This invention relates to pro-healthy food product, which enables fortification of diverse human diet with pro-healthy ingredients.

The disclosure CN 1108891 describes milk product containing the source of omega- 3 fatty acids and its consumption simultaneously prevents and treats sclerosis, femoral artery embolism and heart attack.

Patent application CN 102090452A discloses milk product, containing phosphatidylserine and polyunsaturated omega- 3 fatty acids, which supports cardiovascular system and memory. Oil is added to milk in form of an emulsion.

International patent application WO 2012129222 Al describes the methods for improving bone health in infants using LC-PUFA in liquid or powder form (obtained by spray-drying) consumed e.g. in nutritional bars, powders reconstituted in water or in liquid form in drinks (e.g. emulsions).

Description polish patent application P.391985 reveals a preparation facilitating the recovery of proper microbiological equ librium as well as regulating the pH of intestinal contents, with the concurrent nourishment of intestinal mucosal cells, which essentially ensures or promotes the proper functioning of the distal portions of the gastrointestinal tract, maintains the microbiological and acid base equilibrium of the gastrointestinal tract, characterized by the fact that it comprises live bacteria: Lactobacillus rhamnosus GG, Bifidobacterium and Streptococcus thermophilus, as well as fumaric acid, citric acid, malic acid, and sorbic acid in a triglyceride matrix. The preparation is in the form of cellulose capsules in which the bacteria and acids are enclosed in a triglyceride matrix. International patent application WO 2013001089 reveals dry composition containing lactic acid bacteria. There are known from US patent application US 20120039956 the compositions and drying methods for preserving sensitive bioactive materials, such as peptides, proteins, hormones, drugs, yeast or bacteria, in storage. The composition is prepared by dispersing all the solid components in a solution and then snap-frozen to form small beads, strings or droplets and freezing them under a vacuum pressure. The description of patent application EP2532354 (Al ) discloses spray-dried Lactobacillus cells for treating and preventing Helicobacter pylori infections in humans or animals and the production of a composition comprising mixing spray-dried Lactobacillus cells with a carrier. According to the description the carrier substances include starch, maltodextrin, cellulose, levulose, lactose, dextrose, and mixtures of such substances. The invention disclosed in US6010725A description concerning spray-drying process of microorganisms, including lactic acid bacteria, in a spray-drying device having a heated air inlet temperature of 200-400 adjusted so as to obtain at least 1% survival of the microorganisms after drying in the product. The invention relates to the drying of mixture of microorganisms suspended in water. The invention US6827953 B l provides a composition comprising at least three lactic acid bacteria selected from the group consisting of Lactobacillus delbrueckii, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus fertnentum, Lactobacillus casei, Lactobacillus rhamnosus, Lactococcus lactis and Streptococcus thermophilus, mixed with yeast Saccharomyces cerevisiae, intended as a functional food additive in spray- dried or lyophilized form, without using additional carrier substances or oils. Patent application US20100074994 A! discloses a product containing live probiotic microorganisms designed to be used in food products and foodstuffs and is obtained by vacuum drying of probiotic microorganisms cells in a carrier composed by short - chained sugars, proteins or polysaccharides. The number of probiotic microorganisms ranges from 10 ^J to 10 ¹⁴ live cells in a gram of dry product.

At retail there are supplements containing n-3 fatty acids and also probiotic preparations containing probiotic microorganisms. Surprisingly it turned out that it is possible to produce a pro-healthy product in powdered form which consists of microcapsules with immobilized unsaturated fatty acids and probiotic microorganisms.

According to the present invention the pro-healthy food product, which is composed of microcapsules with immobilized probiotic microorganisms, is characterised in that it has the form of a powder containing 22.3 - 60 weight % of the source of essential unsaturated fatty acids (EFA) and 10 ⁶ - 10 ⁱ³ live cells of probiotic microorganisms in a gram of the product. Essential fatty acids (EFA) are exogenous fatty acids which cannot be synthesised in human organism and have to be supplied with food. The envelope of the capsule is formed of a natural polymer or modified natural polymer at the amount of 10-50 weight % and of maltodextrin or mixture of maltodextrins with various values of Dextrose Equivalent (DE) and/or glucose syrup at the amount of 3-60 weight %. Glucose syrup can be used instead of maltodextrin. The envelope accounts for 30-63 weight % of the microcapsule. The concentration of envelope components in microcapsules can be higher than 63 weight % at the expense of the amount of other components; however said range of envelope components concentration is optimal to obtain proper parameters of the immobilisate. It is possible to use only one type of maltodextrin together with modified starch or even use only one type of maltodextrin as a carrier. Maltodextrins with different values of Dextrose Equivalent are used, but the optimal range of DE is from 1 1 to 20.

According to the present invention the product also contains up to 5 weight % of emulsifier from the group of fatty acids and sorbitol esters and/or emulsifier from the group of oxyethynelated fatty acids and sorbitol esters. Higher or lower emulsifier concentrations can be used with slight change in the quality of emulsion and resulting immobilisate. The use of higher concentration is not recommended due to the fact that used emulsifiers, even though they are food additives, are not natural substances and ALARA (as low as reasonably acceptable) approach is suggested. Moreover, in described product other emulsifiers permitted for food use can be applied. However, their use most often involves the introduction of additional individual operations during production of pro-healthy food product, which may affect the quality of unsaturated fatty acids. The inventors adopted the rule that the simplest and shortest technological process the better. Additionally, one trial was performed using an emulsifier not approved for use in foods. The result was acceptable, even though the product obtained was obviously not approved for consumption.

According to the present invention the sources of essential unsaturated fatty acids (EFA) are: cod liver oil (containing the esters of 1 ,2,3-propanetriol and/or long-chained polyunsaturated fatty acids, including docosahexaenoid and eicosapentaenoic acids), and/or evening primrose oil and/or borage seed oil and/or blackcurrant seed oil. Also synthetic source of n-3 fatty acids can be used in form of e.g. ethyl esters of fatty acids, especially docosahexaenoid and eicosapentaenoic fatty acids.

According to the present invention the pro -healthy preparation contains probiotic microorganisms - microorganisms (bacteria or yeasts) with at least initially proved probiotic features according to the definition of F AO/WHO, showing their pro-healthy effect on human organism. Preferably as probiotic microorganism the following genera of bacteria are used: Bacillus and/or Bifidobacterium and/or Enterococcus and/or Lactobacillus and/or Lactococcus and/or Leuconostoc and/or Pediococcus and/or Propionihacterium and/or Streptococcus and/or yeasts from the genus Saccharomyces. Preferably the mixture of maltodextrins contains 20-80 weight % of maltodextrin with high value of DE and 20-80 weight % of maltodextrin with low value of DE.

The envelope - polymer carrier, protects the immobilisate (probiotic microorganisms and unsaturated fatty acids) against the adverse effect of outer environment, especially oxygen in the air. The envelope can be formed from any polymer approved for food use. Preferably that polymer is modified starch. Instead of modified starch a modified cellulose can be used (e.g. methylcellulose or hydroxy-propyl methylcellulose) and also gum arabic, pectins (e.g. beetroot) or whey proteins or milk proteins concentrate. These components can be used individually or in various configurations. It is possible to increase the amount of modified starch itself or use it as a single component but the resulting product will be characterised by lower quality. Preferably modified starch is the sodium salt of starch octenylsuccinate.

According to the present invention the product also contains antioxidants at the amount of 0.02-2.5 weight %. Antioxidants are added in order to increase the shelf life of obtained powder and to give it taste and smell desired for particular use.

Preferably the antioxidants used are rosemary extract or oregano oil with rosemary extract at weight ratio of 0.3 - 1.25, or rosemary extract with oil of cloves and oregano oil a weigh ratio from 1 :1:1 to 1: 1:2. The addition of antioxidants affects the smell of the powder, and thus the notes 'sweet' and 'tomato' are characteristic for single component variant, 'herbal' - for two-component variant, and 'sweet' and 'fresh' for three-component variant. With respect to taste this involves the notes of 'tomato' and 'caramel' for single component variant, 'herbal', 'sweet' and 'sour' for two-component variant, and 'herbal', 'sour' and 'tomato' for three-component variant. These are the natural antioxidants, but the pro-healthy food product can also contain artificial ones. The powder may not contain them at all or contain more than said 2.5 weight % (with respect to EFA). In the first case, however, the quality of encapsulated unsaturated fatty acids deteriorates rapidly within 3 months. In the second case certain taste and/or aroma notes become predominant, what reduces the versatility of the immobilisate as a food additive to a wide range of food products, since it changes the taste and smell profile of fortified product. Performed studies showed that the increase in antioxidant concentration above the said values does not significantly affect the stabilization of unsaturated fatty acids and sometimes leads to slight reduction in their quality.

According to the present invention the product, besides antioxidants, can also contain up to 3.2 weight % of rapeseed (canola) or soy lecithin and/or up to 3.1 weight % hydrophobic natural colour additives. The immobilisate can also contain up to 3.5 weight % of natural hydrophilic colour additives, anti-caking agents approved for food use and/or water soluble vitamins. These substances improve the quality, among others processing or organoleptic quality of obtained powder.

