Title:
PROCESS FOR EXTRACTION AND PURIFICATION OF PACLITAXEL FROM NATURAL SOURCES
Document Type and Number:
WIPO Patent Application WO2000078741
Kind Code:
A3
Abstract:
A process for the extraction and purification of Paclitaxel from a natural source of taxanes. This process comprises the following steps: a) extracting with an organic solvent, a raw material comprising Paclitaxel from said natural source of taxanes; b) treating said raw material with a base or an acid to obtain a biomass by precipitation, isolating said biomass and drying it; c) percolorizing the so-isolated biomass by removing resin and natural pigments contained therein, dissolving said biomass in acetone and then adding thereto at least one non-polar solvent until a Paclitaxel-enriched oily phase is obtained; d) treating with a base or an acid the biomass contained in the Paclitysel-enriched oily phase recovered in the preceding step to obtain another biomass by precipitation, isolating said other biomass and drying it; e) chromatographically purifying at least once a solution of the isolated other biomass obtained in the preceding step in a volatile solvent, and crystallizing at least once the purified solution obtained by chromatography. This process comprises a limited number of steps and allows, after filtration and drying, to obtain a mixture of Paclitaxel crystals consisting of: about 53% of crystals having a purity higher than 99%, about 22% of crystals having a purity higher than 98%, and about 23% of crystals having a purity hither than 92%.
Inventors:
BUI-KHAC TRUNG
DUPUIS NICOLAS
DUPUIS NICOLAS
Application Number:
PCT/CA2000/000619
Publication Date:
May 17, 2001
Filing Date:
May 25, 2000
Export Citation:
Assignee:
CHAICHEM PHARMACEUTICALS INTER (CA)
International Classes:
C07D305/14; (IPC1-7): C07D305/14
Domestic Patent References:
| WO1994013827A1 | 1994-06-23 |
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