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Title:
PROCESS FOR PRODUCING GLUCOSE FROM STARCH EMPLOYING THE ASPERGILLUS CLAVATUS ALPHA-AMYLASE AND A PULLULANASE
Document Type and Number:
WIPO Patent Application WO/2014/028434
Kind Code:
A3
Abstract:
A fungal alpha amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30 - 75C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Inventors:
VAN BRUSSEL-ZWIJNEN MARCO (US)
HUITINK JACQUELYN A (US)
SCHEFFERS MARTIJN SILVAN (US)
TEUNISSEN PAULA JOHANNA MARIA (US)
VROEMEN CASPER (US)
Application Number:
PCT/US2013/054642
Publication Date:
May 15, 2014
Filing Date:
August 13, 2013
Export Citation:
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Assignee:
DANISCO US INC (US)
International Classes:
C12P19/02; A21D8/04; A23L7/104; A23L7/25; C11D3/386; C12C11/00; C12N9/30; C12N9/44; C12P7/06; C12P19/14; D06M16/00
Domestic Patent References:
WO2013169645A12013-11-14
WO2014028436A12014-02-20
Other References:
YAGONIA, C.F. & TABOADA, E.: "Enzymatic Hydrolysis of Cassava (Manihot esculenta) Starch", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 108, EP-08, November 2009 (2009-11-01), pages S99, XP026709512
DATABASE UniProt [online] 13 June 2012 (2012-06-13), FEDOROVA, N.D. ET AL.: "Alpha amylase, putative", XP002721376, Database accession no. A1CIW4
MONTEIRO DE SOUZA, P. & DE OLIVEIRA E MAGALHÃES, P.: "APPLICATION OF MICROBIAL alpha-AMYLASE IN INDUSTRY - A REVIEW", BRAZILIAN JOURNAL OF MICROBIOLOGY, vol. 41, no. 4, December 2010 (2010-12-01), pages 850 - 861, XP002689559
ADISA, A.: "Mycoflora of post-harvest maize and wheat grains and the implication of their contamination by molds", DIE NAHRUNG, vol. 38, no. 3, 1994, pages 318 - 326, XP002708070
Attorney, Agent or Firm:
BACSI, Steven, G. (925 Page Mill RoadPalo Alto, California, US)
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