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Title:
PREPARATION METHOD FOR ENZYME-MODIFIED STEVIOSIDE, ENZYME FOR SAID PREPARATION METHOD, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/100676
Kind Code:
A1
Abstract:
A preparation method for enzyme-modified stevioside is provided in the invention and comprises: adding beta-fructofuranosidase into a solution having a stevioside raw material and sucrose dissolved therein so as to obtain a reaction liquid, adjusting the pH of said reaction liquid to 5.0-8.0, maintaining the reaction temperature at 20-45°C, and, after stirring and reacting, collecting the enzyme-modified stevioside, the stevioside raw material comprising one or more of stevioside and rebaudioside A, and the beta-fructofuranosidase being derived from microbacterium saccharophilum or aspergillus japonicus. The preparation method is highly effective and simple, low in cost, requires little time, has a high conversion rate, is environmentally friendly, and may be widely applied in industrial scale production. The enzyme for said preparation method and the application thereof are also provided in the present invention.

Inventors:
FU, Rongzhao (Room A-2, 1st Floor Building 1, The Third Sub-Park,Taohuayuan High-Tech Innovation Park, Tiegang, Xixiang Street, Baoan Distric, Shenzhen Guangdong 2, 518102, CN)
LIU, Lihui (Room A-2, 1st Floor Building 1, The Third Sub-Park,Taohuayuan High-Tech Innovation Park, Tiegang, Xixiang Street, Baoan Distric, Shenzhen Guangdong 2, 518102, CN)
JIANG, Ming (Room A-2, 1st Floor Building 1, The Third Sub-Park,Taohuayuan High-Tech Innovation Park, Tiegang, Xixiang Street, Baoan Distric, Shenzhen Guangdong 2, 518102, CN)
LIU, Yufeng (Room A-2, 1st Floor Building 1, The Third Sub-Park,Taohuayuan High-Tech Innovation Park, Tiegang, Xixiang Street, Baoan Distric, Shenzhen Guangdong 2, 518102, CN)
LIU, Wenshan (Room A-2, 1st Floor Building 1, The Third Sub-Park,Taohuayuan High-Tech Innovation Park, Tiegang, Xixiang Street, Baoan Distric, Shenzhen Guangdong 2, 518102, CN)
CHEN, Xiaochun (Room A-2, 1st Floor Building 1, The Third Sub-Park,Taohuayuan High-Tech Innovation Park, Tiegang, Xixiang Street, Baoan Distric, Shenzhen Guangdong 2, 518102, CN)
Application Number:
CN2018/088358
Publication Date:
May 31, 2019
Filing Date:
May 25, 2018
Export Citation:
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Assignee:
BONTAC BIO-ENGINEERING (SHENZHEN) CO., LTD. (Room A-2, 1st Floor Building 1, The Third Sub-Park,Taohuayuan High-Tech Innovation Park, Tiegang, Xixiang Street, Baoan Distric, Shenzhen Guangdong 2, 518102, CN)
JIANGXI BONTAC GREEN-BIOCATALYSIS ECOINDUSTRIAL PARK CO., LTD. (1-2 Floor, Building 28 Xingang Business Center,Sanghai Industrial Park of Nanchang Economic and,Technological Development Zon, Nanchang Jiangxi 5, 330115, CN)
International Classes:
C07H15/24; C07H15/256; C12N9/10; C12P19/18
Domestic Patent References:
WO2016144175A12016-09-15
WO2015171555A12015-11-12
Other References:
30 November 2008 (2008-11-30)
Attorney, Agent or Firm:
SCIHEAD IP LAW FIRM (Room 1508, Huihua Commercial & Trade BuildingNo. 80, Xianlie Zhong Road, Yuexi, Guangzhou Guangdong 0, 510070, CN)
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