AND 7-PROTECTED-10-DEACETYLBACCATΪN πi

DERIVATIVES FROM 10-DEACETYL TAXOL A,

10-DEACETYL TAXOL B, AND 10-DEACETYL TAXOL C Description

Technical Field

The present invention relates to a process for converting purified, partially purified or crude taxane mixtures into a protected precursor of 10-deacetylbaccatin m or into 10-deacetylbaccatin HI. Specifically, the present invention relates to a three-step process whereby in the first step the 2'-OH and the 7-OH groups of 10-deacetyl taxol A, B or C are protected using a suitable protecting group. Cleavage of the side chain located at the C- 13 position occurs during the second step of the process resulting in a protected precursor of 10-deacetylbaccatin HI. Deprotection occurs during the third step resulting in 10-deacetylbaccatin HI. Background Art Between the years 1958 and 1980, extracts of over 35,000 plant species were tested for anticancer activity as part of an NCI-sponsored program. Chemists Monroe E. Wall and M.C. Wani first isolated a crude extract concentrate from yew tree (Taxus brevifolia) bark and wood samples in 1963. Initial screening showed the extract to be a potential anticancer agent, being very active against an unusually wide range of rodent cancers. Isolation of the active agent in the crude extract took several years due to the very low concentrations of the agent present in the plants. The active agent was identified, the structure determined and the compound was named taxol (I), in 1971.

H

Despite taxol's excellent activity in model tumor systems, clinical trails were delayed owing to short supplies of the drug and formulation problems related to the drug's low water solubility. However, great interest in the drug was rekindled when it was discovered in 1979 by Susan B. Horwitz and co- workers that a unique mechanism for taxol's antitumor activity involved cell microtubules. See, Nature 277:665-667 (1979). Microtubules play a key role in mitosis, maintenance of cell shape, cell motility, and intracellular transport. They are self-assembling and self-disassembling structures that are in dynamic equilibrium with tubulin dimers, the protein subunits of which they are composed. A substance that interferes with microtubules can disrupt cell growth and function.

The 1979 study by S. Horwitz et al., reported that the binding of taxol to tubulin acts to stabilize cell microtubules and to prevent their depolymerization. Thus, taxol increases the time required for cell division which in turn inhibits tumor activity. Discovery of this unique mechanism, by which taxol disrupts the proliferation of cancerous cells, intensified research interest in the drug, and the National Cancer Institute (NCI) began a concerted effort to obtain taxol for clinical trials. In ongoing clinical trials, taxol has shown promising results in fighting advanced cases of ovarian, breast, and other cancers. Recently, taxol was approved by the Food and Drug Administration for the treatment of refractory ovarian cancer; however, taxol is extracted in limited quantities from a natural vegetation that is in short supply.

There have been some methods developed for increasing the supply of taxol. For example, tissues of T xus brevifόlia have been successfully cultured to produce taxol, related alkaloids, and alkaloid precursors, as disclosed in U.S. Patent No. 5,019,504 issued to Christen et al. Turning toward an alternative route, Holton et al.,JACS 110:6558 (1988) proposed a synthetic route, directed to the synthesis of the tetracyclic taxane nucleus from commodity chemicals. Despite the progress made in this approach, the final total synthesis of taxol is, nevertheless, likely to be a multi-step, tedious, and costly process. An alternate approach to the total synthesis of taxol has been a partial synthesis or semi- synthetic route, involving the use of related alkaloid precursors, collectively referred to as taxanes. U.S. Patent No. 4,924,011 issued to Denis et al., discloses a process whereby taxol is prepared from a derivative of 10-deacetylbaccatin m or baccatin m. The U.S. Patent No. 4,857,653 issued to Colin et al. discloses processes for the preparation of taxol and 10-deacetyl taxol from baccatin in and 10- deacetylbaccatin m, respectively. Carver et al., in his U.S. Patent No. 5,202,448, discloses a method whereby partially purified mixtures of naturally occurring taxanes containing taxol and cephalomannine are converted into baccatin HI.

The above U.S. patents and technical paper by Holton each disclose a process whereby the supply of taxol may be increased; however, the amount of taxol actually produced by way of tissue

cultures as disclosed by Christen et al. is minute, and although 10-deacetyl taxol is known to promote in vitro the polymerization of tubulin and to inhibit, at the same time, the depolymerization of microtubules, the only taxane currendy approved for use as a chemotherapeutic agent is taxol. Consequently, other naturally occurring taxanes that are similar in structure such as 10-deacetyl taxol are useful and valuable only if they can be ultimately converted to taxol. The semi-synthetic route disclosed in the Denis et al. patent is unfortunately limited by d e precursors required for the starting material. The Carver et al. patent addresses this need by disclosing a process whereby baccatin m, a precursor for the semi-synthetic route may be obtained. However, the use of sodium borohydride as a reducing salt resulted in an undesired epimerization at the C-7 position, decreasing me yield of baccatin JR. To counteract the epimerization event at the C-7 position, Carver et at. discloses the use of Lewis acids. The heavy metal halides used are extremely toxic and it would be undesirable to introduce this type of toxic into a process for a pharmaceutical drug.

There is still a need, therefore, for a process to convert other naturally occurring taxanes to taxol or to taxol precursors that can be easily converted into taxol. Disclosure of Invention

Accordingly, it is an object of this invention to provide a method to convert partially purified or crude taxane mixtures into 10-deacetylbaccatin m.

Another object of the present invention is to provide a method to convert partially purified or crude taxane mixtures into 7-protected-lO-deacetylbaccatin JR. Another object of the present invention is to provide a method to convert selected pure taxanes into 10-deacetylbaccatin m.

A further object of the present invention is to provide a memod to convert selected pure taxanes into 7-protected-lO-deacetylbaccatin JR.

Additional objects, advantages and novel features of this invention shall be set forth in part in the description and examples that follow, and in part will become apparent to those skilled in the art upon examination of the following specification or may be learned by the practice of the invention. The objects and advantages of the invention may be realized and attained by means of the instrumentalities, combinations, compositions, and methods particularly pointed out in the appended claims. To achieve the foregoing and other objects and in accordance with the purposes of the present invention, as embodied and broadly described therein the method of mis invention comprises contacting a mixture containing at least one taxane compound having an ester linkage at the C-13 position with a hydroxy protecting group and then cleaving the ester linkage resulting in a taxol intermediate having the hydroxyl group positioned at C-7 protected, the deprotection of which

furthermore leads to a known taxol intermediate. Best Mode for Carrying out the Invention

The present invention relates to a high yield process for converting purified, partially purified or crude mixtures of naturally occurring taxanes containing 10-deacetyl taxol A, 10-deacetyl taxol B, or 10-deacetyl taxol C into 7-protected-lO-deacetylbaccatin in and 10-deacetylbaccatin JR. The structure of 10-deacetyl taxol A (R)

has the basic structure of taxol with R _t =Ph. The structures of 10-deacetyl taxol B and C are similar to formula (R) above except that R^ /= and n-CjH _u , respectively. The structure of 10-deacetylbaccatin HI (HI)

has the basic diterpenoid structure of taxol without the side chain at the C-13 position. The structure of 7-Protected-lO-deacetylbaccatin m is similar to the general formula (HI) above except the hydroxyl group at the C-7 position is protected.

The preferred embodiment of the present invention requires three steps. The first step includes contacting a solution containing the general formula (R) with a protecting group, thus leading to the protection of the hydroxyl groups located at the C-7 and C-2' positions. The second step includes contacting the resulting 2',7-protected-lO-deacetyl taxol A, B or C, with an ester cleavage agent to effectively cleave the ester linkage at the C-13 position resulting in the general formula (RJ) having the hydroxyl group at the C-7 position being protected. The third and final step includes

reacting the product obtained from the preceding step widi a deprotecting agent resulting in the deprotection of die hydroxyl group located at die C-7 position which gives rise to the final product of 10-deacetylbaccatin m.

This invention includes a process for the preparation of 10-deacetylbaccatin m and 7- protected-10-deacetylbaccatin in derivatives from purified, partially purified or crude mixtures of 10- deacetyl taxol A, 10-deacetyl taxol B and 10-deacetyl taxol C. The following description will only make reference to 10-deacetyl taxol A; however, it is to be understood, as supported by die Examples which follow, that with each and every occurrence of die term 10-deacetyl taxol A it is contemplated mat 10-deacetyl taxol B, 10-deacetyl taxol C or any mixtures thereof may be substituted. The first step in the process, protection of the C-7 and C-2' positioned hydroxyl groups of 10-deacetyl taxol A, is preferably accomplished by contacting, at 0-25 °C overnight, a purified solution of 10-deacetyl taxol A with a protecting group, such as triethylsilyl chloride. In one example of the process the protecting group triethylsilyl chloride (E^SiCl) is employed due to its stability, cost, and ease in use and removal. Furthermore, protection of the hydroxyl group at the C-7 position eliminates concern of epimerization as discussed in the background section above. However, a variety of protecting groups for the hydroxyl groups and syndiesis thereof may be found in "Protective Groups in Organic Synthesis" by T.W. Green, John Wiley and Sons, 1991.

The preferred next step in the process is the basic hydrolysis or reduction of the ester linkage at the C-13 position, resulting in the general formula (HI), with the hydroxyl group at the C-7 position being protected. Reduction of an ester in base is an extremely efficient and irreversible reaction. As such, reduction often gives better yields of carboxylic acids and alcohol than does acid hydrolysis. In this preferred second step 2',7-protected-lO-deacetyl taxol A is reacted with sodium borohydride, at room temperature, for twenty-four hours resulting in the cleavage of the ester linkage at the C-13 position producing 7-protected-10-deacetylbaccatin HI, having general formula (HI), or 7-Et,Si-10-deacetylbaccatin HI. The conversion does not result in a significant loss of any of the 7- Et,Si-10-deacetylbaccatin JR. Furthermore, the 7-Et ₃ Si-10-deacetylbaccatin in has potential commercial value as an intermediate in the synthesis of taxol. The specific protecting group used only has to be compatible with the chemical conversion used in mis process.

The preferred final step in the process of the present invention, deprotection of e C-7 positioned hydroxyl group of 7-Et ₃ Si-10-deacetylbaccatin HI, is accomplished by reacting a weak acid, such as 1% HCl/MeOH, at 0°C for three hours widi the compound 7-EtjSi-lO-deacetylbaccatin m to produce 10-deacetylbaccatin m.

In the second embodiment of e present invention the hydrolysis step described previously is accomplished by contacting 2',7-protected-lO-deacetyl taxol A with an aqueous solution of NaOH,

at room temperature, for twenty-four hours.

In the third embodiment of me present invention the hydrolysis step described in die preferred embodiment is accomplished by contacting 2',7-protected-10-deacetyl taxol A with a solution of NH ₃ /MeOH, at room temperature, for twenty-four hours. In the fourth embodiment of me present invention the hydrolysis step described in the preferred embodiment is accomplished by contacting 2',7-protected-10-deacetyl taxol A with a Lewis acid, such as, cesium fluoride or lithium iodide, at room temperature overnight.

In the fifth embodiment of the present invention a crude mixture of 10-deacetyl taxol A is converted into both 7-protected-lO-deacetylbaccatin in and 10-deacetylbaccatin HI as described in the prior three embodiments.

The following non-limited examples provide specific high yield processes for preparing 7- protected-10-deacetylbaccatin in and 10-deacetylbaccatin from purified, partially purified or crude mixtures of taxanes containing 10-deacetyl taxol A, 10-deacetyl taxol B or 10-deacetyl taxol C. All scientific and technical terms have the meanings as understood by one wi i ordinary skill in the art. Η and ¹³ C NMR spectra were recorded on a Varian Gemini-400 instrument. The chemical shifts are expressed in ppm relative to the reference of CDC1 ₃ or DMSO. Deuterated solvents were purchased from Aldrich Chemical Co. IR was measured on a KVB Analect Diamond-20 FT-JR Spectrometer featuring a Laser Precision XAD-Plus Microscope. Electrospray mass spectra were obtained from a VG Platform HPLC-MASS Spectrometer. TLC plates of silica gel 60F254 were purchased from E.M. Merck and kept in a closed container over Drierite* prior to use. Melting points were measured on a MEL-TEMP R apparatus equipped widi a digital Burnant 100 Thermocouple Thermometer and are uncorrected. HPLC was performed on a Hitachi chromatographic spectrometer (L-6200A Intelligent Pump, D-600 Interface, L-4000 UV Detector and AS-4000 Intelligent Auto Sampler). Combination of CH ₃ CN and H ₂ O in different concentrations are used as HPLC solvent system. All solvents were distilled before use. Commercially available chemicals were used without any further purification. Various methods of purifying the products of die present invention are known and understood by those skilled in the art and d e purification methods presented in die Examples is solely listed by way of example and is not intended to limit the invention.

EXAMPLE I A. Preparation of 2',7-Bis(Et ₃ Si)-10-DAT: 62 mg (0.08 mmol) of 10-deacetyl taxol A and 2 ml of pyridine were introduced under an argon atmosphere into a 25 ml round bottomed flask equipped widi a magnetic stir bar. 300 μ\ (20-eq) of triethylsilyl chloride was added via a syringe. The mixture was then stirred at room temperature for twenty-four hours.

A few drops of water were then added to the mixture. The reaction mixture presumed to

contain 2',7-Bis(EtjSi)-10-DAT was extracted twice using methylene chloride and water (50:50 by volume). The organic phase was collected, dried over anhydrous magnesium sulfate and subsequendy filtered. The solvent was removed under reduced pressure to obtain a colorless oil as crude product. The crude product was purified by flash column chromatography on silica gel and eluting with 25% ethylacetate hexane. The selected fractions were evaporated to dryness widi a rotary evaporator under reduced pressure. 79 mg of 2',7-Bis(Et ₃ Si)-10-DAT were recovered resulting in a 99% yield. TLC: silica gel, Rf=0.23 for 2', 7-Bis(Et ₃ Si)-10-DAT at 7% methanol/mediylene chloride solvent system. The compound is identical to the known compound. B. Side Chain Cleavage to form 7-Et ₃ Si-10-DAB: 0.138 ml of an (1.34M,l-eq) ammonium/methanol solution and 0.8 ml of methanol was added to a 4 ml vial, equipped with a magnetic stir bar, having 11.3 mg (0.011 mmol) of 2',7-Bis(Et ₃ Si)-10-DAT. The reaction was stirred at room temperature for twenty hours and die solvent was removed under reduced pressure. The residue was redissolved in methylene chloride and die resulting organic solution was then extracted twice with 20 ml methylene chloride/water (50:50 by volume). The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to obtain 7-EtjSi-10-DAB as a major product and die side chain as a by product. The proton nuclear magnetic resonance spectrum (400 MHz; deuterated chloroform; chemical shift in ppm; coupling constants J in Hz): Η NMR (400 MHz, CDC1 ₃ ) δ 8.0855 (d, J=7.68 Hz, 2H), 7.5773 (t, J=7.2 Hz, IH), 7.4485 (t, J=7.2 Hz, 2H), 5.5793 (d, J=6.9 Hz, IH), 5.1459 (d, J= 1.52 Hz, IH), 4.9415 (dd, J=8.7, 0.5 Hz, IH), 4.8304 (bm, IH), 4.3791 (dd, J=10.4, 6.6 Hz, IH), 4.2973 (d, J=8.48 Hz, IH), 4.2478 (d, J= 1.56 Hz, IH), 4.1434 (d, J=8.44 Hz, IH), 3.9285 (d, J=7.0, Hz, IH), 2.4250 (d, IH), 2.2595 (s, 3H), 2.2425 (dd, IH), 2.2252 (dd, IH), 2.1200 (m, IH), 2.0528 (s, 3H), 1.8655 (ddd, IH), 1.7054 (s, 3H), 1.6415 (bs, IH), 1.24505 (bs, IH), 1.0483 (s, 3H), 1.0483 (s, 3H), 0.9082 (t, J=7.96, Hz, 9H), 0.5264 (m, 6H). MS (electrospray m/e calculated for C ₃₅ H ₅₁ O ₁₀ Si: 658, found 659.1(M+H) ⁺ . TLC: Rf=0.32 using 5% methanol/methylene chloride solvent system, chromatography purity: 90.4%. The proton NMR and melting point of the product matched the previously reported literature values for 7-Et ₃ Si-10-DAB, reported by A.E. Greene, "A Highly Efficient, Practiced Approach to Taxol", J. Am. Chem. Soc, 1988, 110:5917-5919, 1988. C. Deprotection of T-EtaSi-lO-DAB: 25.3 mg (0.038 mmol) of 7-Et ₃ Si-10-DAB, 1 ml methylene chloride and 1 ml methanol are introduced into a 25 ml round bottomed flask equipped with a magnetic stir bar which was placed at 0°C (ice bath). A 0.5 ml solution of 1% hydrochloric acid (39%) in methanol was then added to the mixture and stirred at 0°C for 3 hours.

