Title:
A PROCESS OF MAKING TRANSGENIC MAMMALS THAT PRODUCE EXOGENOUS PROTEINS IN MILK AND TRANSGENIC MAMMALS PRODUCED THEREBY
Document Type and Number:
WIPO Patent Application WO2005033281
Kind Code:
A3
Abstract:
The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said mild from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.
Inventors:
MELO CARLOS ALBERTO (AR)
BARANAO LINO (AR)
BARANAO LINO (AR)
Application Number:
PCT/US2004/032019
Publication Date:
May 19, 2005
Filing Date:
September 29, 2004
Export Citation:
Assignee:
STERRENBELD BIOTECHNOLOGIE NORTH AMERICA INC (US)
MELO CARLOS ALBERTO (AR)
BARANAO LINO (AR)
MELO CARLOS ALBERTO (AR)
BARANAO LINO (AR)
International Classes:
A01K67/027; C07K1/00; C07K14/00; C07K14/61; C07K16/00; C07K17/00; C12N15/00; C12N15/85; C12N15/877; C12P21/00; C12N; (IPC1-7): C12N15/00; A01K67/027; C12P21/00
Foreign References:
| US6451527B1 | 2002-09-17 | |||
| US5741957A | 1998-04-21 | |||
| US6395958B1 | 2002-05-28 |
Other References:
CERDAN M.G. ET AL.: "Accurate spatial and temporal transgene expression driven by a 3.8 kilobase promoter of the bovine beta-casein gene in the lactatin mouse mammary gland", MOLECULAR REPRODUCTION AND DEVELOPMENT, vol. 49, 1998, pages 236 - 245, XP008045870
See also references of EP 1685245A4
See also references of EP 1685245A4
Download PDF: