Title:
PREPARATION METHOD FOR AND USES OF ANTIFREEZE PROTEIN OF SOYBEAN
Document Type and Number:
WIPO Patent Application WO/2017/219952
Kind Code:
A1
Abstract:
The present invention provides a preparation method for an antifreeze protein of soybean. The method comprises the steps of: transforming plasmids containing the expression sequence of the antifreeze protein to expressed escherichia coli for protein expression; purifying the protein by using an affinity chromatography method; and excising an His label by using thrombin after purification, so as to obtain the antifreeze protein. The antifreeze protein is a PM1 protein or a PM1-N oligopeptides. The present invention also provides uses of the PM1 protein or the PM1-N oligopeptides in the preparation of transgenetic antifreeze or cold-resistant plant products and in the preparation of cryopreserved protecting agents for biological products.
Inventors:
ZHENG YIZHI (CN)
LIU GUOBAO (CN)
CHEN LIYI (CN)
PENG XIANMING (CN)
LIU GUOBAO (CN)
CHEN LIYI (CN)
PENG XIANMING (CN)
Application Number:
PCT/CN2017/089103
Publication Date:
December 28, 2017
Filing Date:
June 20, 2017
Export Citation:
Assignee:
UNIV SHENZHEN (CN)
International Classes:
C07K1/22; C12N15/70; C12P21/04
Foreign References:
| CN103415533A | 2013-11-27 | |||
| CN105969788A | 2016-09-28 | |||
| CN102010869A | 2011-04-13 |
Other References:
GAO YANG: "Expression of soybean GmPM1 and GmPM9 proteins enhances resistance to copper stress in plant", CHINA MASTER'S THESES, no. 12, 31 December 2015 (2015-12-31), pages 39 - 41
SHIH M: "Characterization of two soybean (Glycine max L.) LEA IV pro- teins by circular dichroism and fourier transform infrared spectrometry", PLANT AND CELL PHYSIOLOLGY, vol. 51, no. 3, March 2010 (2010-03-01) - 31 December 2010 (2010-12-31), pages 395 - 407, XP055601941, ISSN: 0032-0781, DOI: 10.1093/pcp/pcq005
CHEN LIYI ET AL.: "Protection effect of soybean PM1 protein on liposome and the rabbit red blood cells", JOURNAL OF SHENZHEN UNIVERSITY SCIENCE & ENGINEERING, vol. 5, no. 33, 30 September 2016 (2016-09-30)
SHIH M: "Characterization of two soybean (Glycine max L.) LEA IV pro- teins by circular dichroism and fourier transform infrared spectrometry", PLANT AND CELL PHYSIOLOLGY, vol. 51, no. 3, March 2010 (2010-03-01) - 31 December 2010 (2010-12-31), pages 395 - 407, XP055601941, ISSN: 0032-0781, DOI: 10.1093/pcp/pcq005
CHEN LIYI ET AL.: "Protection effect of soybean PM1 protein on liposome and the rabbit red blood cells", JOURNAL OF SHENZHEN UNIVERSITY SCIENCE & ENGINEERING, vol. 5, no. 33, 30 September 2016 (2016-09-30)
Attorney, Agent or Firm:
HENSEN INTELLECTUAL PROPERTY FIRM (CN)
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