PREPARATION CONTAINING CELL EXTRACT FOR SYNTHESIZING CELL-FREE PROTEIN AND MEANS FOR SYNTHESIZING CELL-FREE PROTEIN

Title:

PREPARATION CONTAINING CELL EXTRACT FOR SYNTHESIZING CELL-FREE PROTEIN AND MEANS FOR SYNTHESIZING CELL-FREE PROTEIN

Document Type and Number:

WIPO Patent Application WO/2000/068412

Kind Code:

A1

Abstract:

A preparation for synthesizing a cell-free protein which contains a cell extract prepared by eliminating from an organism systems relating to the inhibition of self-protein synthesis reactions; an apparatus for synthesizing a cell-free protein provided with a reaction tank for synthesizing the cell-free protein; and a kit to be used therefor. The above preparation is obtained as a product which can be stored at room temperature while sustaining the biological functions of the cell extract. A means for continuously synthesizing a cell-free protein comprising a cell extract from which substances inhibiting self-protein synthesis reactions have been substantially eliminated, involving a procedure selected from among addition, preservation, exchange and discharge of a factor selected from among mRNA serving as a template in the synthesis reactions, an enzyme participating in the energy regeneration system, a substrate and an energy source.

Inventors:

ENDO YAETA (JP)
NISHIKAWA SHIGEMICHI (JP)

Application Number:

PCT/JP1999/004088

Publication Date:

November 16, 2000

Filing Date:

July 29, 1999

Export Citation:

Click for automatic bibliography generation Help

Assignee:

WAKENYAKU CO LTD (JP)
ENDO YAETA (JP)
NISHIKAWA SHIGEMICHI (JP)

International Classes:

C12M1/12; C12P21/02; (IPC1-7): C12P21/00; C12N15/10; C12M1/00

Domestic Patent References:

WO1998002532A1

1998-01-22

Foreign References:

JPH07203984A	1995-08-08
JPH1080295A	1998-03-31
JPH06503477A	1994-04-21
JPH07147992A	1995-06-13

Other References: