FIELD OF THE INVENTION

The present invention is in the field of care and protection of human health. In particular, the present invention is in the field of functional foods. In more particular, the present invention relates to the preparation for enhancing memory and treating dementia syndrome that can be made of medicinal herbs available in Vietnam.

BACKGROUND ART

Memory impairment (also known as confusion or absent-mindedness) is a progressive deterioration of memory and cognition due to the constant deterioration of the brain.

Memory impairment is a common condition in the elderly, because the nerve cells gradually degenerate over time. Nowadays, however, this is more and more seen in the youner people.

There are a number of daily manifestations to recognize each person's memory decline, for example:

- Often forgetting what have just been said, often forgetting the places of daily used items, events, often repeating the same stories;

- Being difficult of remembering new information, events and lessons;

- Lack of concentration in work and study;

- Often feeling tired, stressed, having sensitive mood, and being difficult of controlling behavior;

- Being difficult of concentrating in planning, solving puzzles or number calculations;

- Having difficulty in distinguishing timelines, seasons or locations;

- Impairing ability of judgment and decision making.

There are a lot of causes leading to such a memory impairment.

For young people, the first cause si stress. Stresses at work and study causes the body to produce many free radicals, which attack, damage, and even kill nerve cells and degenerate the brain. At that time, memory will gradually reduce and the functions of the brain will also be disturbed.

The second cause is not enough time for sleeping, i.e. frequent insomnia, making it difficult for the brain to drain "toxins" and strengthening long-term memory, leading to forgetfulness and slowness in daily activities and poorly solving problems.

The last cause is due to an unreasonable diet, high in sugar and fat, which makes the brain susceptible to "corrosion", reducing the ability to remember.

For the elderly, the first cause is the degeneration of the nervous system due to age. The second cause is diseases, wherein some diseases such as encephalitis, traumatic brain injury, stroke, lack of brain blood, etc., cause memory impairment. In general, the older you get, the more likely you are to face these conditions. Women after giving birth also have memory impairment that can be caused by hormonal imbalance, or depression, anxiety, insomnia for many different reasons. In addition, another cause is the body's lack of nutrients after giving birth.

Regardless of ages or genders, memory impairment also causes many consequences affecting health and the quality of life, reducing work and study efficiency, impairing intelligence leading to gradual loss of ability of thinking and self-care. This not only affects the quality of life of the patients, but also affects their families and society.

To reduce the memory impairment, the patients need to take combined measures such as: treatment of related diseases (if any), correction of psychological problems, lifestyle changes, scientific nutrition, risk factor control. Eliminating risk factors is very important for controlling disease progression, it is also very important for preventing from memory impairment, wherein adding an anti-free radical agent to control the condition of memory impairment is an effective and ongoing measure.

Free radicals are the leading cause of nerve cell damage and rapid degeneration. Studies have proven that adding natural anti-free radical agents can prevent from these toxic attacks on nerve cells, restoring the brain functions, thereby helping to restore brain function, thus improving the memory.

From the research results by the US scientists showed that, the natural biological active ingredients from blueberries and Ginkgo biloba have been shown to have free radical neutralizing effects. They have the ability to cross the blood-brain barrier, to nourish and protect nerve cells, increase blood to the brain, recover the functions of information recording in the brain for a smooth memorization, significantly improving memory impairment.

In addition to blueberries and Ginkgo biloba, there are many known medicinal herbs that are also beneficial for the brain, among them are Polyscias fruticosa (Polysciacis) roots, Stag's-hom clubmoss (Lycopodium clavatum) (Huperzia) sp.), Mucuna pruriens (Mucuna pruriens) seeds, dried nattokinase.

Polyscias fruticosa is considered the "ginseng for poor people", helping to increase the body's stamina and resistance, fighting fatigue, and helping appetite and sleeping well. Polyscias fruticosa has and effect of causing mild activation of neurons, increasing the amplitude of cortical voltage, increasing the amount of blood in the brain, thereby it is a good use for insomnia, vestibular disorders, memory impairment, Alzheimer's, tremors, etc..

Stag's-hom clubmoss (Lycopodium clavatum): The main active ingredient of Stag's-hom clubmoss (Lycopodium clavatum) is Huperzine A. This alkaloid can easily penetrate the blood brain barrier and act directly on brain neurons. This makes it easier for neural information to be communicated. Therefore, it responds very well to vestibular disorders, insomnia, Alzheimer's, Parkinson's, etc..

Mucuna pruriens contains the main ingredient as the precursor of dopamine that has the effect of restoring the nervous system, relieving the symptoms of dementia and preventing recurrence of neurological diseases such as vestibular disorders, loss of sleepping, etc..

