in advance, then positions the device at the desired location and presses the trigger that initiates the pumping action on

the unit .

The present invention can use different velocity and acceleration profiles (i.e. ramp-slew curves) including a Gaussian profile. The Gaussian profile can use 1000 stepper pulses per revolution to get the piston close to the linear optical encoder position of choice. When the piston is within a few steps of the true position, the motor resolution can be switched to 10,000 pulses per revolution. In this region, the motor can single step to the correct final position. At the switch point, the microprocessor can review the number of steps and encoder lines to determine if there is any error, i.e. outside a user selectable error window. This quality control feature can be used on every aspiration and dispensing cycle .

Optionally, an onboard A/D converter can provide additional criteria against which the move can be compared. For example, an external pH meter can be fed into the A/D. During the dispense cycle, a pH can be read, and the pump can be stopped early when that predetermined pH is reached. For example, a compound command such as "pump 100 ml, but do not exceed pH 4" could be issued. The pump will attempt to pump the 100 ml of fluid, but if the threshold pH is reached first, the dispense cycle will be stopped early.

Figs. 11-13 show a different embodiment of pump. Here a double piston pump can be used. There is an upper piston and cylinder of a first diameter and a lower piston and cylinder of a second diameter. The pistons can be linked together and moved by an external coupling bar. The upper piston can move

up and down as well as rotate; the lower piston usually only can move up and down (however can optionally rotate) . The top and bottom pistons do not need to touch; rather, a fluid boundary between the two pistons acts as a coupling and a pivot (when the top piston rotates) . If the two pistons were physically coupled, there would need to be a rotary bearing between them (or that they both rotate together) . This bearing becomes unnecessary with a fluid boundary. Also, any physical coupling causes piston wear. This is avoided with the fluid boundary.

Fig. 11 shows an embodiment of a dual piston/cylinder multiple port pump. It consists of a piston 35 and a cylinder 47 making up the upper section of the pump. The lower section of the pump consists of piston 40 and cylinder 44. The upper piston can be connected to a drive system using a keyed connector and a piston key, shown as 36. A lower connector 43, can also be keyed in two places with the first being fixed to the base of the drive assembly at a position 42. The lower piston can also be fixed to the lower cylinder at position 41. The upper piston/cylinder and lower piston/cylinder can be coupled together using a Tri Clover connection or may be screwed together at a point 39. The upper piston/cylinder can contain inlet and outlet ports 46, 37. The inlet and outlet ports can use HPLC or other fittings 38 to connect tubing to the dual piston/cylinder pump. A controller and position sensing sensors can determine the piston rotary and linear positioning relative to the fixed cylinder. The piston outside diameter, and the cylinder internal diameter, normally have a very small clearance thus creating a fluid boundary layer seal. At a certain position

along the cylinder inlet ports 46 and outlet ports 37 can be located. Different fittings 38 can screw into the inlet and outlet fitting ring, 45.

Fig. 12 shows a second embodiment of a dual piston/cylinder pump where the lower piston 52 contains a piston groove and can rotate to several inlet or outlet ports on the lower piston/cylinder. This configuration can have inlet 48 and outlet 54 ports on the upper pump section 49. It can also have other inlet ports or outlet ports 17. The lower piston normally has a piston groove 51 which acts as a valve mechanism. The upper piston can have a similar groove 53. The inlet/outlet ports 48,50 can be rectangular slots on the internal diameter of the cylinder and circular on the outside diameter where the fittings create seals. The port slots can also be circular holes. In Fig. 12, the grooves are shown aligned on the two diameters, but the groove orientation can be rotated as long as the two cylinders provide a continuous fluid path between the groove 51 along the interface between the piston 21 and the piston 52. The grooves may also be different sizes.

Fig. 13 shows how pump motion can be controlled by an external drive system. A dual piston/cylinder pump 60 can be coupled to a drive with keyed connectors 57 and 58. There are numerous connection devices that could be used and are within the scope of the present invention. The connectors could be linked to universal joints 59 to keep the pistons and cylinders aligned and free from any bending loads during use.

The bottom universal joint can be connected through a link mechanism 61 to the top piston. The upper piston universal

joint 56 also can rotate so that a groove can index to various inlet or outlet ports. The upper and lower pistons are normally coupled so that vertical motion causes both the upper and lower pistons to move together. Rotary motion can be accomplished independently of any vertical motion. The piston link mechanism can also be held fixed while the pump assembly 60 is vertically moved.

Example functions for the embodiments shown in Figs. 11- 13 will now be described:

Pump Cycles (Fig. 11 pump) : The upper piston first rotates to a predefined port followed by the linear motion of the upper and bottom pistons linked together to its home position (bottom most software position of the piston relative to the cylinder) . The upper piston is now rotated to align with an input port. The drive begins moving the pistons upward to a pre-selected distance or to the full stroke, and stops when the pump is completely filled with the pre-selected volume of fluid. This volume defined by the difference in piston areas times the vertical motion or stroke. In an alternate configuration, the lower piston is pinned fixed to the piston cylinder and only the top piston moves vertically and indexes. The fill volume is defined as the upper piston area times the vertical motion or stroke.

Pump Cycles (Fig. 2 pump) : The lower piston aligns with the appropriate inlet port while the upper piston aligns to a position where there are no ports. This is followed by the linear motion of the upper and bottom pistons linked together to its home position (bottom most software position of the piston relative to the cylinder) . The lower piston is now

rotated to align with an input port, the upper piston being in a blocked port position. The pistons begin moving upward to a pre-selected distance or to the full stroke, and stop when the pump is completely filled with the pre-selected volume of fluid. This volume defined by the difference in piston areas times the vertical motion or stroke. The upper piston then moves to the selected outlet port while the lower piston indexes to a blocked port position. The piston set then moves vertically until the home position is reached corresponding to a full dispense of fluid. At the home position, the system can index to either an inlet or outlet position on the lower cylinder followed by an index of the upper piston to an inlet whereby air could be introduced forcing all the pump internal fluid out of the pump. This minimizes any waste of valuable product as it can be reclaimed using the proper port selection.

It should be remembered that the pistons can be moved with the cylinders being held fixed or the pistons can be held fixed for vertical motion and the cylinders moved relative to the fixed pistons.

The present invention has been explained by various descriptions and illustrations. A person skilled in the art will understand that there are many changes and variations that are within the scope of the present invention.