The advantage of the present invention is that it contains both unsaturated fatty acids essential for human organism (EFA) and probiotic microorganisms, and simultaneously has a form of dry powder, which facilitates its storage and application. The powder is composed of microcapsules with polymer capsule/envelope, containing immobilized probiotic microorganisms. Powder form allows easy dosage and application. The advantage of the present invention is based on the fact that, in contradiction to known products, it does not have an emulsion but a powder form, which gives it higher stability and lower susceptibility to microbial changes as well, as low water activity. Simultaneously the storage and transport of the powder is easier than in case of emulsion (where water can constitute up to 75% of emulsion weight).

The powder obtained according to the present invention is an additive to ready- to-eat products and meals (used e.g. at home as a sprinkle) or component of ready food products enhancing their nutritive value. The additive can be applied similarly to seasoning in traditional kitchen or used on an industrial scale as an additive fortifying the composition of food products. Obtained food additive, being composed of unsaturated fatty acids (EFA) and probiotic microorganisms, has complex effect on cardio-vascular system and digestive tract, and additionally can be used in various food products or consumed in its primary form of microcapsules. Obtained product can be applied as a sprinkle on fresh salads both at home and in mass catering operations, restaurant, bars etc. Immobilisate is well suited as a supplement in ready-to-cook products such as dumplings. The powder can be used as a functional supplement in food industry, among others, to fortify the products of chocolate, baking and meat industry. According to the present invention the product can be stored in MAP (modified atmosphere) using such gases as nitrogen and/or carbon dioxide. It can also be stored in cold temperatures.

The invention is presented in the following examples.

Example I

The following components are added into 15.98 kg of water: 0.67 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.17 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue and 3.19 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short mixing ( 10 min) of the source of n-3 fatty acids from cod liver oil at the amount of 1.89 kg with 140.4 g of sorbitan monooleate and 60.18 g of polyoxyethyienesorbitan monooleate and with 9.48 g of rosemary extract. Next, 2.08 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion. Simultaneously, microbial biomass of probiotic bacteria is obtained - from the pure culture of Lactobacillus rhamnosus at late logarithmic phase of growth by centrifugation at 4000 rpm. Live probiotic bacteria in form of centrifuged biomass of bacterial cells of Lactobacillus rhamnosus are added to the emulsion at the temperature of 27 °C at the amount of 4.57 x 10 ^l CPU (Colony Forming Units) per miililiire of the emulsion. The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 75°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic bacteria Lactobacillus rhamnosus GG - their total number in final product being 4.88 x 10 ¹⁰ CFU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 63 weight %, polymer concentration - 50 weight %, maltodextrin and/or glucose syrup concentration - 13 weight %, concentration of the source of n-3 fatty acids - 30.67 weight %, emulsifiers concentration - 3.26 weight %, antioxidants concentration - 0.15 weight %.

Example II

The following components are added into 15.10 kg of water: 2.83 kg of maitodextrin with DE of 19.9 and 95.0% dry mass residue, 0.71 kg of maltodextrin with DE of 1 1.9 and 95.0% dry mass residue, and 1.37 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 7.91 kg with 98.88 g of sorbitan monooleate and 42.38 g of polyoxyethylenesorbitan monooleate, and also with 9.48 g of rosemary extract. Next, 8.01 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.3. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion. Simultaneously, microbial bio mass of probiotic bacteria is obtained - from the pure culture of Lactobacillus rhamno s GG at late logarithmic phase of growth by centrifugation at 4000 rpm. Live probiotic bacteria in form of centrifuged biomass of bacterial cells of Lactobacillus rhamnosus GG are added to the emulsion at the temperature of 27°C at the amount of 4.57 x 10 ¹² CFU (Colony Forming Units) per millilitre of the emulsion. The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 75°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic bacteria Lactobacillus rhamnosus GG - their total number in final product reaching 4.88 x 10 ¹ CFU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 35.72 weight %, polymer concentration - 10 weight %, maitodextrin and/or glucose syrup concentration - 25.72 weight %, concentration of the source of n-3 fatty acids - 60 weight %, emulsifiers concentration - 1.07 weight %, antioxidants concentration - 0.3 weight %.

