Title:
PREPARATION METHOD AND APPLICATION OF SEA CUCUMBER POLYSACCHARIDE
Document Type and Number:
WIPO Patent Application WO/2022/067774
Kind Code:
A1
Abstract:
A preparation method of a sea cucumber polysaccharide, comprising the following steps: (1) taking dry sea cucumbers, pulverizing the dry sea cucumbers into coarse particles, adding water to conduct enzymatic hydrolysis, performing centrifugation and taking a supernatant, and concentrating the supernatant to 1/5 of an original volume under a reduced pressure, and then performing centrifugation again (under the same centrifugation conditions as above) and taking a supernatant; subjecting the supernatant to three times of salting out and alcohol precipitation, then washing the precipitation twice with absolute ethanol, and evaporating the ethanol to obtain a crude sea cucumber polysaccharide; (2) dissolving the crude sea cucumber polysaccharide in pure water, loading the solution into an anion chromatography column (OH -), eluting the anion chromatography column with pure water and NaCl solutions with gradient concentrations, and collecting an NaCl eluent; and (3) subjecting the NaCl eluent to ultrafiltration for desalination, and concentrating the ultrafiltered retentate under a reduced pressure and freeze-drying the concentrate, to give the sea cucumber polysaccharide. The sea cucumber polysaccharide can be used to prepare anti-hyperlipidemia, anti-oxidation, anti-coagulation, anti-tumor, immunity-enhancing drugs and the like or healthcare products, has the effect of preventing thrombosis, and has a good auxiliary preventive effect for the elderly and people with high thrombosis risks.
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Inventors:
LI ZHENGUO (CN)
ZHENG SHUNLIANG (CN)
LI WEI (CN)
HU JIEWEN (CN)
NAN ZHIYUAN (CN)
ZHOU JIANBO (CN)
GUO YUQIAN (CN)
LI CHANGJIANG (CN)
ZHANG KAI (CN)
XIA KE (CN)
ZHENG SHUNLIANG (CN)
LI WEI (CN)
HU JIEWEN (CN)
NAN ZHIYUAN (CN)
ZHOU JIANBO (CN)
GUO YUQIAN (CN)
LI CHANGJIANG (CN)
ZHANG KAI (CN)
XIA KE (CN)
Application Number:
PCT/CN2020/119685
Publication Date:
April 07, 2022
Filing Date:
September 30, 2020
Export Citation:
Assignee:
MUDANJIANG YOUBO PHARMACEUTICAL CO LTD (CN)
JIUZHITANG CO LTD (CN)
HAINAN JIUZHITANG PHARMACEUTICAL CO LTD (CN)
JIUZHITANG CO LTD (CN)
HAINAN JIUZHITANG PHARMACEUTICAL CO LTD (CN)
International Classes:
A61P3/06; A61K35/616; A61P7/02; A61P35/00; A61P37/04; A61P39/06; C08B37/00
Foreign References:
| CN110437288A | 2019-11-12 | |||
| CN102367285A | 2012-03-07 | |||
| CN102443077A | 2012-05-09 |
Other References:
SU WEI, XUE CHANGHU, CUI HONGBO, CHANG YAOGUANG: "Purification and Diversity Analysis of Chemical Composition of Polysaccharides from Three Kinds of Sea Cucumbers", JOURNAL OF CHINESE INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CN, vol. 12, no. 8, 31 August 2012 (2012-08-31), CN , pages 215 - 220, XP055920460, ISSN: 1009-7848, DOI: 10.16429/j.1009-7848.2012.08.033
ZHANG JIAN, LIU FANG;ZHANG JIN-HAO;LIU SHAO-WEI;ZHENG LI-YONG;ZHAO YUN-PING;WANG GONG-MING;JING YUE-XIN;QIAO RUI-GUANG: "Research Progress on Preparation, Chemical Analysis and Biological Activity of Sea Cucumber Polysaccharide", JOURNAL OF FOOD SAFETY AND QUALITY, vol. 9, no. 8, 30 April 2018 (2018-04-30), pages 1730 - 1736, XP055920464
USTYUZHANINA NADEZHDA E, BILAN MARIA I, DMITRENOK ANDREY S, SHASHKOV ALEXANDER S, KUSAYKIN MIKHAIL I, STONIK VALENTIN A, NIFANTIEV: "Structure and biological activity of a fucosylated chondroitin sulfate from the sea cucumber Cucumaria japonica", GLYCOBIOLOGY, OXFORD UNIVERSITY PRESS, US, vol. 26, no. 5, 1 May 2016 (2016-05-01), US , pages 449 - 459, XP055920465, ISSN: 0959-6658, DOI: 10.1093/glycob/cwv119
WU JINJIN, XIA CONGHUA, GE WEIHONG: "Study on Anion Exchange Gum Purification of Radix Actinidia Chinensis Polysaccharides", ZHEJIANG ZHONGYIYAO DAXUE XUEBAO - JOURNAL OF ZHEIJIAN COLLEGE OF TCM, ZHEJIANG ZHONGYIYAO DAXUE, CN, vol. 34, no. 6, 30 November 2010 (2010-11-30), CN , pages 906 - 910, XP055920467, ISSN: 1005-5509, DOI: 10.16466/j.issn1005-5509.2010.06.032
ZHANG JIAN, LIU FANG;ZHANG JIN-HAO;LIU SHAO-WEI;ZHENG LI-YONG;ZHAO YUN-PING;WANG GONG-MING;JING YUE-XIN;QIAO RUI-GUANG: "Research Progress on Preparation, Chemical Analysis and Biological Activity of Sea Cucumber Polysaccharide", JOURNAL OF FOOD SAFETY AND QUALITY, vol. 9, no. 8, 30 April 2018 (2018-04-30), pages 1730 - 1736, XP055920464
USTYUZHANINA NADEZHDA E, BILAN MARIA I, DMITRENOK ANDREY S, SHASHKOV ALEXANDER S, KUSAYKIN MIKHAIL I, STONIK VALENTIN A, NIFANTIEV: "Structure and biological activity of a fucosylated chondroitin sulfate from the sea cucumber Cucumaria japonica", GLYCOBIOLOGY, OXFORD UNIVERSITY PRESS, US, vol. 26, no. 5, 1 May 2016 (2016-05-01), US , pages 449 - 459, XP055920465, ISSN: 0959-6658, DOI: 10.1093/glycob/cwv119
WU JINJIN, XIA CONGHUA, GE WEIHONG: "Study on Anion Exchange Gum Purification of Radix Actinidia Chinensis Polysaccharides", ZHEJIANG ZHONGYIYAO DAXUE XUEBAO - JOURNAL OF ZHEIJIAN COLLEGE OF TCM, ZHEJIANG ZHONGYIYAO DAXUE, CN, vol. 34, no. 6, 30 November 2010 (2010-11-30), CN , pages 906 - 910, XP055920467, ISSN: 1005-5509, DOI: 10.16466/j.issn1005-5509.2010.06.032
Attorney, Agent or Firm:
LECOME INTELLECTUAL PROPERTY AGENT LTD. (CN)
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