The solution was then diluted with the addition of 10 ml methylene chloride and extracted twice with 20 ml of saturated sodium bicarbonate, water. The organic layer was then dried over anhydrous magnesium sulfate after. Following the removal of 15.4 mg of

10-DAB was obtained as a major product having a 72% yield. The proton NMR and melting point of the product matched the previously reported literature values for 10-DAB.

EXAMPLE H

A. Preparation of 2',7-Bis(Et ₃ Si)-10-DAT: Prepared in the same manner as described above in Example I.

B. Side Chain Cleavage to form 7-Et ₃ Si-10-DAB: 1 ml of methylene chloride/methanol (50:50 by volume) and 5 mg of sodium borohydride was added to a 4 ml vial, equipped with a magnetic stir bar, having 4.6 mg (0.005 mmol) of 2',7-Bis(Et ₃ Si)-10-DAT. The reaction was stirred at room temperature overnight and the solvent was removed under reduced pressure. The residue was redissolved in methylene chloride and the resulting organic solution was then extracted twice with 20 ml methylene chloride/water (50:50 by volume). The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to a obtain a crude product. The resulting product proved to be identical to diat obtained in Example I above, as analyzed by TLC and HPLC.

C. Deprotection of 7-EtjSi-lO-DAB: The procedure for deprotection set out in Example I above was followed.

EXAMPLE m

A. Preparation of 2',7-Bis(Et ₃ Si)-10-DAT: Prepared in the same manner as described above in Example I.

B. Side Chain Cleavage to form 7-Et,Si-10-DAB: 0.5 ml of methanol and 10 drops of a 1 % solution of sodium hydroxide was added to a 4 ml vial, equipped with a magnetic stir bar, having 5.0 mg (0.500 mmol) of 2',7-Bis(Et ₃ Si)-10-DAT. The reaction was stirred at room temperature for four hours and the solvent was removed under reduced pressure. The residue was redissolved in methylene chloride and the resulting organic solution was then extracted twice with 20 ml methylene chloride/water (50:50 by volume). The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to obtain a crude product. The resulting product proved to be identical to that obtained in Example I above, as analyzed by TLC and HPLC.

C. Deprotection of 7-EtjSi-10-DAB: The above procedure for deprotection set out in Example I above was followed.

EXAMPLE IV A. Preparation of T* 7-Bis(Et ₃ Si)-10-DAT'B: 24 mg (0.03 mmol) of Deacetyl taxol B and 0.5 ml of pyridine were introduced into a 25 ml round bottomed flask equipped with a magnetic stir bar. 60.0 μl (12eq) of triethylsilyl chloride was added under an argon atmosphere at 0°C. The mixture was then stirred at room temperature for twenty-four hours.

A few drops of water were then added to the mixture. The reaction mixture presumed to contain 2',7-Bis(Et ₃ Si)-10-DATB was extracted twice using methylene chloride and water (50:50 by volume). The organic phase was collected, dried over anhydrous magnesium sulfate and subsequently filtered. The solvent was removed under reduced pressure to obtain a colorless oil as crude product. The crude product was purified by flash column chromatography on silica gel and eluting with 25 % ethylacetate/hexane. The selected fractions were evaporated to dryness widi a rotary evaporator under reduced pressure. 24.2 mg of 2',7-Bis(Et ₃ Si)-10-DATB were recovered resulting in a 78.2% yield. TLC silica gel, Rf=0.24 for 2\7-Bis(Et ₃ Si)-10-DATB at 25% ethylacetate/hexane solvent system and Rf=0.67 for 2',7-Bis(Et ₃ Si)-10-DATB at 7% methanol/methylene chloride solvent system. The portion nuclear magnetic resonance spectrum (400 MHz; deuterated chloroform; chemical shift in ppm; coupling constants J in Hz); IH NMR (400 MHz, CDCL,) d, 8.11 (d, J=8.0 Hz, 2H), 7.57 (t, J=8.0 Hz,lH), 7.48 (t, J=8.0 Hz, 2H), 7.34 (t, J=8.0 Hz, 2H), 7.26 (d, J=8.0 Hz, 4H), 6.68 (d, J=8.0 Hz, IH), 6.42 (q, J=8.0 Hz, IH), 6.27 (t, J=8.0 Hz, IH), 5.65 (d, J=4.0 Hz, 4H), 5.53 (d, J=4.0 Hz, IH), 5.09 (d, J=1.8 Hz, IH), 4.92 (d, J=8.0 Hz, IH), 4.59 (d, J=2.16 Hz, IH), 4.38 (dd, J=12.0, 8.0 Hz, IH), 4.25 (d, J=2.0 Hz, IH), 4.20 (d, J=8.0 Hz, IH), 3.86 (d, J=4.0 Hz, IH), 2.49 (s, 3H), 2.42 (m, 2H), 2.31 (m, IH), 2.10 (m, IH), 1.91 (m, IH), 1.91 (s, 3H), 1.78 (s, 3H), 1.73 (s, 3H), 1.70 (d, J=8.0 Hz, 3H), 1.61 (s, IH), 1.55 (m, 2H), 1.23 (s, 6H), 1.09 (s, 3H), 0.91 (t, J=8.0 Hz, 9H), 0.78 (t, J=8.0 Hz, 9H), 0.77 (t, 3H), 0.53 (m, 6H), 0.40 (m, 6H); TLC: Rf=0.24, 25% EtOAc/hexane; Rf=0.67, 7% MeOH/CH ₂ Cl ₂ . HPLC: RT=20.52, C18, MeCN/H ₂ O, (40-100%). B. Side Chain Cleavage to Form 7-Et ₃ Si-10-DAB: 3.7 mg (lOeq) of sodium borohydride was added to a 4 ml vial, equipped wid a magnetic stir bar, having a solution of 10 mg (0.009 mmol) of 7-Bis(Et ₃ Si)-10-DAT in 0.5 ml methanol. The reaction was

stirred at room temperature for twenty hours and d e solvent was removed under reduced pressure. The residue was redissolved in methylene chloride and the resulting organic solution was then extracted twice with 20 ml methylene chloride/water (50:50 by volume). The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to obtain 7-Et ₃ Si-10-DAB as a major product and d e side chain as a by-product. The resulting product proved to be identical to d at obtained in Example I above, as analyzed by TLC and HPLC.

