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Title:
PROCESS FOR PRODUCING MICROBIAL TRANSGLUTAMINASE
Document Type and Number:
WIPO Patent Application WO/2004/078973
Kind Code:
A1
Abstract:
It is intended to provide an actinomyces-origin neutral metalloprotease, which selectively cleaves the pro-structure moiety of a microorganism-origin protransglutaminase, and a gene encoding the same. An activated microorganism-origin transglutaminase from which the pro-structural moiety has been excised can be produced by culturing a microorganism having the gene encoding the above-described actinomyces-origin neutral metalloprotease transferred thereinto, allowing the microorganism to produce the actinomyces-origin neutral metalloprotease and then treating the microorganism-origin protransglutaminase therewith.

Inventors:
UMEZAWA YUKIKO (JP)
YOKOYAMA KEIICHI (JP)
KIKUCHI YOSHIMI (JP)
DATE MASAYO (JP)
ONISHI NORIMASA (JP)
Application Number:
PCT/JP2004/002923
Publication Date:
September 16, 2004
Filing Date:
March 05, 2004
Export Citation:
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Assignee:
AJINOMOTO KK (JP)
UMEZAWA YUKIKO (JP)
YOKOYAMA KEIICHI (JP)
KIKUCHI YOSHIMI (JP)
DATE MASAYO (JP)
ONISHI NORIMASA (JP)
International Classes:
C12N9/10; C12N9/64; (IPC1-7): C12N15/09; C12N9/10; C12N9/52
Domestic Patent References:
WO2001023591A12001-04-05
Foreign References:
JPH0970291A1997-03-18
Other References:
KOJIMA S. ET AL: "Primary Structure of Streptomyces griseus Metalloendopeptidase II", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 62, no. 7, 1998, pages 1392 - 1398, XP002979775
KIKUCHI Y. ET AL: "Secretion of Active-Form Streptoverticillium mobaraense Transglutaminase by Corynebacterium glutamicum: Processing of the Pro-Transglutaminase by a Cosecreted Subtilisin-Like Protease from Streptomyces albogriseolus", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 69, no. 1, January 2003 (2003-01-01), pages 358 - 366, XP002979776
See also references of EP 1602722A4
NATURE, vol. 227, 1970, pages 380 - 685
Attorney, Agent or Firm:
Kumakura, Yoshio (3-1 Marunouchi 3-chome, Chiyoda-k, Tokyo 55, JP)
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