Example III

The following components are added into 16.05 kg of water: 2.22 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.63 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 1.10 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 7.51 kg with 177.98 g of sorbitan monooieate and 76.28 g of polyoxyethylenesorbitan monooleate, and also with 37.76 g of rosemary extract and 395.49 g of nettle extract. Next, 8.4 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre- emulsion with volumetric ratio of dispersed phase of 0.3. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion. Simultaneously, microbial biomass of probiotic bacteria is obtained - from the pure culture of Lactobacillus rhamnosus GG at late logarithmic phase of growth by centrifugation at 4000 rpm. Live probiotic bacteria in form of centrifuged biomass of bacterial cells of Lactobacillus rhamnosus GG are added to the emulsion at the temperature of 27°C at the amount of 4.57 x 10 ¹² CPU (Colony Forming Units) per miliilitre of the emulsion. The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 75°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic bacteria Lactobacillus rhamnosus GG - their total number in final product reaching 4.88 x 10 ^l0 CPU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 30 weight %, polymer concentration - 8.4 weight %, maltodextrin and/or glucose syrup concentration - 21.60 weight %;, concentration of the source of n-3 fatty acids - 59.42 weight %, emulsifiers concentration - 2.01 weight %, lecithin concentration - 2.23 weight %. antioxidants concentration - 0.3 weight %, colour additives concentration - 3.13 weight %.

Example IV

The following components are added into 14.35 kg of water: 3.87 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.97 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 0.80 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 1 .89 kg with 140.41 g of sorbitan monooieate and 60.18 g of polyoxyethylenesorbitan monooleate, and also with 9.48 g of rosemary extract. Next, 2.08 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion. Simultaneously, microbial bio mass of pro bio tic bacteria is obtained - from the pure culture of Lactobacillus rhamnosus at late logarithmic phase of growth by centrifugation at 4000 rpm. Live probiotic bacteria in form of centrifuged biomass of bacterial cells of Lactobacillus rhamnosus are added to the emulsion at the temperature of 27°C at the amount of 4.57 x 10 ^lz CPU (Colony Forming Units) per millilitre of the emulsion. The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 75°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic bacteria Lactobacillus rhamnosus GG - their total number in final product reaching 4.88 x 10 ^{i 0} CPU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 70 weight %, polymer concentration - 10 weight %, maltodextrin and/or glucose syrup concentration - 60 weight %, concentration of the source of n-3 fatty acids - 24.37 weight %, eniuisifiers concentration - 2.59 weight %, antioxidants concentration - 0.12 weight %.

Example V

The following components are added into 15.55 kg of water: 2.88 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.81 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 0.81 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 1 .53 kg with 234.02 g of sorbitan monooieate and 100.29 g of poiyoxyethylenesorbitan monooleate, and also with 80.53 g of rosemary extract, 222.87 g of lecithin and 171.72 g of paprika extract. Next, 2.08 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre- emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion. Simultaneously, microbial biomass of probiotic microorganisms is obtained - the mixture of probiotic bacteria Lactobacillus casei. Streptococcus thermophilus and Bifidobacterium bifidum,, and probiotic yeasts Saccharomyces boulardii— from pure microbial cultures at late logarithmic phase of growth by centrifugation at 4000 rpm. The mixture of live probiotic microorganisms: Lactobacillus casei, Streptococcus thermophilus. Bifidobacterium bifidum and Saccharomyces boulardii is introduced to the emulsion at the amount of, respectively, 4.33 x 10 ^¾0 CFU (Colony Forming Units), 7.61 x 10 ⁹ CFU, 4.54 x 10 ⁹ CFU and 6.68 x 10 ⁹ CFU per millilitre of the emulsion. The components are gently stirred obtaining homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 76°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic microorganisms - the mixture of probiotic bacteria Lactobacillus casei, Streptococcus thermophilus and Bifidobacterium bifidum, and probiotic yeasts Saccharomyces boulardii- their total number in final product is 7 25 x 10 ^s CFU per g of powder. The product is added as a sprinkle to a salad of rugola, roasted sunflower seeds and feta cheese at the amount of Ig of powder per each lOOg of salad.

The parameters of obtained powder are as follows: total concentration of capsule components - 62.97 weight %, polymer concentration - 11.34 weight %, maltodextrin and/or glucose syrup concentration - 51.64 weight %, concentration of the source of n-3 fatty acids - 22.31 weight %, emulsifiers concentration - 4.87 weight %, lecithin concentration - 3.25 weight %, antioxidants concentration - 1.17 weight %, concentration of colour additives - 2.5 weight %.

