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Title:
PREPARATION OF MACROMOLECULAR POLYPEPTIDE BASED ON GASTROINTESTINAL DIGESTION AND METHOD FOR IN SITU REAL-TIME MONITORING PROCESS OF SAME
Document Type and Number:
WIPO Patent Application WO/2019/091471
Kind Code:
A1
Abstract:
Disclosed are the preparation of a macromolecular polypeptide based on gastrointestinal digestion and a method for in situ real-time monitoring the process of the same, relates to the fields of polypeptide preparation and online monitoring. In the preparation of the polypeptide, use a soluble technology to process a protein, add a specific protease to make the reaction system close to the optimal catalytic system, and maintain the reaction system to the predetermined degree of enzymatic hydrolysis, making the content of the protein that has not been enzyme hydrolyzed significantly decreased, the homogeneity of enzymatic hydrolysate high, rapid absorbability after gastrointestinal digestion and biological activity significantly improved. The degree of hydrolysis and the antihypertensive activity of the enzymatic hydrolysate after gastrointestinal digestion are monitored online during the preparation process, and the prediction model is very close to the actually measured model and can achieve an effective in situ detection.

Inventors:
MA HAILE (CN)
LI YUNLIANG (CN)
REN XIAOFENG (CN)
YANG XUE (CN)
HUANG SHANFEN (CN)
WANG YUCHENG (CN)
Application Number:
PCT/CN2018/114949
Publication Date:
May 16, 2019
Filing Date:
November 12, 2018
Export Citation:
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Assignee:
UNIV JIANGSU (CN)
International Classes:
C12P21/06; G01N21/359
Foreign References:
CN108004287A2018-05-08
CN107858390A2018-03-30
CN107858391A2018-03-30
CN108107018A2018-06-01
CN1552891A2004-12-08
CN104970183A2015-10-14
CN105628644A2016-06-01
CN103329992A2013-10-02
Other References:
LIU, ZHIGUO ET AL.: "Preparation of ACE Inhibitor from Enzyme-Degested Zein", FOOD RESEARCH AND DEVELOPMENT, vol. 27, no. 2, 31 December 2006 (2006-12-31), pages 138 - 141
WANG, KAI ET AL.: "Effect of Energy- Gathered Pulsed Ultrasonic Pretreatment on Enzymatic Hydrolysis of Corn Gluten Meal", JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY, vol. 17, no. 5, 31 December 2015 (2015-12-31), pages 127 - 133
ULUKO, H. ET AL.: "Effect of Power Ultrasound Pretreatment on Peptidic Profiles and Angiotensin Converting Enzyme Inhibition of Milk Protein Concentrate Hydrolysates", JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, vol. 94, no. 12, 10 January 2014 (2014-01-10) - 11 March 2014 (2014-03-11), pages 2420 - 2428, XP055607702, ISSN: 0022-5142, DOI: 10.1002/jsfa.6572
Attorney, Agent or Firm:
JW IP LAW FIRM (CN)
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