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Title:
PRETREATMENT METHOD, PRETREATMENT SOLUTION, KIT FOR VIRUS NUCLEIC ACID DETECTION, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/174673
Kind Code:
A1
Abstract:
The present invention belongs to the field of virus nucleic acid detection. Specifically, the present invention provides a pretreatment method for virus nucleic acid detection. The method comprises mixing a pretreatment solution containing a sample with a nucleic acid releasing agent and a qPCR amplification reagent. The pretreatment solution comprises Tris-HCl, EDTA-2Na, sodium chloride, a ribonuclease inhibitor and an antibiotic; and the pretreatment solution has a pH of 6.5-8.0.

Inventors:
DAI LIZHONG (CN)
JI BOZHI (CN)
DENG ZHONGPING (CN)
LIU JIA (CN)
TAN DEYONG (CN)
FAN XU (CN)
Application Number:
PCT/CN2020/090052
Publication Date:
September 10, 2021
Filing Date:
May 13, 2020
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Assignee:
SANSURE BIOTECH INC (CN)
International Classes:
C12Q1/6806; C12Q1/6851; C12Q1/70; C12R1/93
Domestic Patent References:
WO2005042030A12005-05-12
WO2001001129A22001-01-04
WO2009153299A12009-12-23
Foreign References:
CN110982876A2020-04-10
CN101970660A2011-02-09
AU2006214059A12006-08-24
CN103184297A2013-07-03
CN105392363A2016-03-09
CN1940087A2007-04-04
Other References:
CROMEANS THERESA; JOTHIKUMAR NARAYANAN; LEE JEONGSU; COLLINS NIKAIL; BURNS CARA C.; HILL VINCENT R.; VINJÉ JAN: "A new solid matrix for preservation of viral nucleic acid from clinical specimens at ambient temperature", JOURNAL OF VIROLOGICAL METHODS, ELSEVIER BV, NL, vol. 274, 11 September 2019 (2019-09-11), NL, XP085893955, ISSN: 0166-0934, DOI: 10.1016/j.jviromet.2019.113732
See also references of EP 4006169A4
Attorney, Agent or Firm:
ADVANCE CHINA IP LAW OFFICE (CN)
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