Example VI

The following components are added into 16.02 kg of water: 0.15 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.04 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 3.79 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 1.89 kg with 140.14 g of sorbitan monooleate and 100.29 g of polyoxyethylenesorbitan monooieate, and also with 80.53 g of rosemary extract. Next, 2.07 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion. Simultaneously, microbial biomass of probiotic microorganisms is obtained - the mixture of pro bio tic bacteria Enterococcus faecium and Lactococcus lactis - from mixed microbial culture at late logarithmic phase of growth by filtration through filter with mesh size of 0.22 micrometers. The mixture of live probiotic microorganisms: Enterococcus faecium and Lactococcus lactis is introduced to the emulsion at the total amount of 6.33 x 10 ^W CFU (Colony Forming Units) per millilitre of the emulsion. The components are gently- stirred obtaining homogenous emulsion. Next, the emulsion is spray-dried using spray- drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 80°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic microorganisms - the mixture of probiotic bacteria Enterococcus faecium and Lactococcus lactis- their total number in final product being 1.33 x 10 ⁹ CFU per g of powder. The product is added as a sprinkle to a salad of ruguia, roasted sunflower seeds and feta cheese at the amount of lg of powder per each lOOg of salad.

The parameters of obtained powder are as follows: total concentration of capsule components - 62.88 weight %, polymer concentration - 59.88 weight %, maltodextrin and/or glucose syrup concentration - 3 weight %, concentration of the source of n-3 fatty acids - 30.91 weight %, emulsifiers concentration - 3.29 weight %, antioxidants concentration - 0.02 weight %.

Example VII

The following components are added into 16.02 kg of water: 0.14 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.38 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 3.79 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 2.95 kg with 147.15 g of sorbitan monooieate and 63.07 g of polyoxyethylenesorbitan monooleate, and also with 1.47 g of rosemary extract. Next, 3.13 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.14. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenizaiion, to receive primary emulsion. Simultaneously, microbial biomass of probiotic bacteria is obtained - from the pure culture of Lactobacillus rhamnosus at late logarithmic phase of growth by centrifugation at 5000 rpm. Thus obtained live probiotic bacteria Lactobacillus rhamnosus are added to the emulsion at the temperature of 30°C at the amount of 4.25 x 10 CFU (Colony Forming Units) per millilitre of the emulsion. The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 77°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic bacteria Lactobacillus rhamnosus - their total number in final product being 1.63 x 10 ^1J CFU per g of powder. Tliis product is added to cold- mixed chocolate-based product at the amount of 0.25kg per 25kg of weight.

The parameters of obtained powder are as follows: total concentration of capsule components - 53.25 weight %, polymer concentration - 30 weight %, maltodextrin and/or glucose syrup concentration - 23.25 weight %, concentration of the source of n-3 fatty acids - 40.90 weight %, emulsifiers concentration - 2.92 weight , antioxidants concentration - 0.02 weight %.

Example VIII

The following components are added into 16.83 kg of water: 1.49 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.42 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 1.26 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 3.10 kg withl48.15 g of sorbitan monooleate and63.49 g of polyoxyethylenesorbitan monooleate, and also with 168.51 g of rosemary extract. Next, 3.10 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.15. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion. Simultaneously, microbial bio mass of probiotic microorganisms is obtained - the mixture of probiotic bacteria Lactobacillus acidophilus and Pediococcus acidilactici - from pure microbial cultures at late logarithmic phase of growth by centrifugation at 4000 rpm. The mixture of live probiotic microorganisms: Lactobacillus acidophilus and Pediococcus acidilactici is introduced to the emulsion at the temperature of 30°C at the amount of, respectively, 5.55 10 ¹⁰ CFU (Colony Forming Units) and 4.28 x 10 ¹⁰ CFU per millilitre of the emulsion. The components are gently stirred obtaining homogenous emulsion. Spray- drying process is performed using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 79°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic microorganisms - the mixture of probiotic bacteria Lactobacillus acidophilus and Pediococcus acidilactici - their total number in final product being 6.67 x 10 ⁸ CFU per g of powder. This product is added to heated white sausage (Weisswurst) at the amount of 6g of powder per lOOg of meat. The parameters of obtained powder are as follows: total concentration of capsule components - 45.31 weight %, polymer concentration - 18.12 weight %, maltodextrin and/or glucose syrup concentration - 27.18 weight %, concentration of the source of n-3 fatty acids - 46.13 weight %, emulsifiers concentration - 3.15 weight %, antioxidants concentration - 2.5 weight %.

Example IX

The following components are added into 16.01 kg of water: 0.94 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.26 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 2.78 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 rnin) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 1.89 kg withl40.41 g of sorbitan monooieate and 60.18 g of polyoxyethylenesorbitan monooleate, and also with 77.59g of rosemary extract. Next, 2.14 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenizaiion, to receive primary emulsion. Simultaneously, microbial biomass of probiotic microorganisms is obtained - the mixture of probiotic bacteria Lactobacillus acidophilus and Pediococcus acidilactici - from pure microbial cultures at late logarithmic phase of growth by centrifugation at 4000 rpm. The mixture of live probiotic microorganisms: Lactobacillus acidophilus and Pediococcus acidilactici is introduced to the emulsion at the temperature of 30°C at the amount of, respectively, 5.55 x 10 ¹⁰ CFU (Colony Forming Units) and 4.28 x ! G ^W CFU per millilitre of the emulsion. The components are gently stirred obtaining homogenous emulsion. Spray- drying process is performed using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 79°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic microorganisms - the mixture of pro bio tic bacteria Lactobacillus acidophilus and Pediococcus acidilactici - their total number in final product being 6.67 x 10 ^s CFIJ per g of powder. This product is added to heated white sausage (Weisswurst) at the amount of 6g of powder per lOOg of meat. The parameters of obtained powder are as follows: total concentration of capsule components - 62.07 weight %, polymer concentration - 43.45 weight %, maltodextrin and/or glucose syrup concentration - 18.62 weight %, concentration of the source of n-3 fatty acids - 30.52 weight %, emulsifiers concentration - 3.24 weight %, antioxidants concentration - 1.25 weight %.

ExampleX

The following components are added into 15.99 kg of water: 1.57 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.44 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, and 2.00 kg of modified starch (95.5%' dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 1.89 kg with 140.41 g of sorbitan monooleate and 60.18 g of polyoxyethylenesorbitan monooleate, and also with 0.94 g of rosemary extract. Next, 2.07 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple high pressure homogenization at 2k bars, thus obtaining primary emulsion. Simultaneously, the biomass of probiotic yeasts is obtained from the pure culture of Saccharomyc.es boulardii at late logarithmic phase of growth, by centrifugation at 4500 rpm. Live probiotic yeast in form of centrifuged biomass of probiotic Saccharomyces boulardii are added to the emulsion at the temperature of 25 °C at the amount of 8.87 x 10 ⁹ CPU (Colony Forming Units) per millilitre of the emulsion (emulsion weight 22.07 kg). The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray- drier with pressure/pneumatic nozzle, at drying air temperature of 180°C and outlet temperature of 50°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic yeast Saccharo yces houlardii - their total number in final product being3.67 x 10 8 CPU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 62.99 weight %, polymer concentration - 31.5 weight %, maltodextrin and/or glucose syrup concentration - 31.5 weight %, concentration of the source of n-3 fatty acids - 30.8 weight %, emulsifiers concentration - 3.27 weight %, antioxidants concentration - 0.02 weight %.

Example XI

The following components are added into 16.01 kg of water: 0.94 kg of maltodextrin with DE of 19.9 and 95.0%dry mass residue, 0.26 kg of maltodextrin with DE of 11.9 and 95.0%dry mass residue, and 2.78 kg of modified starch (95.5% dry mass residue). The components are mixed intensively to form the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the oil from cod liver and evening primrose oil at the amount of 1kg and 0.98 kg, respectively, with 78.01 g of sorbitan monooleate and 33.43 g of polyoxyethylenesorbitan monooleate. To this mixture the following antioxidants are also added: oil of oregano and rosemary extract at weight ratio of 0.3, at the amount of 4.59 g and 15.37 g, respectively. Next, 2.09 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre- emulsion is subjected to triple mechanical homogenisation in 12 stage homogemzer, with shearing stress of 2kPa at the highest stage of homogenization, thus receiving the emulsion which is then subjected to high pressure homogenization (2kbars) in order to obtain primary emulsion. Simultaneously, the biomass of probiotic yeasts is obtained from the pure culture of Saccharomyces houlardii at late logarithmic phase of growth, by centrifugation at 4500 rpm. Live probiotic yeast in form of centrifuged biomass of probiotic Saccharomyces houlardii axe added to the emulsion at the temperature of 25 °C at the amount of 8.87 x 10 CPU (Colony Forming Units) per millilitre of the emulsion (emulsion weight 22.07 kg). The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with pressure/pneumatic nozzle, at drying air temperature of 180°C and outlet temperature of 50°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic yeast Saccharomyces boulardii - their total number in final product being 3.67 x 10 ^s CFU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 62.68 weight %, polymer concentration - 43.87 weight %, maltodextrin and/or glucose syrup concentration - 18.80 weight %, concentration of the source of n-3 fatty acids - 32.27 weight , emulsifiers concentration - 1.82 weight %, antioxidants concentration - 0.33 weight %.

Example XII

Emulsion is obtained as in example XI, except that the mixture of antioxidants is composed of: rosemary extract, oil of cloves and oil of oregano at weight ratio of 1/1/2, with the amount of each antioxidant being 4.99 g, 4.99 g and 9.98 g, respectively.

