Title:
PRIMER SET FOR IDENTIFYING BACTERIAL SPECIES IN PROBIOTICS COMPOSITION AND METHOD FOR IDENTIFYING BACTERIAL SPECIES USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/234577
Kind Code:
A1
Abstract:
The present invention relates to a primer set for identifying bacterial species in a probiotics composition and a method for identifying bacterial species using same. Particularly, by constructing a set of primers targeting an intergenic transcribed spacer (ITS) region between 16S rRNA and 23S rRNA of a probiotic species and a pathogenic bacterial species, and performing metagenomic analysis of the probiotics composition by using same, it was confirmed that 21 species of probiotics and 4 species of pathogenic bacteria can be identified with high resolution and accuracy, and thus, the primer set can be effectively used to identify species in a probiotic composition.
Inventors:
KIM MYUNG-SOO (KR)
KANG EUN YOUNG (KR)
LIM JONG-HYUN (KR)
KIM BYUNG-YONG (KR)
KANG EUN YOUNG (KR)
LIM JONG-HYUN (KR)
KIM BYUNG-YONG (KR)
Application Number:
PCT/KR2023/006187
Publication Date:
December 07, 2023
Filing Date:
May 08, 2023
Export Citation:
Assignee:
CHONGKUNDANG HEALTHCARE CORP (KR)
International Classes:
C12Q1/689; C12Q1/686
Foreign References:
| KR20220010100A | 2022-01-25 | |||
| KR20040077544A | 2004-09-04 | |||
| KR101695059B1 | 2017-01-23 | |||
| KR102334331B1 | 2021-12-02 | |||
| JPH11123093A | 1999-05-11 | |||
| KR20200029689A | 2020-03-19 | |||
| KR20200027900A | 2020-03-13 |
Other References:
SEONG YOUNG-JEPARK MYUNG-SOO: "Current status of domestic and international probiotic product development", FOOD SCIENCE AND INDUSTRY, vol. 52, no. 3, pages 229 - 240
LEE JOO-HOON: "Study on probiotic genetic characteristics and safety confirmation method", November 2011, MINISTRY OF FOOD AND DRUG SAFETY
MASSIMILIANO ET AL., APPL. ENVIRON. MICROBIOL., vol. 70, 2004, pages 6147 - 6156
LEE JOO-HOON: "Study on probiotic genetic characteristics and safety confirmation method", November 2011, MINISTRY OF FOOD AND DRUG SAFETY
MASSIMILIANO ET AL., APPL. ENVIRON. MICROBIOL., vol. 70, 2004, pages 6147 - 6156
Attorney, Agent or Firm:
KIM, Bo Jung (KR)
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