C. Deprotection of 7-Et ₃ Si-10-DAB: The procedure for deprotection set out in Example I above was followed. EXAMPLE V

A. Preparation of 2',7-Bis(EtjSi)-10-DATC: 164 mg (0.20 mmol) of 10-deacetyl taxol C and 4 ml of pyridine were introduced into a 25 ml round bottomed flask equipped with a magnetic stir bar. 0.684 ml (20-eq) of triethylsilyl chloride was added under an argon atmosphere at 0°C. The mixture was then stirred at room temperature overnight. A few drops of water were then added to d e mixture. The reaction mixture presumed to contain 2',7-Bis(Et ₃ Si)-10-DATC was extracted twice using methylene chloride and water (50:50 by volume). The organic phase was collected, dried over anhydrous magnesium sulfate, and subsequently filtered. The solvent was removed under reduced pressure to obtain a colorless oil as crude product. The crude product was purified by flash column chromatography on silica gel using air pressure and eluting with 20% ethylacetate/hexane. The selected fractions were evaporated to dryness with a rotary evaporator under reduced pressure. 155.6 mg of 2',7-Bis(Et ₃ Si)-10-DAT were recovered resulting in a 82% yield. Η NMR (400 MHz, CDC1 ₃ ) δ 8.11 (d, J=7.04 Hz, 2H), 7.57 (t, J=7.20 Hz, IH), 7.48 (t, J=7.24 Hz, 2H), 7.35 (t, J=7.72 Hz, 2H), 7.29 (d, J=7.00 Hz, IH), 7.25 (d, J=7.12 Hz, IH), 6.38 (d, J=9.12 Hz, IH), 6.27 (t, J=8.80 Hz, IH), 5.66 (d, J=7.20 Hz, IH), 5.53 (d, J=9.00 Hz, IH), 4.93 (d, J=7.8 Hz, IH), 4.57 (d, J=1.96 Hz, IH), 4.38 (dd, J=10.0, 6.44 Hz, IH), 4.31 (d, J=8.32 Hz, IH), 4.26 (d, IH), 4.21 (d, J=8.68 Hz, IH), 3.88 (d, J=6.96 Hz, IH), 2.50 (s, 3H), 2.44 (m, 2H), 2.32 (m, IH), 2.21 (t, J=8.28 Hz, 2H), 2.12 (m, IH), 1.91 (s, 3H), 1.90 (m, IH), 1.86 (s, IH), 1.78 (s, IH), 1.74 (s, 3H), 1.54 (m, 3H), 1.24 (m, 8H), 1.10 (s, 3H), 0.92 (t, 9H), 0.78 (t, 9H), 0.53 (m, 6H), 0.40 (m, 6H); FT-IR (neat) 3064.3, 3025.8, 2956.3, 1754.9, 1731.8, 1702.8, 1683.6, 1602.6, 1494.6, 1454.1, 1270.9, 1245.8 cm ^"1 ; MS (electrospray m/e 1034.4,

(M+H) ⁺ , 1056.4 (M+Na) ⁺ ; HPLC: C18 column, CH ₃ CN (40%-100%)/H ₂ O, RT:22.46 min, Chrom. purity: 95.44%.

B. Side Chain Cleavage to form 7-Et ₃ Si-10-DAB: 2 ml of methanol, 10 drops of mediylene chloride and 18 mg (10 eq) sodium borohydride was added to a 25 ml round bottomed flask, equipped witi a magnetic stir bar, having 56.9 mg (0.055 mmol) of 2',7- Bis(Et ₃ Si)-10-DAT. The reaction was stirred at room temperature for twenty hours and die solvent was removed under reduced pressure. The residue was redissolved in mediylene chloride and d e resulting organic solution was then extracted twice widi 20 ml methylene chloride/water (50:50 by volume). The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to a viscous oil as residue. The residue was purified using a silica gel column eluting with a 5% methanol/methylene chloride solution. The selected fractions were then evaporated to dryness under reduced pressure. The dry organic material was then recrystallized from methanol. 24.9 mg of 7-Et ₃ Si-10-DAB were recovered resulting in a 92.3% yield. The proton nuclear magnetic resonance spectrum (400Hz; deuterated chloroform; chemical shift in ppm; coupling constants J in Hz).

C. Deprotection of 7-EtjSi-10-DAB: The procedure set out in Example I above was followed.

EXAMPLE VI A. Preparationof2',7-Bis(Et3Si)-10-DATCFromtheCrudeMixtureWhich Contains 2.5% 10-DATC: 16.2 mg containing approximately 2.5% 10-DATC and 500 μ\ of pyridine (32 mg/ml) were introduced under a nitrogen atmosphere into a 25 ml round bottom flask equipped witii a magnetic stir bar. 4.7 ml (18-eq) of triethylsilyl chloride was added via a syringe. The mixture was then stirred at room temperature overnight. The mixture was then diluted with methylene chloride and extracted twice with 10% copper sulfate, water and brine. The organic phase was collected, dried over anhydrous magnesium sulfate and subsequently filtered. The solvent was removed under reduced pressure to obtain a colorless oil as a crude product. The crude product was purified by flash column chromatography or silica gel using air pressure and eluting with 25% ethylacetate/hexane. The selected fractions were evaporated to dryness wid a rotary evaporator under reduced pressure. 10.6 mg of 2',7-Bis(Et ₃ Si)-10-DATC were recovered.