Nattokinase is a fermented soybean product that helps prevent and dissolve blood clots. With the mechanism of dissolving the blood clots generated in the blood vessel walls, the enzymes in fermented soybeans will reduce the formation of blood clots, reduce the blockage of blood flow to the brain, and increase the blood circulation, helping to reduce the risk of diseases such as cerebrovascular accident, brain failure, memory impairment, stroke, etc. In fact, many kinds of brain-tonic and memory-enhancing preparations have been studied, prepared from the medicinal herbs mentioned above, separately or in combination. Typical for a product that combines medicinal herbs is a known product on the market with the trade name Nobel Tri Brain of the same owner. This is a combination product of 5 precious medicinal herbs including: Stag's-hom clubmoss (Lycopodium clavatum), Polyscias fruticosa, Mucuna pruriens, Ginkgo biloba and dried nattokinase with outstanding effects in the treatment of headache, dizziness, and erectile dysfunction, insomnia, memory impairment, reducing the symptoms of the disease and supporting the treatment of the disease at the root thanks to the mechanism of increasing neurotransmitters, increasing the amount of blood to the brain, reducing the formation of beta-amiloid plaques in the brain.

However, the preparations mentioned above are still limited in treating anemia, mental instability such as insomnia, heart palpitations, chest tightness, dizziness, that are common symptoms in the elderly, women after giving birth with memory impairment.

SUMMARY OF THE INVENTION

The purpose of the invention is to overcome the disadvantages mentioned above.

To this purpose, the present invention provides a the the preparation for enhancing memory and treating dementia syndrome wherein the addition of Medicinal leech (Hirudo medicinalis) has the effect of activating the blood, preventing the formation of blood clots, and especially a preparation consisting of 13 herbal medicines with good effects on blood loss, mental instability, insomnia, forgetfulness, heart palpitations, or bad sleep, used for cases of nervous weakness, hyperthyroidism causing dizziness, palpitations, rapid heartbeat, chest tightness.

These 13 herbs of the remedy include:

Rehmannia (Rehmannia Glutinosa) roots Rehmanniae glutinosae) clearing heat, cooling the body, helping to increase body fluid, and tonifying yin, to treat anemia, emaciation, paleness, nosebleeds, bleeding, menstrual bleeding, irregular menstruation, pregnancy, and coughing up blood. It is very effective in anti-inflammatory.

Semen Zizyphi jujubae (Ziziphi mauritibanae) has the effect of sedation, treatment of mental problems, insomnia, sweating, neurasthenia, long-term use can increase life expectancy.

Asparagus cochinchinensis roots {Asparagi cochinchinensis) has the effect of laxative, laxative, sputum, laxative. This is used to treat lung cancer, tuberculosis, cough, vomiting blood, heat illness, thirst (diabetes), new fluid loss, constipation.

Semen Thuya orientalis Platy cladi orientalis) coming to heart has the effect of mental health, coming to kidney has the effect of kidney tonic. Semen Thuya orientalis has the same effect as of Semen Zizyphi jujubae except Semen Thuya orientalis isneutral, its seeds have fat, benefit for heart.

Dongquai (Radix Angelicae sinensis) roots {Scrophulariae) has the effect of blood tonic.

Ophiopogon japonicus roots {Ophiopogonis japonica) has the effect of heart tonic, lung tonic, creasing new internal body fluid.

Schisandra chinensis fruits {Schisandrae) has the effect of sedation, kidney tonic. Dangshen (Radix Salviae militiorrhizae) roots (Salviae miltiorrhizae) has the effect of dissolving blood clots (stagnation of blood), generating new blood (post-neoplastic stasis), nourishing blood in pregnancy, regulating meridians.

Dangshen (Radix Salviae militiorrhizae) roots Codonopsis pilosulae) lung tonic and spleen tonic, liver detoxing if used in summer. It can be used instead of ginseng to treat spleen damage, fatigue, weak limbs, and cough.

Polygala tenuifolia roots (Polygalae) is used to treat cough, reduce phlegm, treat bronchitis, forgetfulness, memory loss, impotence, weakness, dreams, tonic for men and the elderly, medicine to brighten eyes and ears due to effects on the whole body. It also cures chest pain, tuberculosis, poor sleep, neurasthenia, nightmares.

Figwort (Scrophulariaceae) roots (Scrophulariae) helps cooling body, creasing new internal body fluid, good for heart.

Poria cocos wolf fruits (Poria) has the effect of sedation, heart tonic.

Platycodon grandiflorus roots (Platycodi grandiflori) has the effect of lung tonic, heart tonic and blood tonic.

In particular, the preparation of the present invention as in the Claim 1 has ingredients comprising:

Stag’s-hom clubmoss (Lycopodium clavatum) powder (Huperzia sp.)

Extract powder of herbs selected from: Rehmannia (Rehmannia Glutinosa) roots (Rehmanniae glutinosae), Semen Zizyphi jujubae (Ziziphi mauritibanae) , Asparagus cochinchinensis roots (Asparagi cochinchinensis) , Semen Thuya orientalis (Platy cladi orientalis), Dongquai (Radix Angelicae sinensis) roots (Scrophulariae), Ophiopogon japonicus roots (Ophiopogonis japonica), Schisandra chinensis fruits (Schisandrae), Dangshen (Radix Salviae militiorrhizae) roots Salviae miltiorrhizae), Dangshen (Radix Salviae militiorrhizae) roots (Codonopsis pilosulae), Polygala tenuifolia roots (Polygalae), Figwort (Scrophulariaceae) roots (Scrophulariae), Poria cocos wolf fruits (Poria), Platycodon grandiflorus roots (Platycodi grandiflori),'

Extract powder of Mucuna pruriens seeds (Mucuna pruriensfl

Extract powder of Medicinal leech (Hirudo medicinalis);

Extract of Polyscias fruticosa (Polysciacis),'

Extract of Ginkgo biloba (Folium Ginko bilobafl

Dried nattokinase; and

Preservatives.

The preperation is manufactured by a method comprising the following steps:

Preparing the extract powder of herbs as follows:

A mixture of the medicinal herb comprising Rehmannia (Rehmannia Glutinosa) roots, Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, Platycodon grandiflorus roots that have met the medically required standard are thoroughly washed, put in an extraction pot. Filled with water 10-15 cm above the surface of the medicinal herbs and boiled for 3 hours, then a first extract is collected out and the same process is repeated more two times to obtain a second and third extracts. 3 said obtained extracts are combined, filtered through a coarse cloth, then concentrated until obtaining an extract paste having its moisture content of about 20%. Adding natri benzoat dissolved in etanol 96% is added until enough, followed by stirring well. The obtained extract paste is poured into a mould to obtain an extract cake. Said extract cake is cut into slices with a thickness of about 3-5 cm, then said thick slices are spreaded on a tray for drying in a stationaray oven, heat-drying cao at a temperature of about 85 °C until dryness, then let be cool and finely ground by hammer mill, then sieved through a 0.2mm sieve 0,2 mm, to obtain extract powder of herbs (having its moisture content of < 5%) for use to prepare the preparation.

Preparing the extract powder of Stag’s-hom clubmoss (Lycopodium clavatum), Mucuna pruriens plant and Medicinal leech (Hirudo medicinalis) bang each riia sach a mixture of Stag’s- hom clubmoss (Lycopodium clavatum)/seeds of Mucuna pruriens plant/Medicinal leech (Hirudo medicinalis) that have met the medically required standard, then dried until having a moisture content of less than 9%, ground into fine powder, then sieved through a 0.2mm sieve.

Preparing an extract powder of Polyscias fruticosa separately in the same way as the steps of preparing the extract powder of herbs mentioned above.

Preparing an extract powder of Ginkgo biloba separately in the same way as the steps of preparing the extract powder of herbs mentioned above, wherein the extraction solvent is a mixture of ethanol in water 40-50%.

Finally, mixing all the extract powder of herbs, extract powder of Stag’s-hom clubmoss (Lycopodium clavatum), Mucuna pruriens plant and Medicinal leech (Hirudo medicinalis), an extract powder of Polyscias fruticosa, an extract powder of Ginkgo biloba obtained from the above said preparing steps with nattokinase powder in a proportion appropriately and according to the principle of double powder mixing to obtain an ingredient mixture of the preparation for enhancing memory and treating dementia syndrome.

In one preferred embodiment as mentioned in claim 2, the preparation of the present invention further comprises excipient is mixture of talc, magie stearat and starch for encapsulating the preparation and pressing into tablets.

In one preferred embodiment as mentioned in claim 3, the herbal material for preparing the extract powder of herbs consists of ingredients as follows (% by weight in comparision with the total amount of the berbal material):

Rehmannia (Rehmannia Glutinosa) roots: 10-50;

Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, each: 1-15;

Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, Platycodon grandiflorus roots, each: 0,5-10.

