Title:
PREPARATION METHOD FOR NANO MAGNETIC BEAD, FREE DNA EXTRACTION KIT AND EXTRACTION METHOD
Document Type and Number:
WIPO Patent Application WO/2020/200201
Kind Code:
A1
Abstract:
Disclosed by the present application are a magnetic bead for extracting free DNA and a preparation method therefor, on the basis of which, further disclosed is a free DNA extraction kit based on the magnetic bead, comprising sample lysate, binding solution, primary washing solution, secondary washing solution and eluent. The sample lysate comprises nuclease-free water, reagent A and reagent B. The reagent A is at least one of guanidine isothiocyanate, guanidine hydrochloride, sodium iodide, lithium acetate, lithium perchlorate and sodium perchlorate. The reagent B is at least one of sodium dodecyl benzene sulfonate, fatty acid glyceride, Tween20, Triton-X100, SDS, NP40 and polyoxyethylene nonionic surfactant. In addition to the sample lysate, the binding solution comprises reagent C, and the reagent C is at least one of isopropanol, butanol, methanol and absolute ethanol. The primary washing solution is the same as the binding solution in terms of components. The secondary washing solution is one of nuclease-free water, sodium chloride solution with a molar concentration of 0.1-1M, Tris buffer or TE buffer. The eluent is one of nuclease-free water, Tris buffer or TE buffer. Also disclosed by the present application is a method for extracting the free DNA. The present application may greatly improve the extraction efficiency and purity of free DNA.
Inventors:
WAN HAO (CN)
ZHANG BO (CN)
ZHAO SHUTING (CN)
GE DONGLIANG (CN)
ZHANG FEI (CN)
ZHANG HAIYAN (CN)
ZHANG BO (CN)
ZHAO SHUTING (CN)
GE DONGLIANG (CN)
ZHANG FEI (CN)
ZHANG HAIYAN (CN)
Application Number:
PCT/CN2020/082451
Publication Date:
October 08, 2020
Filing Date:
March 31, 2020
Export Citation:
Assignee:
SHENZHEN APOSTLE SUSTECH LTD (CN)
International Classes:
B01J20/26; B01D15/08; B01J20/28; B01J20/30; C12N15/10
Foreign References:
| CN110038533A | 2019-07-23 | |||
| CN109913447A | 2019-06-21 | |||
| CN105349532A | 2016-02-24 |
Other References:
JIANG JING: "A facile method to the Ni0.8Co0.2Fe2O4 nanocrystalline via a refluxing route in ethylene glycol", MATERIALS LETTERS, vol. (61), no. 14-15, 27 November 2006 (2006-11-27), XP022066807, ISSN: 0167-577X, DOI: 20200519095351X
JIANG JING: "A facile method to the Ni0.8Co0.2Fe2O4 nanocrystalline via a refluxing route in ethylene glycol", MATERIALS LETTERS, vol. (61), no. 14-15, 27 November 2006 (2006-11-27), XP022066807, ISSN: 0167-577X, DOI: 20200519095351X
LIU, HONG ET AL.: "Studies on catalytic oxidation of VB1 by PEG modified Fe3O4 nanoparticles", JOURNAL OF TIANJIN AGRICULTURAL UNIVERSITY, vol. 25, no. 4,, 31 December 2018 (2018-12-31), ISSN: 1008-5394, DOI: 20200519102055Y
JIANG JING: "A facile method to the Ni0.8Co0.2Fe2O4 nanocrystalline via a refluxing route in ethylene glycol", MATERIALS LETTERS, vol. (61), no. 14-15, 27 November 2006 (2006-11-27), XP022066807, ISSN: 0167-577X, DOI: 20200519095351X
LIU, HONG ET AL.: "Studies on catalytic oxidation of VB1 by PEG modified Fe3O4 nanoparticles", JOURNAL OF TIANJIN AGRICULTURAL UNIVERSITY, vol. 25, no. 4,, 31 December 2018 (2018-12-31), ISSN: 1008-5394, DOI: 20200519102055Y
Attorney, Agent or Firm:
SHENZHEN HUAQIN INTELLECTUAL PROPERTY AGENCY FIRM (GENERAL PARTNERSHIP) (CN)
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