The parameters of obtained powder are as follows: total concentration of capsule components - 62.68 weight %, polymer concentration - 43.87 weight %, maltodextrin and/or glucose syrup concentration - 18.80 weight %, concentration of the source of n-3 fatty acids - 32.27 weight %, emulsifiers concentration - 1.82 weight %, antioxidants concentration - 0.33 weight %.

Example XIII

Emulsion is obtained as in example XII, except that instead of oil from cod liver - evening primrose oil is used. Additionally, the mixture of probiotic bacteria Lactobacillus plantarum 299v with probiotic bacteria Bacillus clausii are added to the emulsion at the temperature of 20°C at the amount of 1.34 x 10 ¹⁴ CFU and 1.13 x 10 ⁷ CFU per rnilliiitre of the emulsion, respectively. Spray-drying process is performed using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 82°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic microorganisms: the mixture of Lactobacillus plantarum299v and Bacillus clausii- their total number in final product being 7.67 x 10 ¹² CFU and 2.43 x 10° CFU per g of powder, respectively.

The parameters of obtained powder are as follows: total concentration of capsule components - 62.68 weight %, polymer concentration - 43.87 weight %, maltodextrin and/or glucose syrup concentration - 18.80 weight %, concentration of the source of n-3 fatty acids - 32.27 weight %, emulsifiers concentration - 1.82 weight %, antioxidants concentration - 0.33 weight %.

Example XIV

Emulsion is obtained as in example XII, except that instead of oil from cod liver - blackcurrant seed oil is used. Additionally, the probiotic bacteria Lactobacillus rhamnosus, obtained by filtration of the culture through the filter with pore size of 0.22 micrometers, are added to the emulsion at the temperature of 20°C at the amount of 7.26 x !0 ⁶ CFU per miililitre of the emulsion. Spray-drying process is performed using spray- drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 75°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic bacteria Lactobacillus rhamnosus- their total number in final product being 9.83 x 10 ³ CFU per g of powder.

The parameters of obtained powder are as follows: total concentration of capsule components - 62.68 weight %, polymer concentration - 43.87 weight %, maltodextrin and/or glucose syrup concentration - 18.80 weight %, concentration of the source of n-3 fatty acids - 32.27 weight %, emulsifiers concentration - 1.82 weight %, antioxidants concentration - 0.33 weight %.

Example XV

The following components are added into 15.51 kg of water: 0.36 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 1.44 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue, 0.69 kg of glucose syrup (80.9% dry mass residue), and 2.00 kg of modified starch (95.5% dry mass residue). The components are mixed intensively to form the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing 1.98 kg of oil from cod liver with 2.23 g of sorbitan monooleate and 111.44 g of soy lecithin. Also 9.92 g of rosemary extracts the antioxidants added to the mixture. Next, 2.08 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to single mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, thus receiving the emulsion which is then subjected to high pressure homogenization (2kbars) in order to obtain primary emulsion. Simultaneously, the biomass of probiotic yeasts is obtained from the pure culture of Saccharomyces boulardii at late logarithmic phase of growth, by centrifugation at 4500 rpm. Live probiotic yeast in form of centrifuged biomass of probiotic Saccharomyces boulardii are added to the emulsion at the temperature of 25°C at the amount of 8.87 x 10 ⁹ CFU (Colony Forming Units) per millilitre of the emulsion (emulsion weight 22.07 kg). The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with pressure/pneumatic nozzle, at drying air temperature of 160°C and outlet temperature of 85 °C, thus obtaining the powder in form of microcapsules containing immobilized probiotic yeast Saccharomyces boulardii - their total number in final product being 3.67 x 10 ^s CFU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 65.35 weight %, polymer concentration - 27.05 weight %, maltodextrin and/or glucose syrup concentration - 43.91 weight %, concentration of the source of n-3 fatty acids - 29.87 weight %, emulsifiers concentration - 0.03 weight %, antioxidants concentration - 0.15 weight %.

Example XVI

Emulsion is obtained as in example XXII, with the exception that the following components are added into 15.51 kg of water: 0.81 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, and 0.99 kg of maltodextrin with DE of 11.9 and 95.0% dry mass residue.

The parameters of obtained powder are as follows: total concentration of capsule components - 65.35 weight %, polymer concentration - 27.05 weight %, maltodextrin and/or glucose syrup concentration - 43.91 weight %, concentration of the source of n-3 fatty acids - 29.87 weight %, emulsifiers concentration - 0.03 weight %, antioxidants concentration - 0.15 weight %.