In one preferred embodiment as mentioned in claim 4, the herbal material for preparing the extract powder of herbs consists of ingredients as follows (% by weight in comparision with the total amount of the berbal material):

Rehmannia (Rehmannia Glutinosa) roots: 15-45; Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, each: 2-13;

Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, Platycodon grandiflorus roots, each: 1-8.

In one preferred embodiment as mentioned in claim 5, the herbal material for preparing the extract powder of herbs consists of ingredients as follows (% by weight in comparision with the total amount of the berbal material):

Rehmannia (Rehmannia Glutinosa) roots: 20-40.

Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, each: 4-11.

Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, Platycodon grandiflorus roots, each: 2-6.

The extract powder then are mixed together with adding nattokinase powder and preservatives at a predetermined ratio to obtain the preparation of the present invention.

In one preferred embodiment as in claim 6, the ingredient quantification of the preperation (% by weight in comparision with the amount of the preparation) is as follows:

Stag’s-hom clubmoss (Lycopodium clavatum) powder: 10-75, more preferably 15-70, most preferably 17-67;

Extract powder of herbs: 2-20, more preferably 5-18, most preferably 6-15;

Extract powder of Mucuna pruriens seeds: 0,1-20, more preferably 2-17, most preferably 3-15;

Extract powder of Medicinal leech (Hirudo medicinalis): 0,1-20, more preferably 2-17, most preferably 3-15;

Extract of Polyscias fruticosa: 5-25, more preferably 7-22, most preferably 8-20;

Extract of Ginkgo biloba: 5-15, more preferably 7-12, most preferably 8-11;

Nattokinase powder: 1-20, more preferably 2-17, most preferably 3-15; and

Preservatives (natri benzoat): 0,01-0,3, more preferably 0,05-0,25, most preferably 0,1- 0,2.

The preparation of the present invention is obtained in powder form. The obtained powder can be used to prepare several medicine forms such as capsules or syrup for oral administration or the other forms. For preparing capsules from the powder of the preparation, suitable excipients are added at a predetermined ratio, depending on dosage. In one preferred embodiment the excipients is a mixture comprising talc, magie stearat and starch. After mixing with the excipients, the preparation is pressed into capsules for end product as tablets.

To prepare a syrup preparation, it is necessary to dissolve the powdered preparation in a suitable liquid excipient to obtain a convenient syrup for oral administration. BRIEF DESCRIPTION OF THE DRAWINGS

Fig. 1 is Chart 1 of the effect of capsules on time the mouse can find the target cavity in day 8.

Fig. 2 is Chart 2 of the effect of the Capsule on the time the mouse stays on the axis of rotation

DETAILED DESCRIPTION OF THE INVENTION

The invention is described in detail in the following preferred embodiments. It should be understood, however, that these alternatives are for the purpose of clarifying the invention and not for limiting the scope of the invention. In particular, the preparation for enhancing memory and treating dementia syndrome made from herbs of present invention comprising ingredients extracted from medicinal herbs as mentioned below along with the way of preparation.

Stag’s-hom clubmoss (Lycopodium clavatum) powder

This is the main ingredient of the preparation. Stag’s-hom clubmoss (Lycopodium clavatum) powder is prepared by thoroughly washing a mixture of Stag’s-hom clubmoss (Lycopodium clavatum) that have met the medically required standard, then drying until a moisture content of less than 9%, then grinding into fine powder that finally sieving through a 0.2mm sieve.

Extract powder of herbs

Extract powder of herbs is prepared from 13 ingredients comprising: Rehmannia (Rehmannia Glutinosa) roots, Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, and Platycodon grandiflorus roots. These materials are selected so that they meet the medically required standard, then thoroughly cleaned, mixed together at a predetermined ratio then put in an extraction pot, filled with water 10-15 cm above the surface of the medicinal herbs and boiled for 3 hours. After that, the first extract is collected, then the same process is repeated for 2 further times to collect the second and the third extracts. These 3 obtained extracts are combined, then filtered through a coarse cloth, then concentrated until obtaining an extract paste having its moisture content of about 20%. Natri benzoat dissolved in etanol 96% is added until enough, followed by stirring well. The obtained extract paste is poured into a mould to obtain an extract cake. Said extract cake is cut into slices with a thickness of about 3-5 cm, then spreaded on a tray for drying in a stationaray oven at a temperature of about 85 °C until complete dryness, then let be cool and finely ground by hammer mill, then sieved through a 0.2mm sieve to obtain extract powder of herbs (having its moisture content of < 5%) for use to prepare the preparation.

More preferably, the mixture the herbal material for preparing the extract powder of herbs consists of ingredients as follows (% by weight in comparision with the total amount of the berbal material): Rehmannia (Rehmannia Glutinosa) roots: 10-50; Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, each: 1-15; Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, Platycodon grandiflorus roots, each: 0,5-10.

Further more preferably mixture the herbal material for preparing the extract powder of herbs consists of ingredients as follows (% by weight in comparision with the total amount of the berbal material): Rehmannia (Rehmannia Glutinosa) roots: 15-45; Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, each: 2-13; Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, Platycodon grandiflorus roots, each: 1-8.

Most preferably mixture the herbal material for preparing the extract powder of herbs consists of ingredients as follows (% by weight in comparision with the total amount of the berbal material): Rehmannia (Rehmannia Glutinosa) roots: 20-40; Semen Zizyphi jujubae, Asparagus cochinchinensis roots, Semen Thuya orientalis, Dongquai (Radix Angelicae sinensis) roots, Ophiopogon japonicus roots, Schisandra chinensis fruits, each: 4-11; Dangshen (Radix Salviae militiorrhizae) roots, Dangshen (Radix Salviae militiorrhizae) roots, Polygala tenuifolia roots, Figwort (Scrophulariaceae) roots, Poria cocos wolf fruits, Platycodon grandiflorus roots, each: 2- 6.

Extract powder of Mucuna pruriens seeds

The extract powder of Mucuna pruriens plant is prepared in the same way as for Stag’s-hom clubmoss (Lycopodium clavatum) powder. Seeds of Mucuna pruriens plant that have met the medically required standard is thoroughly washed, then dried until having a moisture content of less than 9%, ground into fine powder, then sieved through a 0.2mm sieve to obtain extract powder of Mucuna pruriens plant.

Extract powder of Medicinal leech (Hirudo medicinalis)

The extract powder of Medicinal leech (Hirudo medicinalis) is also prepared in the same way as for Stag’s-hom clubmoss (Lycopodium clavatum) powder. Medicinal leech (Hirudo medicinalis) that have met the medically required standard is thoroughly washed, then dried until having a moisture content of less than 9%, ground into fine powder, then sieved through a 0.2mm sieve to obtain extract powder of Medicinal leech (Hirudo medicinalis).

Extract powder of Polyscias fruticosa

The extract powder of Polyscias fruticosa is prepared in the same way as for extract powder of herbs as mentioned above.

Extract powder of Ginkgo biloba

The extract powder of Ginkgo biloba is prepared in the same way as preparing the extract powder of herbs mentioned above, except the extraction solvent is a mixture of etanol in water 40-50%.

After having prepared the medicinal extract powder as mentioned above, all the extract powder of Stag’s-horn clubmoss (Lycopodium clavatum), the extract powder of herbs, the extract powder Mucuna pruriens plant, Medicinal leech (Hirudo medicinalis) powder, the extract powder of Polyscias fruticosa, the extract powder of Ginkgo biloba vdi dried nattokinase and preservatives of natri benzoat are mixted in a proportion appropriately and according to the principle of double powder mixing to obtain an ingredient mixture of the preparation for enhancing memory and treating dementia syndrome.

More preferably, these ingredients of the preparation of the present invention are (% by weight in comparision with the amount of the preparation) as follows: Stag’s-hom clubmoss (Lycopodium clavatum) powder: 10-75; extract powder of herbs: 2-20; extract powder of Mucuna pruriens seeds: 0,1-20; extract powder of Medicinal leech (Hirudo medicinalis): 0,1-20; extract of Polyscias fruticosa: 5-25; extract of Ginkgo biloba: 5-15; nattokinase powder: 1-20 and natri benzoat: 0,01-0,3.

Further more preferably, these ingredients of the preparation of the present invention are (% by weight in comparision with the amount of the preparation): Stag’s-hom clubmoss (Lycopodium clavatum) powder: 15-70; extract powder of herbs: 5-18; extract powder of Mucuna pruriens seeds: 2-17; extract powder of Medicinal leech (Hirudo medicinalis): 2-17; extract of Polyscias fruticosa: 7-22; extract of Ginkgo biloba: 7-12; nattokinase powder: 2-17 and natri benzoat: 0,05- 0,25.

Most preferably, the ingredients of the preparation of the present invention are (% by weight in comparision with the amount of the preparation): Stag’s-horn clubmoss (Lycopodium clavatum) powder: 17-67; extract powder of herbs: 6-15; extract powder of Mucuna pruriens seeds: 3-15; extract powder of Medicinal leech (Hirudo medicinalis): 3-15; extract of Polyscias fruticosa: 8- 20; extract of Ginkgo biloba: 8-11; nattokinase powder: 3-15 and natri benzoat: 0,1 -0,2.

The preparation of the present invention is obtained in powder form. The obtained powder can be used to prepare several medicine forms such as capsules or syrup for oral administration or the other forms. For preparing capsules from the powder of the preparation, suitable excipients are added at a predetermined ratio, depending on dosage. In one preferred embodiment the excipients is a mixture comprising talc, magie stearat and starch. After mixing with the excipients, the preparation is pressed into capsules for end product as tablets.

To prepare a syrup preparation, it is necessary to dissolve the powdered preparation in a suitable liquid excipient to obtain a convenient syrup for oral administration.

Examples for implementation of the invention a) Preparing examples of the preparation (% by weight)

The ingredients in each example are mixed together, then extracted in the same way as mentioned in the DETAILED DESCRIPTION OF THE INVENTION. b) Preparing examples of the present invention

The ingredients of each example in the above table are mixed together under the principle of double powder mixing as mentioned in the part DETAILED DESCRIPTION OF THE INVENTION to obtain an ingredient mixture of the preparation for enhancing memory and treating dementia syndrome. c) Examples of preclinical study on the efficacy of the preparation of the present invention

Studies on memory improvement were conducted on 3 models: Morris Water Maze, Multi-T Maze, and Rotarod Performance Test and were conducted with capsules containing 800 mg of the preparation of the invention as shown in Example 13.

Example 1: Study on Morris Water Maze model

The memory improvement effect of capsules was evaluated on the Morris Water Maze model by the indicators as follows:

- Learning stage (days 1-5): The period of time for the mice found out harbor.

- Official assessment stage (day 6): Percentage of time for the mice swam within 1/4 tank where there is the harbor, percentage of distance for the mice swam within 3/4 tank where there is not the harbor. i) Learning stage Table 1. Effects of the capsules on the period of time for the mice found out the harbor

The results in Table 1 showed that:

- The biological control group: The period of time for finding out the harbor reduced after each training day, the difference was statistically significant on days 3, 4 and 5 in comparision with day 2 (the first day of the exercise of not sighting the harbor) (p<0.01 and 0.001).

- Model group using scopolamin: The period of time for finding out the harbor reduced with a difference in comparision with day 2 on all days 3, 4, 5 (p<0.05 and 0.01). Comparing with the biological control group showed that the group using scopolamin prolonged the period of time for finding out the harbor on all days tu 1-5, the difference is statistically significant (p<0.05).

- Positive control group orally taking donepezil and 2 groups orally taking the capsules with dosage of 0.48 capsules/kg (group 4) and 1.44 capsules/kg (group 5): The period of time for finding out the harbor reduced after each training day in comparision with day 2, the difference is statistically significant from day 3 (p<0.05; 0.01 and 0.001). In all study days, the period of time for finding out the harbor was significantly shortened in comparision with the model group, the difference is statistically significant (p<0.05). - There was no difference in the period of time for finding out the harbor in 2 groups using the capsules at the study time (p>0.05). ii) Official assessment stage

Table 2. Effects of the capsules on time % for the mice swam within 1/4 where there is the harbor

*p<0.05 in comparision with the model group

The results in Table 2 showed that, in the groups orally taking capsules, time % the mice swam within 1/4 tank where there is the harbor tended to last longer in comparision with the model group, wherein a statistically significant elongation was seen within the group orally taking the capsules with dosage of 1.44 capsules/kg (p<0.05).

Table 3. Effects of the capsules on % distance the mice swam within 3/4 tank where there is not the harbor

The results in Table 3 showed that, in the groups orally taking capsules, % distance the mice swam within 3/4 tank where there is not the harbor tended to reduce in comparision with the model group, a statistically significant reduce was seen within the group orally taking the capsules with dosage of 1.44 capsules/kg (p<0.05).

Example 2: Study on Multi-T Maze model The memory improvement effect of the capsules to be evaluated on the Multi-T Maze model was divided into 2 stages: learning (day 1-5) and official assessment (day 8) by the indicators: time, distance the mice found out the target cavity. i) Learning stage

Table 4. Effects of the capsules on the period of time for the mice found out the target cavity

*p<0.05; **p<0.01; *** p<0.001 in comparision with the model group p<0.05; A p<0.01; AAA p<0.001 in comparision with day 2

The results in Table 4 showed that:

- The biological control group: The mice had an improvement after the training days, in comparision with day 2 the period of time for finding out the the target cavity reduced, the difference is statistically significant from day 3 (p<0.05).

