PROCESS FOR PRODUCING PROTEIN IN CELL-FREE PROTEIN SYNTHESIS SYSTEM USING THIOREDOXIN FUSED PROTEIN EXPRESSION VECTOR

Title:

PROCESS FOR PRODUCING PROTEIN IN CELL-FREE PROTEIN SYNTHESIS SYSTEM USING THIOREDOXIN FUSED PROTEIN EXPRESSION VECTOR

Document Type and Number:

WIPO Patent Application WO/2003/097829

Kind Code:

A1

Abstract:

A fused protein of thioredoxin with a target protein is expressed in a cell-free protein synthesis system to thereby synthesize at least a part of the above-described fused protein as a soluble protein. It is preferable that the target protein is a highly hydrophobic protein such as a membrane protein. In particular, a membrane protein such a G protein-coupled receptor can be produced in a state being highly soluble and capable of forming a biologically active stereostructure. Also, a recombinant vector for producing such a membrane protein in a cell-free protein synthesis system is provided.

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Inventors:

SHIROUZU MIKAKO (JP)
ISHIHARA GOUSHI (JP)
SAEKI MIHORO (JP)
GOTO MIE (JP)
TAJIMA KAORI (JP)
KIGAWA TAKANORI (JP)
YOKOYAMA SHIGEYUKI (JP)

Application Number:

PCT/JP2003/006314

Publication Date:

November 27, 2003

Filing Date:

May 21, 2003

Export Citation:

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Assignee:

RIKEN (JP)
SHIROUZU MIKAKO (JP)
ISHIHARA GOUSHI (JP)
SAEKI MIHORO (JP)
GOTO MIE (JP)
TAJIMA KAORI (JP)
KIGAWA TAKANORI (JP)
YOKOYAMA SHIGEYUKI (JP)

International Classes:

C07K14/705; C12N9/02; C12N15/62; C12P21/00; C12P21/02; (IPC1-7): C12N15/09; C12P21/02; C07K19/00

Domestic Patent References:

WO1992013955A1	1992-08-20
WO2000068412A1	2000-11-16
WO2002090537A1	2002-11-14
WO1992013955A1	1992-08-20
WO2002095037A1	2002-11-28

Foreign References:

US20020142387A1

2002-10-03

Other References:

PENG S. ET AL.: "FEBS LETTERS", vol. 431, ELSEVIER SCIENCE PUBLISHERS, pages: 29 - 33
See also references of EP 1507003A4

Attorney, Agent or Firm:

Kato, Asamichi (Bohsei Bldg. 7th Floor, 20-12, Shin-Yokohama 3-chome, Kohoku-k, Yokohama-shi Kanagawa, JP)

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