Title:
PROCESS FOR PRODUCING MONATIN
Document Type and Number:
WIPO Patent Application WO/2012/147674
Kind Code:
A1
Abstract:
The present invention provides: a process for producing 2R,4R-monatin with good yield not using an expensive starting material D-Trp but using an inexpensive starting material L-Trp; and others. Specifically, the present invention provides: a process for producing 2R,4R-monatin or a salt thereof, which comprises the steps (1) to (3) mentioned below; and others. (1) A step of bringing L-tryptophan into contact with a deaminase to produce indole-3-pyruvic acid; (2) a step of bringing indole-3-pyruvic acid and pyruvic acid into contact with aldolase to produce 4R-IHOG; and (3) a step of bringing 4R-IHOG into contact with a D-aminotransferase in the presence of a D-amino acid to produce 2R,4R-monatin. In the step (3), it is preferred to use a D-aminotransferase that does not have an ability of producing D-tryptophan from indole-3-pyruvic acid or is reduced in the ability.
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Inventors:
SUGIYAMA MASAKAZU (JP)
TAKAKURA YASUAKI (JP)
MORIYA MIKA (JP)
HAGIWARA YUSUKE (JP)
TABUCHI ERI (JP)
TAKAKURA YASUAKI (JP)
MORIYA MIKA (JP)
HAGIWARA YUSUKE (JP)
TABUCHI ERI (JP)
Application Number:
PCT/JP2012/060824
Publication Date:
November 01, 2012
Filing Date:
April 23, 2012
Export Citation:
Assignee:
AJINOMOTO KK (JP)
SUGIYAMA MASAKAZU (JP)
TAKAKURA YASUAKI (JP)
MORIYA MIKA (JP)
HAGIWARA YUSUKE (JP)
TABUCHI ERI (JP)
SUGIYAMA MASAKAZU (JP)
TAKAKURA YASUAKI (JP)
MORIYA MIKA (JP)
HAGIWARA YUSUKE (JP)
TABUCHI ERI (JP)
International Classes:
C12N15/09; C12N9/10; C12P17/10
Domestic Patent References:
| WO2004053125A1 | 2004-06-24 |
Foreign References:
| JP2010507367A | 2010-03-11 | |||
| JP2009535028A | 2009-10-01 | |||
| JP2007508840A | 2007-04-12 | |||
| JP2005523696A | 2005-08-11 | |||
| JP2008538932A | 2008-11-13 | |||
| JPS6091993A | 1985-05-23 |
Other References:
SUGIO, S. ET AL.: "Crystal structure of a D- amino acid aminotransferase: how the protein controls stereoselectivity", BIOCHEMISTRY, vol. 34, 1995, pages 9661 - 9669
RO, H.-S. ET AL.: "Site-directed mutagenesis of the amino acid residues in p-strand III [Val30- Val36] of D-amino acid aminotransferase of Bacillus sp. YM-1", FEBS LETTERS, vol. 398, 1996, pages 141 - 145
RO, H.-S. ET AL.: "Site-directed mutagenesis of the amino acid residues in p-strand III [Val30- Val36] of D-amino acid aminotransferase of Bacillus sp. YM-1", FEBS LETTERS, vol. 398, 1996, pages 141 - 145
Attorney, Agent or Firm:
SAKAI, Hiroaki et al. (JP)
Hiroaki Sakai (JP)
Hiroaki Sakai (JP)
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