- Model group using scopolamin: The period of time for finding out the the target cavity with no difference from the first day in all study days (p>0.05). In comparision with the the biological control group, the group using scopolamin prolonged the period of time for finding out the the target cavity on all days of study, the difference is statistically significant (p<0.01 and 0.001).

- Positive control group using donepezil: The period of time for finding out the the target cavity was gradually reduced in all days of study in comparision with day 2 (p<0.01), and shorten in comparision with the model group in all days of study, the difference is statistically significant on days 1, 4 and 5 (p<0.05). - Group using the capsules with a dosage of 0.48 capsules/kg: In comparision with day 2, the period of time for finding out the the target cavity was significantly reduced on day 5, the difference is statistically significant with p<0.01. In comparision with the model group, the period of time for finding out the the target cavity of the mice tended to be shorten at all study point of time, the difference is statistically significant on day 5 of study (p<0.01).

- Group using the capsules with a dosage of 1.44 capsules/kg: In comparision with day 2, the period of time for finding out the the target cavity markedly reduced on day 5, the difference is statistically significant with p<0.05. In comparision with the model group, the period of time for finding out the the target cavity of the mice significantly reduced at all time points of study (p<0.05 and 0.01). ii) Official assessment stage

Fig. 1 is Chart 1 showing the effects of the capsules on period of time for finding out the the target cavity trong day 8

The results from Chart 1 showed that:

- In the official assessment stage, time and distance for the mice found out the target cavity in the model group using scopolamin was significantly longer in comparision with the the biological control group (p<0.01).

- Group orally taking donepezil has the period of time for the mice found out the target cavity significantly shortened in comparision with the model group (p<0.05).

- The period of time for the mice found out the target cavity in 2 groups orally taking capsules tended to reduce in comparision with the model group, a more significantly reduced levels were shown at a capsule dosage of 1.44 capsules/kg (p<0.001).

- The period of time for the mice found out the target cavity within the group orally taking the capsules with dosage of 1.44 capsules/kg is shorter in comparision with donepezil dosage of 2.4 mg/kg (p<0.001).

Table 5. Effects of the capsules on length of distance the mice traveled to reach the target cavity The results in Table 5 showed that, in the groups orally taking capsules, all lenghs of distances the mice need to travel to reach the target cavity were significantly reduced in comparision with the model group (p<0.05).

Example 3: Study on model Rotarod performance test

The memory improvement effect of the capsules on the rotarod performance test model was evaluated by the indicators of time the mice stayed on the rotarod.

Fig. 2 shows Chart 2 of the effects of the capsules on time the mice staying on the rotarod

The results tir Chart 2 showed that: i) Learning stage: The time the mice d tren the rotarod in all groups show no statistically significant difference in comparision with the model group (p>0.05). ii) Official assessment stage:

- Model group: The time the mice stayed on the rotarod show no statistically significant difference in comparision with learning stage (p>0.05).

- The biological control group and the group orally taking donepezil: The time the mice stayed on both the rotarod increased in comparision with the model group, and in comparision with learning stage, the difference is statistically significant (p<0.01 and 0.001).

- Group orally taking capsules: The time the mice stayed on the rotarod intended to increase in comparision with the model group, and in comparision with learning stage, however the difference is not statistically significant (p>0.05).

Conclusion

The study results on the memory improvement effect of capsules at dosages of 0.48 capsules/kg/day and 1.44 capsules/kg/day showed that:

- For Morris Water Maze model: Capsules at both dosages orally taken for 6 consecutive days clearly showed improving the ability of learning and memory on memory-impaired-by- scopolamine white mice as follows:

Learning stage: reduces the time to find out the harbor.

Official assessment stage: increases time % the mice swam within 1/4 tank where has the harbor as well as reduces distance % the mice swam within 3/4 tank where has no harbor.

- For Multi-T Maze model: Capsules at both dosages orally taken for 8 consecutive days were effective in improving the ability of learning and memory in memory-impaired-by- scopolamine white mice, shown in reducing time and distance for finding out the target cavity in comparision with the model group, wherein the effect is more clearly shown he within the group orally taking the capsules with dosage of 1.44 capsules/kg, this statistically significant difference was clearly seen in both indicators of the study.

