Title:
PREPARATION AND APPLICATION OF L-AMINO ACID DEAMINASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2021/184809
Kind Code:
A1
Abstract:
Provided are preparation and application of an L-amino acid deaminase mutant. A protein is transformed by analyzing a flexible Loop ring structure around a binding site of a pmirLAAD product, and finally, the optimal mutant pmirLAADM4 is obtained by means of experimental verification. The catalytic efficiency (1.61 mM-1 min-1) and a product inhibition constant (5.4 mM) are improved by 5.2 times and 5.7 times respectively compared with those of a contrast group; a yield of α-ketoisovaleric acid can reach 96.5 g/L, and a conversion rate is greater than 97%. An industrial process of producing the α-ketoisovaleric acid by an enzymatic conversion method is accelerated.
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Inventors:
WU JING (CN)
PEI SHANSHAN (CN)
LIU JIA (CN)
SONG WEI (CN)
CHEN XIULAI (CN)
LUO QIULING (CN)
PEI SHANSHAN (CN)
LIU JIA (CN)
SONG WEI (CN)
CHEN XIULAI (CN)
LUO QIULING (CN)
Application Number:
PCT/CN2020/130330
Publication Date:
September 23, 2021
Filing Date:
November 20, 2020
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N9/06; C12N1/21; C12N15/53; C12N15/70; C12P7/40
Foreign References:
| CN111269900A | 2020-06-12 | |||
| CN108841844A | 2018-11-20 | |||
| CN109321541A | 2019-02-12 | |||
| KR20090088630A | 2009-08-20 |
Other References:
QUAN ZHANG, WEI SONG, CAN ZHANG, SHANSHAN PEI, XIULAI CHEN, JIA LIU, QIULING LUO, LIMING LIU: "Recent Advances in Enzymatic Production of Alpha-Keto Acids", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 35, no. 7, 25 July 2019 (2019-07-25), pages 1193 - 1205, XP055851363, DOI: 10.13345/j.cjb.180455
WANG YUE , LI JIANG-HUA , DU GUO-CHENG , LIU LONG: "Molecular Modification of L-amino Acid Deaminase and Optimization of α-ketoglutaric Acid Production by Whole-cell Biocatalysis", CHINA BIOTECHNOLOGY, vol. 59, no. 3, 15 March 2019 (2019-03-15), pages 56 - 64, XP055851365, DOI: 10.13523/j.cb.20190308
WANG YUE , LI JIANG-HUA , DU GUO-CHENG , LIU LONG: "Molecular Modification of L-amino Acid Deaminase and Optimization of α-ketoglutaric Acid Production by Whole-cell Biocatalysis", CHINA BIOTECHNOLOGY, vol. 59, no. 3, 15 March 2019 (2019-03-15), pages 56 - 64, XP055851365, DOI: 10.13523/j.cb.20190308
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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