B. Side Chain Cleavage to Form 7-EtaSi-lO-DAB: To a 4 ml vial, equipped with a magnetic stir bar, 10.6 mg of 2',7-Bis(Et ₃ Si)-10-DATC was added and dissolved in 0.5 ml MeOH. 8.2 mg of sodium borohydride was added and the reaction was stirred at room temperature overnight followed by removal of all solvent under reduced pressure. The residue was redissolved in methylene chloride and d e resulting organic solution was then extracted twice with water. The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to obtain 8.6 mg of 7-Et ₃ Si-10-DAB as a major product and die side chain as a by product. HPLC analysis indicated a chromatographic purity of 30.2% C. Deprotection of 7-Et ₃ Si-10-DAB: The above procedure for deprotection set out in Example HI above was followed.

EXAMPLE π A. Preparation of 2',7-Bis(Et ₃ Si)-10-DATC From the Crude Mixture Which Contains 7.5% 10-DATC: 21.9 mg containing approximately 7.5% 10-DATC and 700 μl of pyridine (31 mg/ml) were introduced under a nitrogen atmosphere into a 25 ml round bottom flask equipped widi a magnetic stir bar. 24.8 μl (22-eq) of triethylsilyl chloride was added via a syringe. The mixture was then stirred at room temperature overnight.

The mixture was then diluted witii methylene chloride and extracted twice with 10% copper sulfate, water and brine. The organic phase was collected, dried over anhydrous magnesium sulfate and subsequently filtered. The solvent was removed under reduced pressure to obtain a colorless oil as a crude product. The crude product was purified by flash column chromatography or silica gel using air pressure and eluting with 25% ethylacetate/hexane. The selected fractions were evaporated to dryness with a rotary evaporator under reduced pressure. 14.2 mg of 2',7-Bis(Et ₃ Si)-10-DATC were recovered. B. Side Chain Cleavage to Form 7-EtaSi-lO-DAB: To a 4 ml vial, equipped with a magnetic stir bar, 14.2 mg of 2',7-Bis(Et ₃ Si)-10-DATC was added and dissolved in 0.5 ml MeOH. 12.3 mg of sodium borohydride was added and the reaction was stirred at room temperature overnight followed by removal of all solvent under reduced pressure. The residue was redissolved in methylene chloride and the resulting organic solution was then extracted twice with water. The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to obtain 5.5 mg of 7-Et ₃ Si-10-DAB as a major product and the side chain as a by product. HPLC

analysis indicated a chromatographic purity of 70.3%

C. Deprotection of 7-Et ₃ Si-10-DAB: The above procedure for deprotection set out in

Example HI above was followed.

EXAMPLE m A. Preparation of 2',7-Bis(Et ₃ Si)-10-DAT: Prepared in the same manner as described above in Example I.

B. Side Chain Cleavage to Form 7-Et,Si-10-DAB: 29.4 mg (0.028 mmol) of 2',7- Bis(Et ₃ Si)-10-DAT and 640 l anhydrous metiianol were introduced into a 4 ml vial equipped with a magnetic stir bar. 8.8 mg (0.058 mmol, 2 equivalent) of cesium fluoride was added under a nitrogen atmosphere at room temperature. The mixture was then stirred for 5 days.

The reaction mixture was then diluted in ethyl acetate and the resulting organic solution was then extracted twice with 30 ml brine. The organic phase was collected and dried over anhydrous magnesium sulfate. The solvent was then evaporated under reduced pressure to obtain a crude product. The resulting major product proved to be identical to the 7-Et ₃ Si-10-DAB obtained in Example I above, as analyzed by TLC, MS and HPLC. Methyl ester side chain and 10-DAB were also found as by-products.

C. Deprotection of 7-Et ₃ Si-10-DAB: The above procedure for deprotection set out in Example I above was followed.