Title:
PREPARATION AND USE OF HIGH-ACTIVITY AND HIGH-SAFETY CARDAMINE VIOLIFOLIA SELENOPROTEIN STANDARD SAMPLE
Document Type and Number:
WIPO Patent Application WO/2024/032812
Kind Code:
A1
Abstract:
Disclosed are preparation and use of a high-activity and high-safety cardamine violifolia selenoprotein standard sample. The preparation method comprises: hydrolyzing cardamine violifolia powder with cellulase, carrying out enzymolysis, and centrifuging to obtain a supernatant; and adding a complex enzyme into the supernatant for continuous enzymolysis treatment and synchronously carrying out electrodialysis, subjecting the resulting reaction solution to enzyme deactivation, concentrating, and drying to obtain the selenoprotein standard sample. The complex enzyme consists of alkaline protease, trypsin, papain, protease K, and protease XIV. The cardamine violifolia selenoprotein standard sample prepared according to the present invention has the advantages of extremely low heavy metal content, less ash content, high protein content, high total selenium content, an organic selenium proportion of up to 99.9% or more, high biological activity, high safety, and the like, wherein the organic selenium is mainly in the form of selenocysteine/selenocystine and accounts for 90% or more of the total selenium content. Therefore, the lack of a selenoprotein standard sample on the market is expected to be solved.
Inventors:
LIU HAIYUAN (CN)
CONG XIN (CN)
XU BO (CN)
ZHANG YUE (CN)
LI JIE (CN)
LI CUI (CN)
ZHU ZHENZHOU (CN)
CHENG SHUIYUAN (CN)
CONG XIN (CN)
XU BO (CN)
ZHANG YUE (CN)
LI JIE (CN)
LI CUI (CN)
ZHU ZHENZHOU (CN)
CHENG SHUIYUAN (CN)
Application Number:
PCT/CN2023/114545
Publication Date:
February 15, 2024
Filing Date:
August 23, 2023
Export Citation:
Assignee:
ENSHI SE RUN MATERIAL ENGINEERING TECH CO LTD (CN)
International Classes:
C12P21/06; A23J1/00; A23J3/14; C07K1/14; C07K1/34
Foreign References:
| CN116042756A | 2023-05-02 | |||
| CN112515032A | 2021-03-19 | |||
| CN105795095A | 2016-07-27 | |||
| CN112535233A | 2021-03-23 | |||
| CN110256600A | 2019-09-20 | |||
| US4486282A | 1984-12-04 | |||
| CN106916871A | 2017-07-04 | |||
| CN107815483A | 2018-03-20 |
Attorney, Agent or Firm:
SOON IP LAW FIRM (CN)
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