Title:
PROCESS FOR PRODUCING GOUGEROTIN EMPLOYING STREPTOMYCES MICROFLAVUS STRAINS
Document Type and Number:
WIPO Patent Application WO/2014/059275
Kind Code:
A3
Abstract:
The present invention relates to novel strains of Streptomyces microflavus and methods of their use for controlling diseases or pests of a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin. The invention also relates to a method of producing a fermentation broth of a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin, the method comprising cultivating the Streptomyces strain in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at a concentration effective to achieve a gougerotin concentration of at least 1 g/L. The present disclosure also relates to the molecular cloning of a gougerotin biosynthetic gene cluster from Streptomyces microflavus, and identification of individual genes in the gene cluster as well as the proteins encoded thereby. A gougerotin gene cluster comprising 13 open reading frames (ORFs) is located within a genetic locus of Streptomyces microflavus.
Inventors:
CAMPBELL BRIAN (US)
CURTIS DAMIAN (US)
GUAN SHAOHUA (US)
GUILHABERT-GOYA MAGALIE (US)
JOO DANIEL M (US)
LU TARA (US)
MARGOLIS JONATHAN S (US)
ROYALTY REED NATHAN (US)
SALAZAR GERARDO BUENO (US)
SESIN DAVID (US)
SMITH FRISBY DAVIS (US)
TAYLOR COLLEEN (US)
ZHU HONG (US)
CURTIS DAMIAN (US)
GUAN SHAOHUA (US)
GUILHABERT-GOYA MAGALIE (US)
JOO DANIEL M (US)
LU TARA (US)
MARGOLIS JONATHAN S (US)
ROYALTY REED NATHAN (US)
SALAZAR GERARDO BUENO (US)
SESIN DAVID (US)
SMITH FRISBY DAVIS (US)
TAYLOR COLLEEN (US)
ZHU HONG (US)
Application Number:
PCT/US2013/064537
Publication Date:
October 30, 2014
Filing Date:
October 11, 2013
Export Citation:
Assignee:
BAYER CROPSCIENCE LP A DELAWARE LTD PARTNERSHIP (US)
International Classes:
C12P19/38; A01N43/54; A01N63/28; C07H19/06
Foreign References:
| CN103183729A | 2013-07-03 | |||
| US20140107061A1 | 2014-04-17 |
Other References:
ARAI, M. ET AL.: "ASPICULAMYCIN, A NEW CYTOSINE NUCLEOSIDE ANTIBIOTIC I. PRODUCING ORGANISM, FERMENTATION AND ISOLATION", JOURNAL OF ANTIBIOTICS, vol. 27, no. 5, May 1974 (1974-05-01), pages 329 - 333, XP008164495
DATABASE EPODOC [online] EUROPEAN PATENT OFFICE; 26 September 1978 (1978-09-26), "PREPARATION OF MITICIDE GOUGEROTIN", XP002712992, Database accession no. JP-2524777-A
DATABASE WPI Week 197128, Derwent World Patents Index; AN 1971-47413S, XP002723612, "Mitecidal gougerotin compsn"
DATABASE ENA [online] 9 October 2012 (2012-10-09), LIU, L. ET AL.: "Streptomyces graminearus strain AS4.506 gougerotin biosynthetic gene cluster, complete sequence", XP002724976, retrieved from EBI Database accession no. JQ307220 (Sequence Version Archive)
DATABASE NUCLEOTIDE [online] 10 October 2011 (2011-10-10), LUCAS, S. ET AL.: "Streptomyces flavogriseus ATCC 33331, complete genome", XP002724980, retrieved from EBI Database accession no. CP002475
NIU, G. ET AL.: "Cloning, Heterologous Expression, and Characterization of the Gene Cluster Required for Gougerotin Biosynthesis", CHEMISTRY & BIOLOGY, vol. 20, no. 1, 24 January 2013 (2013-01-24), pages 34 - 44, XP002712993
DATABASE EPODOC [online] EUROPEAN PATENT OFFICE; 26 September 1978 (1978-09-26), "PREPARATION OF MITICIDE GOUGEROTIN", XP002712992, Database accession no. JP-2524777-A
DATABASE WPI Week 197128, Derwent World Patents Index; AN 1971-47413S, XP002723612, "Mitecidal gougerotin compsn"
DATABASE ENA [online] 9 October 2012 (2012-10-09), LIU, L. ET AL.: "Streptomyces graminearus strain AS4.506 gougerotin biosynthetic gene cluster, complete sequence", XP002724976, retrieved from EBI Database accession no. JQ307220 (Sequence Version Archive)
DATABASE NUCLEOTIDE [online] 10 October 2011 (2011-10-10), LUCAS, S. ET AL.: "Streptomyces flavogriseus ATCC 33331, complete genome", XP002724980, retrieved from EBI Database accession no. CP002475
NIU, G. ET AL.: "Cloning, Heterologous Expression, and Characterization of the Gene Cluster Required for Gougerotin Biosynthesis", CHEMISTRY & BIOLOGY, vol. 20, no. 1, 24 January 2013 (2013-01-24), pages 34 - 44, XP002712993
Attorney, Agent or Firm:
SAMONEK, Michelle, L. (890 Embarcadero DriveWest Sacramento, CA, US)
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