Title:
PROCESS FOR THE PRODUCTION OF L-AMINO ACIDS USING CORYNEFORM BACTERIA
Document Type and Number:
WIPO Patent Application WO2003023044
Kind Code:
A3
Abstract:
The invention relates to a process for the production of L-amino acids, in which the following steps are carried out: a) fermentation of the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for S-adenosylmethionine synthetase and/or the gene coding for a transporter for branched-chain amino acids is/are attenuated, b) enrichment of the desired L-amino acid in the medium or in the bacterial cells, and c) isolation of the L-amino acid, and optionally bacteria are used in which in addition further genes of the biosynthesis pathway of the desired L-amino acid are enhanced, or bacteria are used in which the metabolic pathways that reduce the formation of the desired L-amino acid are at least partially switched off.
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Inventors:
BATHE BRIGITTE (DE)
PFEFFERLE WALTER (DE)
HUTHMACHER KLAUS (DE)
PFEFFERLE WALTER (DE)
HUTHMACHER KLAUS (DE)
Application Number:
PCT/EP2002/009043
Publication Date:
August 14, 2003
Filing Date:
August 13, 2002
Export Citation:
Assignee:
DEGUSSA (DE)
BATHE BRIGITTE (DE)
PFEFFERLE WALTER (DE)
HUTHMACHER KLAUS (DE)
BATHE BRIGITTE (DE)
PFEFFERLE WALTER (DE)
HUTHMACHER KLAUS (DE)
International Classes:
C12N1/21; C12P13/12; (IPC1-7): C12P13/04; C12N1/21; C12P13/12
Other References:
NAKAMORI S ET AL: "Mechanism of L-methionine overproduction by Escherichia coli: The replacement of Ser-54 by Asn in the MetJ protein causes the derepression of L-methionine biosynthetic enzymes.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 52, no. 2, August 1999 (1999-08-01), pages 179 - 185, XP002242975, ISSN: 0175-7598
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 08 6 October 2000 (2000-10-06)
TAUCH A ET AL: "ISOLEUCINE UPTAKE IN CORYNEBACTERIUM GLUTAMICUM ATCC 13032 IS DIRECTED BY THE BRNQ GENE PRODUCT", ARCHIVES OF MICROBIOLOGY, BERLIN, DE, vol. 169, no. 4, 1998, pages 303 - 312, XP000979580, ISSN: 0302-8933
SEKOWSKA AGNIESZKA ET AL: "Sulfur metabolism in Escherichia coli and related bacteria: Facts and fiction.", JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, vol. 2, no. 2, April 2000 (2000-04-01), April, 2000, pages 145 - 177, XP002242976, ISSN: 1464-1801
GOTO DEREK B ET AL: "A single-nucleotide mutation in a gene encoding S-adenosylmethionine synthetase is associated with methionine over-accumulation phenotype in Arabidopsis thaliana.", GENES & GENETIC SYSTEMS, vol. 77, no. 2, April 2002 (2002-04-01), pages 89 - 95, XP002242977, ISSN: 1341-7568
GROSSMANN K ET AL: "RAPID CLONING OF METK ENCODING METHIONINE ADENOSYLTRANSFERASE FROM CORYNEBACTERIUM GLUTAMICUM BY SCREENING A GENOMIC LIBRARY ON A HIGHDENSITY COLONY-ARRAY", FEMS MICROBIOLOGY LETTERS, AMSTERDAM, NL, vol. 193, no. 1, 1 December 2000 (2000-12-01), pages 99 - 103, XP000984551, ISSN: 0378-1097
ANGELO S ET AL: "The binding specificity of amino acid transport system y+L in human erythrocytes is altered by monovalent cations.", JOURNAL OF MEMBRANE BIOLOGY, vol. 153, no. 1, 1996, pages 37 - 44, XP002242978, ISSN: 0022-2631
NEIDHARDT F. (ED.), GREENE R.: "Escherichia coli and Salmonella, Cellular and molecular biology (Passage: Biosynthesis of Methionine)", 1996, ASM PRESS, WASHINGTON, US, ISBN: 1-55581-084-5, XP002242979
OHNISHI K ET AL: "Identification of a cis-acting regulatory sequence responsible for the repression of brnQ in Salmonella typhimurium", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1445, no. 2, 14 May 1999 (1999-05-14), pages 196 - 206, XP004275377, ISSN: 0167-4781
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 08 6 October 2000 (2000-10-06)
TAUCH A ET AL: "ISOLEUCINE UPTAKE IN CORYNEBACTERIUM GLUTAMICUM ATCC 13032 IS DIRECTED BY THE BRNQ GENE PRODUCT", ARCHIVES OF MICROBIOLOGY, BERLIN, DE, vol. 169, no. 4, 1998, pages 303 - 312, XP000979580, ISSN: 0302-8933
SEKOWSKA AGNIESZKA ET AL: "Sulfur metabolism in Escherichia coli and related bacteria: Facts and fiction.", JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, vol. 2, no. 2, April 2000 (2000-04-01), April, 2000, pages 145 - 177, XP002242976, ISSN: 1464-1801
GOTO DEREK B ET AL: "A single-nucleotide mutation in a gene encoding S-adenosylmethionine synthetase is associated with methionine over-accumulation phenotype in Arabidopsis thaliana.", GENES & GENETIC SYSTEMS, vol. 77, no. 2, April 2002 (2002-04-01), pages 89 - 95, XP002242977, ISSN: 1341-7568
GROSSMANN K ET AL: "RAPID CLONING OF METK ENCODING METHIONINE ADENOSYLTRANSFERASE FROM CORYNEBACTERIUM GLUTAMICUM BY SCREENING A GENOMIC LIBRARY ON A HIGHDENSITY COLONY-ARRAY", FEMS MICROBIOLOGY LETTERS, AMSTERDAM, NL, vol. 193, no. 1, 1 December 2000 (2000-12-01), pages 99 - 103, XP000984551, ISSN: 0378-1097
ANGELO S ET AL: "The binding specificity of amino acid transport system y+L in human erythrocytes is altered by monovalent cations.", JOURNAL OF MEMBRANE BIOLOGY, vol. 153, no. 1, 1996, pages 37 - 44, XP002242978, ISSN: 0022-2631
NEIDHARDT F. (ED.), GREENE R.: "Escherichia coli and Salmonella, Cellular and molecular biology (Passage: Biosynthesis of Methionine)", 1996, ASM PRESS, WASHINGTON, US, ISBN: 1-55581-084-5, XP002242979
OHNISHI K ET AL: "Identification of a cis-acting regulatory sequence responsible for the repression of brnQ in Salmonella typhimurium", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1445, no. 2, 14 May 1999 (1999-05-14), pages 196 - 206, XP004275377, ISSN: 0167-4781
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