Title:
PREPARATION OF DNA-TEMPLATED GOLD NANOCLUSTER PROBE HAVING PRECISE NUMBER OF ATOMS, AND APPLICATION THEREOF IN SINGLE-CELL ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2023/092892
Kind Code:
A1
Abstract:
Preparation of a DNA-templated gold nanocluster probe having a precise number of atoms, and an application thereof. The preparation comprises: (1) designing a specific DNA sequence having a hairpin structure; (2) mixing a DNA solution with a gold salt solution to form a mixed solution; and (3) adding a dimethylamine borane (DMAB) reducing agent in a certain condition to enable the mixed solution system to experience an oxidation-reduction reaction, so that high-valence gold ions in the gold salt are reduced to gold atoms or gold ions, and gold is bonded to cytosine in the hairpin structure designed in the DNA to form a DNA-templated gold nanocluster. The gold nanocluster has good fluorescence and a precise number of atoms, ribonucleic acid splice variants can be visualized in situ by means of the fluorescence property, and single-cell ribonucleic acid splice variants can be quantified on laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) by means of the precise number of atoms property.
Inventors:
REN XIAOJUN (CN)
WANG SHIZHENG (CN)
GAO XUEYUN (CN)
WANG SHIZHENG (CN)
GAO XUEYUN (CN)
Application Number:
PCT/CN2022/080041
Publication Date:
June 01, 2023
Filing Date:
March 10, 2022
Export Citation:
Assignee:
UNIV BEIJING TECHNOLOGY (CN)
International Classes:
C09K11/06; C07H23/00; C09K11/58; G01N27/626
Domestic Patent References:
| WO2015114127A1 | 2015-08-06 | |||
| WO2017082517A1 | 2017-05-18 |
Foreign References:
| US20050048546A1 | 2005-03-03 | |||
| CN111991561A | 2020-11-27 | |||
| US6979729B1 | 2005-12-27 |
Other References:
SHEN JIA-JIA, CHEN WAN-TING, YA DING: "Preparation and applications of gold nanoclusters with precise atom number", ACTA PHARMACEUTICA SINICA, YAOXUE XUEBAO, CN, vol. 53, no. 9, 13 June 2018 (2018-06-13), CN , pages 1484 - 1492, XP093069205, ISSN: 0513-4870, DOI: 10.16438/j.0513-4870.2018-0350
LIU ZHONGDE, HUANG CHENG ZHI, WANG YI, ZHANG PU: "Oligonucleotide-stabilized silver nanoclusters as fluorescent probes for highly selective detection of thiol-containing drugs", ZHONGGUO KEXUE. HUAXUE - SCIENTIA SINICA CHIMICA, ZHONGGUO KEXUE ZAZHISHE, CN, vol. 41, no. 6, 1 June 2011 (2011-06-01), CN , pages 1037 - 1043, XP093069206, ISSN: 1674-7224, DOI: 10.1360/032010-777
LIU ZHONGDE, HUANG CHENG ZHI, WANG YI, ZHANG PU: "Oligonucleotide-stabilized silver nanoclusters as fluorescent probes for highly selective detection of thiol-containing drugs", ZHONGGUO KEXUE. HUAXUE - SCIENTIA SINICA CHIMICA, ZHONGGUO KEXUE ZAZHISHE, CN, vol. 41, no. 6, 1 June 2011 (2011-06-01), CN , pages 1037 - 1043, XP093069206, ISSN: 1674-7224, DOI: 10.1360/032010-777
Attorney, Agent or Firm:
BEIJING SIHAI TIANDA INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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