Title:
PREPARATION METHOD FOR MULTIPOTENT CELLS
Document Type and Number:
WIPO Patent Application WO/2013/069962
Kind Code:
A1
Abstract:
The present invention relates to a chemical preparation method for multipotent cells derived from skeletal muscle and a use thereof, and provides a chemical preparation method for multipotent cells derived from
differentiated mammal cells, comprising the following steps: (a) obtaining differentiated cells from a mammal; and (b) treating the differentiated cells with a compound for inducing the downregulation of p21 to obtain multipotent cells. It is possible to provide myogenic lineage, adipogenic lineage, osteogenic lineage or neurogenic lineage using the preparation method for multipotent or totipotent cells of the present invention. A chemical-based approach is important for developing strategies for inducing limb regeneration in a mammal, and a novel approach to develop multipotent or totipotent cells from terminally differentiated cells is suggested.
Inventors:
WILLIAMS DARREN (KR)
JUNG DA-WOON (KR)
KIM WOONG-HEE (KR)
JUNG DA-WOON (KR)
KIM WOONG-HEE (KR)
Application Number:
PCT/KR2012/009338
Publication Date:
May 16, 2013
Filing Date:
November 07, 2012
Export Citation:
Assignee:
KWANGJU INST SCI & TECH (KR)
International Classes:
C12N5/077; C12N1/38; C12N5/071
Other References:
JUNG, D-W. ET AL.: "Novel Chemically Defined Approach To Produce Multipotent Cells from Terminally Differentiated Tissue Syncytia", ACS CHEMICAL BIOLOGY, vol. 6, 15 February 2011 (2011-02-15), pages 553 - 562
ZHANG, Y. ET AL.: "Dedifferentiation and Proliferation of Mammalian Cardiomyocytes", PLOS ONE, vol. 5, no. 9, 3 September 2010 (2010-09-03), pages 1 - 13, XP055067808
SHAN, SW. ET AL.: "Induction of growth arrest and polycomb gene expression by reversine allows C2C12 cells to be reprogrammed to various differentiated cell types", PROTEOMICS, vol. 7, no. 23, 23 December 2007 (2007-12-23), pages 4303 - 4316, XP055067565
KIM, W-H. ET AL.: "Small Molecules That Recapitulate the Early Steps ofUrodele Amphibian Limb Regeneration and Confer Multipotency", ACS CHEMICAL BIOLOGY, vol. 7, 23 January 2012 (2012-01-23), pages 732 - 743
ZHANG, Y. ET AL.: "Dedifferentiation and Proliferation of Mammalian Cardiomyocytes", PLOS ONE, vol. 5, no. 9, 3 September 2010 (2010-09-03), pages 1 - 13, XP055067808
SHAN, SW. ET AL.: "Induction of growth arrest and polycomb gene expression by reversine allows C2C12 cells to be reprogrammed to various differentiated cell types", PROTEOMICS, vol. 7, no. 23, 23 December 2007 (2007-12-23), pages 4303 - 4316, XP055067565
KIM, W-H. ET AL.: "Small Molecules That Recapitulate the Early Steps ofUrodele Amphibian Limb Regeneration and Confer Multipotency", ACS CHEMICAL BIOLOGY, vol. 7, 23 January 2012 (2012-01-23), pages 732 - 743
Attorney, Agent or Firm:
YANG, Boo-Hyun (1659-2 Inhundong Gwanak-gu, Seoul 151-832, KR)
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Claims: