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Title:
PROCESS FOR THE PREPARATION OF PHOSPHOROTHIONATE OLIGONUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO2002046205
Kind Code:
A3
Abstract:
A process for the synthesis of phosphorothioate oligonucleotides is provided which comprises assembling an aligonucleotide bound to to a solid support in the presence of acetonitrile; prior to cleaving the oligonucleotide from the solid support removing the acetonitrile; and cleaving the oligonucleotide from the solid support. The process is particularly suited to the large scale synthesis of nucleotides. The acetonitrile may be removed from the solid support by one or both to drying and by washing with solvents. Preferred washing solvents comprise trialkylamines.

Inventors:
DOUGLAS MARK EDWARD (GB)
MELLOR BEN JAMES (GB)
WELLINGS DONALD ALFRED (GB)
Application Number:
PCT/GB2001/005338
Publication Date:
August 15, 2002
Filing Date:
December 03, 2001
Export Citation:
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Assignee:
AVECIA LTD (GB)
DOUGLAS MARK EDWARD (GB)
MELLOR BEN JAMES (GB)
WELLINGS DONALD ALFRED (GB)
International Classes:
C07H21/00; (IPC1-7): C07H21/00
Domestic Patent References:
WO1997040458A21997-10-30
WO1992009615A11992-06-11
WO2000020431A12000-04-13
WO2001096358A12001-12-20
WO1994001446A21994-01-20
Foreign References:
US6111086A2000-08-29
EP0323152A21989-07-05
EP0288310A21988-10-26
US4753985A1988-06-28
Other References:
CHEMICAL ABSTRACTS, vol. 91, no. 25, 1979, Columbus, Ohio, US; abstract no. 211802, ARSHADY, REZA; ATHERTON, ERIC; GAIT, MICHAEL J.; LEE, KAREN; SHEPPARD, R.C.: "Easily prepared polar support for solid phase peptide and oligonucleotide synthesis. Preparation of substance P and a nonadeoxyribonucleotide" XP002197392
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