Title:
PREPARATION METHOD FOR EXOGENOUS MOLECULE-LOADED CELLS, SUBSTRATE FOR PREPARATION OF EXOGENOUS MOLECULE-LOADED CELLS, AND EXOGENOUS MOLECULE-LOADED CELLS
Document Type and Number:
WIPO Patent Application WO/2017/097165
Kind Code:
A1
Abstract:
Exogenous molecule-loaded cells and a preparation method for exogenous molecule-loaded cells. The preparation method adopts a photoinduced perforating way for preparing, and comprises the following steps: (1) adopting a gold nano-structure to modify a cell culture substrate; (2) culturing cells in the cell culture substrate modified by the gold nano-structure; (3) adopting a laser source with a near-infrared band to irradiate the cell-loaded cell culture substrate; and (4) adding exogenous molecules into the cells. The preparation method can realize simple, direct, rapid and efficient cell delivery of exogenous molecules.
Inventors:
CHEN HONG (CN)
XU YAJUN (CN)
XUE HUI (CN)
XU YAJUN (CN)
XUE HUI (CN)
Application Number:
PCT/CN2016/108415
Publication Date:
June 15, 2017
Filing Date:
December 02, 2016
Export Citation:
Assignee:
SUZHOU FUNYA BIOTECH CO LTD (CN)
International Classes:
C12N15/87; C12N5/00; C12N13/00
Domestic Patent References:
| WO2014151888A1 | 2014-09-25 |
Foreign References:
| CN105420278A | 2016-03-23 | |||
| CN103649295A | 2014-03-19 | |||
| CN1653185A | 2005-08-10 | |||
| CN102776237A | 2012-11-14 | |||
| CN102703510A | 2012-10-03 |
Other References:
YAO, CUIPING ET AL.: "Effects of Selective Cell Targeting with Gold Nanoparticles by Laser Irradiation on Plasma Membrane Permeability", JOURNAL OF XI'AN JIAOTONG UNIVERSITY, vol. 42, no. 12, 31 December 2008 (2008-12-31), pages 1560 - 1564
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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