- For model Rotarod performance test: Capsules at both dosages study orally taken for 7 consecutive days did not show improvement in learning and memory on memory-impaired-by- scopolamine white mice. d) Examples of clinical studies on the efficacy of the preparation of the present invention

The studies were conducted for 30 patients diagnosed with cerebral circulatory insufficiency, memory impairment who were treated with the capsules of the present invention. i) General characteristics of the study group:

- The average age of the study group is 56.28±9.2 years old, the eldest is 73 years old years old, the youngest is 46 years old. In which, men account for a highter proportion than women (63% male, 37% female)

- Occupation: The most common in this study were pensioners -11 patients (36.7%) and intellectuals were 10 patients (33.3%). Amongs the pensioners, teachers were the majority. ii) The effect of the composition (the capsules) on the clinical symptoms:

- Use of the capsules at a dose of 3 capsules /day, orally taking continuously for 3 months clearly showed:

Effects on headache symptom:

Using the capsules at a dose as mentioned above to study the effects on headache manifestations in headache nature, frequency of pain occurrence, pain prolongation found that 100% of the patients with cerebral circulatory insufficiency had headache, pain radiating all over the head. After 3 months of the treatment, these symptoms were clearly reduced to only 4 patients left (13.4%), showing that the drug works very well with this symptoms.

Effects on vertigo symptom:

Before treatment, 30 patients (100%) presented with dizziness. After treatment, there were only 6 patients (20%) still had the symptom.

Effects for memory:

The study assessed the expression of memory impairment: forgetfulness, transient forgetfulness and poor concentration, found that all the patients who had showed memory impairment after using the drug reduced to only 5 patients (16.6%) left. This can be explained by the composition of the invention consisting of Stag’s-hom clubmoss (Lycopodium clavatum), Polyscias fruticosa, Ginkgo biloba which have particularly good effects on cerebral circulation, thus increasing memory and concentration.

Effects on sleep disorder:

The study evaluating the manifestations of sleep disorders such as: prolonged insomnia, sleep rhythm disturbances and frequent somnolence, found that for all patients with cerebral circulatory insufficiency who had presented with sleep disturbances, after using the capsules reduced to only 8 patients (26.6%). This can be explained that the composition consists of Polyscias fruticosa, Ginkgo biloba, so the brain's circulatory ability, anti-aging and free radical scavenging, brain parenchymal damage, vestibular function and hearing after treatment has been significantly improved, so the symptoms of headache and dizziness are significantly reduced..

Effects on attention (concentration) disorder:

Manifestations of attention disorders are studied based on signs such as difficulty transferring attention from one object to another, mental stagnation, slow recognition of stimuli, and forgetfulness. Before taking the capsules, 10 patients (33.3%) showed signs of forgetfulness. After using the capsules, it was reduced to only 2 patients (6.7%). There were 8 patients (26.7%) whose attention were difficult, after the treatment there were only 3 patients (10%). The receiption of slow stimulation, mental depression also clearly decreased after using the capsules.

Effects on emotional disorder.

In the study, 14 patients (46.7%) showed emotional disturbances with signs such as restlessness, anxiety, and inability to control themselves. Signs of restlessness in 8 patients (26.7%), after treatment only 2 patients (6.7%) still had the symptom. Signs of not being able to control themselves of 6 patients (20%), after the treatment only 1 patient left (3.3%).

Effects on personality changes:

There were 12 patients (40%) showing personality changes such as mood swings (7 patients - 23.4%), or quarrelsome (5 patients - 16.6%). Most of these patients after using the capsules have improved significantly. iii) Study of effects on subclinical symptoms

Effects on blood biochemical indicators:

All the studied patients had a biochemical blood lipid test before treatment, found that most of the patients had a high blood fat indicator, especially the age group of 60 and older had a high total cholesterol indicator. After 3 months of treatment with the capsules, their blood lipids returned to normal limits, indicating that the capsules of the invention has an effect on reducing blood fat.

Effects on X-ray symptoms:

Study of X-ray of the cervical spine was conducted to find out the cause of cerebral circulatory insufficiency for 30 patients before using the capsules of the invention. After 3 months of using the capsules, it is found no difference between the images before and after the treatment. That proves that the capsules of the invention has no effect on changing the image of bone and joint damage of the cervical spine (joint stenosis, spinal stenosis, disc stenosis, etc.).

Effects on electroencephalogram:

The EEG recording conducted for 30 study patients using the capsules of the invention, the results showed that most of the patients showed cerebral hypoxia, the alpha and beta waveforms were still normal, but for alpha waves the frequency increases to an average of 63± 5.35 Hertz, (normally 8 to 13 waves/second). Beta waves usually have a fast frequency when ischemic, beta waves increase faster than normal (MHz), the amplitude of the waves is low, when ischemia is lower. After treatment, the amplitude and frequency of alpha and beta waves returned to the basal rhythm. This proves that the drug has increased cerebral blood circulation.