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Patent Searching and Data


Title:
PREPARATION METHOD FOR DNA LIBRARY, AND ANALYSIS METHOD FOR DNA LIBRARY
Document Type and Number:
WIPO Patent Application WO/2020/259722
Kind Code:
A1
Abstract:
Provided is a preparation method for a DNA library, comprising a pre-library preparation process, the pre-library preparation process comprising DNA preparation, end repair and 3' A-tailing, linker connection using an anti-contamination linker, linker connected product purification, pre-library amplification, and amplified pre-library purification. Also provided are a use of the anti-contamination linker in preparing a test kit for DNA library capture, and a method for performing bioinformatic analysis on the DNA library prepared by means of the preparation method of the present invention. The preparation method of the present invention reduces the risk of cross-contamination between samples.

Inventors:
ZHANG ZHIHONG (CN)
WANG XIAOTIAN (CN)
ZHANG ZHEN (CN)
GU YUNZHAO (CN)
HAN YUSHENG (CN)
ZHANG HAIBO (CN)
Application Number:
PCT/CN2020/111054
Publication Date:
December 30, 2020
Filing Date:
August 25, 2020
Export Citation:
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Assignee:
GUANGZHOU BURNING ROCK DX CO LTD (CN)
International Classes:
C12Q1/6869; C40B50/06
Foreign References:
CN106835291A2017-06-13
CN107312776A2017-11-03
CN105861710A2016-08-17
CN110205683A2019-09-06
Attorney, Agent or Firm:
LIU, SHEN & ASSOCIATES (CN)
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