Title:
PREPARATION, CHARACTERIZATION AND USE OF RECOMBINANT MARTENTOXIN
Document Type and Number:
WIPO Patent Application WO/2020/094005
Kind Code:
A1
Abstract:
Provided is a method for preparing a recombinant Martentoxin (MarTX toxin for short), the method comprising: connecting a Martentoxin gene and a maltose binding protein with a vector pETDuent-1 to obtain a recombinant expression vector pETDuent-1-MarTX; converting the recombinant expression vector into E.coli Origami B (DE3), and by means of screening, obtaining a strain with a high-efficiency expression level; and by means of inducible expression and affinity chromatography purification, obtaining a recombinant MarTX toxin with a biological activity, which can significantly inhibit a large-conductance, calcium ion- and voltage-activated potassium ion (BK) channel current. Further provided is the use of the MarTX toxin in drug screening of a (α+β4) BK channel current inhibitor.
Inventors:
CAO CHUNYANG (CN)
LIU XINLIAN (CN)
TAO JIE (CN)
JI YONGHUA (CN)
LAN WENXIAN (CN)
LIU XINLIAN (CN)
TAO JIE (CN)
JI YONGHUA (CN)
LAN WENXIAN (CN)
Application Number:
PCT/CN2019/115709
Publication Date:
May 14, 2020
Filing Date:
November 05, 2019
Export Citation:
Assignee:
SHANGHAI INST ORGANIC CHEMISTRY CAS (CN)
International Classes:
C07K19/00; C12N1/21; C12N15/62; C12P21/06; C12Q1/02
Foreign References:
| CN109384852A | 2019-02-26 | |||
| CN104946677A | 2015-09-30 | |||
| CN102242125A | 2011-11-16 |
Other References:
CHEN, XIAOMING: "The Expression Optimization for the Martentoxin and the Struture Simulation of BK Channel Pore", MEDICINE&PUBLIC HEALTH, CHINA MASTER'S THESES FULL-TEXT DATABASE, 15 February 2016 (2016-02-15), XP055706332
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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