Example XVII

The following components are added into 16.01 kg of water: 0.94 kg of maltodextrin with DE of 19.9 and 95.0% dry mass residue, 0.26 kg of maltodextrin with DE of 1 1.9 and 95.0% dry mass residue, 2.78 kg of modified starch (95.5% dry mass residue), and mixture of hydrophilic colour additives in form of carrot juice concentrate and ammonia caramel at total amount of 0.22 kg. The components are mixed intensively to form the continuous phase. Simultaneously the dispersed phase is formed by short (10 min) mixing of the source of n-3 fatty acids from cod liver oil at the amount of 1.98 kg with 78.01 g of sorbitan monooieate and 33.43 g of polyoxyethylenesorbitan monooieate, and also with 0.94 g of rosemary extract. Next, 2.07 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in mixer with turbomixer and compartments. The pre-emulsion is obtained using nitrogen. Thus obtained pre- emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, thus receiving the emulsion which is then subjected to high pressure homogenization (2k bars) in order to obtain primary emulsion. Simultaneously, the biomass of probiotic yeasts is obtained from the pure culture of Saccharomyces boulardii at late logarithmic phase of growth, by centrifugation at 4500 rpm. Live probiotic yeast in form of centrifuged biomass of probiotic Saccharomyces boulardii are added to the emulsion at the temperature of 25°C at the amount of 8.87 x 10 CFU (Colony Forming Units) per rmllilitre of the emulsion (emulsion weight 22.07 kg). The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with pressure/pneumatic nozzle, at drying air temperature of 180°C and outlet temperature of 50°C, thus obtaining the powder in form of microcapsules containing immobilized probiotic yeast Saccharomyces boulardii - their total number in final product being 3.67 x 10 CFU per g of powder. The product is added to dumplings at the amount of 3.75g per lOOg of dumplings dough.

The parameters of obtained powder are as follows: total concentration of capsule components - 60.61 weigh %, polymer concentration - 42.43 weight %, maltodextrin and/or glucose syrup concentration - 18.18 weight %, concentration of the source of n-3 fatty acids - 31.2 weight %, emulsifiers concentration - 1.76 weight %, antioxidants concentration - 0.02 weight %, concentration of hydrophilic colour additives - 3.5 weight %. Example XVIII

The following components are added into 17.9 kg of water: 0.63 kg of glucose syrup and 1.5 kg of modified starch (95.5% dry mass residue). The components are turbulently mixed forming the continuous phase. Simultaneously the dispersed phase is formed by short mixing of the source of n-3 fatty acids from cod liver oil at the amount of 1.97 kg with 0.11 kg of polyoxyethylenesorbitan monooleate and with rosemary extract, oil of cloves and oregano extract at weight ratio of 1: 1: l and total weight of 0.99g. Next, 2.07 kg of dispersed phase is added to continuous phase and mixed intensively, thus obtaining the pre-emulsion with volumetric ratio of dispersed phase of 0.1. Both continuous and dispersed phases are formed in cylindrical jet-stream mixer with propeller agitator and wheel. The pre-emulsion is obtained using nitrogen. Thus obtained pre-emulsion is subjected to triple mechanical homogenisation in 12 stage homogenizer, with shearing stress of 2kPa at the highest stage of homogenization, to receive primary emulsion, and then subjected to one-step homogenization in high pressure homogenizer at the pressure of 2kbar. Simultaneously, microbial bio mass of probiotic bacteria is obtained - from the pure cultures of Lactobacillus rhamnosus and Lactobacillus plantarum at late logarithmic phase of growth by centrifugation at 4000 rpm. Live probiotic bacteria in form of mixture of Lactobacillus rhamnosus GG and Lactobacillus plantarum 29 v are added to the emulsion at the amount of 2.31 x 10 ¹⁰ CFU and 2.33 x 10 ^l0 CFU (Colony Forming Units), respectively, per millilitre of the emulsion. The components are stirred gently to obtain homogenous emulsion. Next, the emulsion is spray-dried using spray-drier with rotary atomizer, at drying air temperature of 180°C and outlet temperature of 72 °C, thus obtaining the powder in form of microcapsules containing immobilized probiotic bacteria Lactobacillus rhamnosus GG and Lactobacillus plantarum 299v - their total number in final product reaching 8.63 x 10 CFU per g of powder. The product is added to cottage cheese with onion and garlic at the amount of Ig per lOOg of food product.

The parameters of obtained powder are as follows: total concentration of capsule components - 46.83 weight %, concentration of the source of n-3 fatty acids - 45.80 weight %, emulsifiers concentration - 2.58 weight %, lecithin concentration - Ow weieht %, antioxidants concentration - 0.02 weieht %, concentration of colour additives - 0 weight %, emulsion weight - 22.07 kg.